LOCUS AJF01565.1 337 aa PRT BCT 20-JUN-2017 DEFINITION Mycobacterium tuberculosis H37RvSiena Double hotdog hydratase protein. ACCESSION CP007027-221 PROTEIN_ID AJF01565.1 SOURCE Mycobacterium tuberculosis H37RvSiena ORGANISM Mycobacterium tuberculosis H37RvSiena Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 (bases 1 to 4410911) AUTHORS Santoro,F., Guerrini,V., Lazzeri,E., Iannelli,F. and Pozzi,G. TITLE Genomic polymorphisms in a Laboratory Isolate of Mycobacterium tuberculosis Reference Strain H37Rv (ATCC27294) JOURNAL New Microbiol. 40 (1), 62-69 (2017) PUBMED 27819398 REFERENCE 2 (bases 1 to 4410911) AUTHORS Guerrini,V., Santoro,F. and Pozzi,G. TITLE Direct Submission JOURNAL Submitted (30-DEC-2013) Medical Biotechnology, University of Siena, viale Bracci, Policlinico Le Scotte, Siena 53100, Italy REFERENCE 3 (bases 1 to 4410911) AUTHORS Guerrini,V., Santoro,F. and Pozzi,G. TITLE Direct Submission JOURNAL Submitted (20-FEB-2015) Medical Biotechnology, University of Siena, viale Bracci, Policlinico Le Scotte, Siena 53100, Italy REMARK Protein update by submitter COMMENT Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/ Annotation modified by submitter. ##Assembly-Data-START## Assembly Method :: Ray v. 2.3.1 Coverage :: 300 Sequencing Technology :: Illumina ##Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Date :: 12/30/2013 09:28:23 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline Annotation Method :: Best-placed reference protein set; GeneMarkS+ Annotation Software revision :: 2.3 (rev. 422554) Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes :: 4,146 CDS :: 4,050 Pseudo Genes :: 42 CRISPR Arrays :: 2 rRNAs :: 3 (5S, 16S, 23S) tRNAs :: 45 ncRNA :: 6 Frameshifted Genes :: 34 ##Genome-Annotation-Data-END## FEATURES Qualifiers source /organism="Mycobacterium tuberculosis H37RvSiena" /mol_type="genomic DNA" /strain="H37RvSiena" /db_xref="taxon:1437856" protein /locus_tag="Y980_0216" /experiment="EXISTENCE: identified in proteomics study" /note="Rv0216, (MTCY08D5.11) Double hotdog R-specific hydratase of unknown function, shows no activity for crotonyl-CoA, equivalent to Z95398|MLCL622_22 from Mycobacterium leprae (339 aa). Shows structural similarity to six others in Mycobacterium tuberculosis (see Castell et al (2005) below). A core mycobacterial gene; conserved in mycobacterial strains (See Marmiesse et al., 2004)." /transl_table=11 /db_xref="GOA:P96398" /db_xref="InterPro:IPR016790" /db_xref="PDB:2BI0" /db_xref="UniProtKB/TrEMBL:P96398" BEGIN 1 MASGYGGIRV GGPYFDDLSK GQVFDWAPGV TLSLGLAAAH QSIVGNRLRL ALDSDLCAAV 61 TGMPGPLAHP GLVCDVAIGQ STLATQRVKA NLFYRGLRFH RFPAVGDTLY TRTEVVGLRA 121 NSPKPGRAPT GLAGLRMTTI DRTDRLVLDF YRCAMLPASP DWKPGAVPGD DLSRIGADAP 181 APAADPTAHW DGAVFRKRVP GPHFDAGIAG AVLHSTADLV SGAPELARLT LNIAATHHDW 241 RVSGRRLVYG GHTIGLALAQ ATRLLPNLAT VLDWESCDHT APVHEGDTLY SELHIESAQA 301 HADGGVLGLR SLVYAVSDSA SEPDRQVLDW RFSALQF //