LOCUS       ALR14549.1               490 aa    PRT              BCT 02-SEP-2016
DEFINITION  Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 RNA
            polymerase factor sigma-54 protein.
ACCESSION   CP009977-75
PROTEIN_ID  ALR14549.1
SOURCE      Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759
  ORGANISM  Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759
            Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
            Vibrionaceae; Vibrio.
REFERENCE   1  (bases 1 to 3248023)
  AUTHORS   Lee,H. and Church,G.M.
  TITLE     Vibrio natriegens genome v1.0
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3248023)
  AUTHORS   Lee,H. and Church,G.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-NOV-2014) Genetics, Harvard Medical School, 77 Avenue
            Louis Pasteur, NRB 233, Boston, MA 02115, USA
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: RS HGAP v. 3
            Assembly Name          :: Vnat1.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 96.48x
            Sequencing Technology  :: PacBio
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 11/24/2014 10:39:19
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
                                            Pipeline
            Annotation Method            :: Best-placed reference protein set;
                                            GeneMarkS+
            Annotation Software revision :: 2.9 (rev. 452286)
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA;
                                            repeat_region
            Genes                        :: 4,665
            CDS                          :: 4,459
            Pseudo Genes                 :: 42
            rRNAs                        :: 34 (5S, 16S, 23S)
            tRNAs                        :: 129
            ncRNA                        :: 1
            Frameshifted Genes           :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Qualifiers
     source          /organism="Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM
                     759"
                     /mol_type="genomic DNA"
                     /strain="ATCC 14048"
                     /isolation_source="salt marsh mud"
                     /culture_collection="ATCC:14048"
                     /type_material="type strain of Vibrio natriegens"
                     /db_xref="taxon:1219067"
                     /chromosome="1"
                     /country="USA"
                     /collection_date="27-Feb-2012"
     protein         /locus_tag="PN96_00490"
                     /EC_number="2.7.7.6"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_014233079.1"
                     /note="sigma factors are initiation factors that promote
                     the attachment of RNA polymerase to specific initiation
                     sites and are then released; sigma 54 factor is
                     responsible for the expression of enzymes involved in
                     nitrogen assimilation and metabolism; the rhizobia often
                     have 2 copies of this sigma factor; in Rhizobium etli
                     RpoN1 shown to be involved in the assimilation of several
                     nitrogen and carbon sources during free-living aerobic
                     growth and RpoN2 is involved in symbiotic nitrogen
                     fixation; in Bradyrhizobium both RpoN1 and N2 are
                     functional in free-living and symbiotic conditions, rpoN1
                     gene was regulated in response to oxygen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /transl_table=11
BEGIN
        1 MKPSLQLKLG QQLAMTPQLQ QAIRLLQLST LDLQQEIQEA LDSNPLLEVE DGQDEPLANG
       61 EDKSELDASE NSTSANEPTE IDVPDSSDVI EKSEISSELE IDTTWDDVYS ANTGSTGLAL
      121 DDDMPVYQGE TTESLYDYLM WQLDLTPFSE TDRTIAMAII DAVDEYGYLT LSPEEIHESF
      181 DNEDIELDEV EAVRKRIQQF DPLGVASRNL QECLLLQLAT FPEDTPWLEE ARMILTDHID
      241 HLGNRDYKLV IKETKLKEAD LREVLKLIQQ LDPRPGSRIT PDDTEYVIPD VSVFKDNGKW
      301 MVSINPDSIP KLKVNQQYAQ LGKGNSADSQ YIRSNLQEAK WLIKSLESRN ETLLKVARCI
      361 VEHQQDFFEY GEEAMKPMVL NDVALAVDMH ESTISRVTTQ KFMHTPRGIF ELKYFFSSHV
      421 STDNGGECSS TAIRALIKKL VAAENTAKPL SDSKIAALLA DQGIQVARRT IAKYRESLGI
      481 APSSQRKRLL
//