LOCUS ALR14549.1 490 aa PRT BCT 02-SEP-2016 DEFINITION Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 RNA polymerase factor sigma-54 protein. ACCESSION CP009977-75 PROTEIN_ID ALR14549.1 SOURCE Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 ORGANISM Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio. REFERENCE 1 (bases 1 to 3248023) AUTHORS Lee,H. and Church,G.M. TITLE Vibrio natriegens genome v1.0 JOURNAL Unpublished REFERENCE 2 (bases 1 to 3248023) AUTHORS Lee,H. and Church,G.M. TITLE Direct Submission JOURNAL Submitted (21-NOV-2014) Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, NRB 233, Boston, MA 02115, USA COMMENT Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/ ##Genome-Assembly-Data-START## Assembly Method :: RS HGAP v. 3 Assembly Name :: Vnat1.0 Genome Representation :: Full Expected Final Version :: Yes Genome Coverage :: 96.48x Sequencing Technology :: PacBio ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 11/24/2014 10:39:19 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS+ Annotation Software revision :: 2.9 (rev. 452286) Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes :: 4,665 CDS :: 4,459 Pseudo Genes :: 42 rRNAs :: 34 (5S, 16S, 23S) tRNAs :: 129 ncRNA :: 1 Frameshifted Genes :: 10 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759" /mol_type="genomic DNA" /strain="ATCC 14048" /isolation_source="salt marsh mud" /culture_collection="ATCC:14048" /type_material="type strain of Vibrio natriegens" /db_xref="taxon:1219067" /chromosome="1" /country="USA" /collection_date="27-Feb-2012" protein /locus_tag="PN96_00490" /EC_number="2.7.7.6" /inference="EXISTENCE: similar to AA sequence:RefSeq:WP_014233079.1" /note="sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released; sigma 54 factor is responsible for the expression of enzymes involved in nitrogen assimilation and metabolism; the rhizobia often have 2 copies of this sigma factor; in Rhizobium etli RpoN1 shown to be involved in the assimilation of several nitrogen and carbon sources during free-living aerobic growth and RpoN2 is involved in symbiotic nitrogen fixation; in Bradyrhizobium both RpoN1 and N2 are functional in free-living and symbiotic conditions, rpoN1 gene was regulated in response to oxygen; Derived by automated computational analysis using gene prediction method: Protein Homology." /transl_table=11 BEGIN 1 MKPSLQLKLG QQLAMTPQLQ QAIRLLQLST LDLQQEIQEA LDSNPLLEVE DGQDEPLANG 61 EDKSELDASE NSTSANEPTE IDVPDSSDVI EKSEISSELE IDTTWDDVYS ANTGSTGLAL 121 DDDMPVYQGE TTESLYDYLM WQLDLTPFSE TDRTIAMAII DAVDEYGYLT LSPEEIHESF 181 DNEDIELDEV EAVRKRIQQF DPLGVASRNL QECLLLQLAT FPEDTPWLEE ARMILTDHID 241 HLGNRDYKLV IKETKLKEAD LREVLKLIQQ LDPRPGSRIT PDDTEYVIPD VSVFKDNGKW 301 MVSINPDSIP KLKVNQQYAQ LGKGNSADSQ YIRSNLQEAK WLIKSLESRN ETLLKVARCI 361 VEHQQDFFEY GEEAMKPMVL NDVALAVDMH ESTISRVTTQ KFMHTPRGIF ELKYFFSSHV 421 STDNGGECSS TAIRALIKKL VAAENTAKPL SDSKIAALLA DQGIQVARRT IAKYRESLGI 481 APSSQRKRLL //