LOCUS AJF01447.1 2512 aa PRT BCT 20-JUN-2017
DEFINITION Mycobacterium tuberculosis H37RvSiena putative peptide
synthetase Nrp (peptide synthase) protein.
ACCESSION CP007027-103
PROTEIN_ID AJF01447.1
SOURCE Mycobacterium tuberculosis H37RvSiena
ORGANISM Mycobacterium tuberculosis H37RvSiena
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1 (bases 1 to 4410911)
AUTHORS Santoro,F., Guerrini,V., Lazzeri,E., Iannelli,F. and Pozzi,G.
TITLE Genomic polymorphisms in a Laboratory Isolate of Mycobacterium
tuberculosis Reference Strain H37Rv (ATCC27294)
JOURNAL New Microbiol. 40 (1), 62-69 (2017)
PUBMED 27819398
REFERENCE 2 (bases 1 to 4410911)
AUTHORS Guerrini,V., Santoro,F. and Pozzi,G.
TITLE Direct Submission
JOURNAL Submitted (30-DEC-2013) Medical Biotechnology, University of Siena,
viale Bracci, Policlinico Le Scotte, Siena 53100, Italy
REFERENCE 3 (bases 1 to 4410911)
AUTHORS Guerrini,V., Santoro,F. and Pozzi,G.
TITLE Direct Submission
JOURNAL Submitted (20-FEB-2015) Medical Biotechnology, University of Siena,
viale Bracci, Policlinico Le Scotte, Siena 53100, Italy
REMARK Protein update by submitter
COMMENT Annotation was added by the NCBI Prokaryotic Genome Annotation
Pipeline (released 2013). Information about the Pipeline can be
found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/
Annotation modified by submitter.
##Assembly-Data-START##
Assembly Method :: Ray v. 2.3.1
Coverage :: 300
Sequencing Technology :: Illumina
##Assembly-Data-END##
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Date :: 12/30/2013 09:28:23
Annotation Pipeline :: NCBI Prokaryotic Genome Annotation
Pipeline
Annotation Method :: Best-placed reference protein set;
GeneMarkS+
Annotation Software revision :: 2.3 (rev. 422554)
Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA;
repeat_region
Genes :: 4,146
CDS :: 4,050
Pseudo Genes :: 42
CRISPR Arrays :: 2
rRNAs :: 3 (5S, 16S, 23S)
tRNAs :: 45
ncRNA :: 6
Frameshifted Genes :: 34
##Genome-Annotation-Data-END##
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis H37RvSiena"
/mol_type="genomic DNA"
/strain="H37RvSiena"
/db_xref="taxon:1437856"
protein /gene="nrp"
/locus_tag="Y980_0101"
/experiment="EXISTENCE: identified in proteomics study"
/inference="protein motif:PROSITE:PS00012"
/inference="protein motif:PROSITE:PS00017"
/inference="protein motif:PROSITE:PS00455"
/note="Rv0101, (MTCY251.20) Probable nrp,peptide
synthetase, similar to others e.g.
AAD44234.1|AF143772_40|PstB peptide synthetase from
Mycobacterium avium (2552 aa); 7476034|S77657 cyclic
peptide synthetase from Mycobacterium leprae (1401 aa);
part of CAB55600.1|AJ238027 peptide synthetase from
Mycobacterium smegmatis (5990). Also similar to e.g.
AAD56240.1|AF184977_1|AF184977 DhbF protein from Bacillus
subtilis (2378 aa); SRF1_BACSU|P27206 surfactin synthetase
subunit 1 (3587 aa): etc. Contains PS00017 ATP/GTP-binding
site motif A (P-loop), 2 x PS00455 Putative AMP-binding
domain signature, and PS00012 Phosphopantetheine
attachment site. Belongs to the ATP-dependent AMP-binding
enzyme family. Thought to be not involved in mycobactin
biosynthesis (see citation below)."
/transl_table=11
/db_xref="GOA:Q10896"
/db_xref="HSSP:O30409"
/db_xref="InterPro:IPR000873"
/db_xref="InterPro:IPR001242"
/db_xref="InterPro:IPR006162"
/db_xref="InterPro:IPR009081"
/db_xref="InterPro:IPR010071"
/db_xref="InterPro:IPR010080"
/db_xref="InterPro:IPR013120"
/db_xref="InterPro:IPR016040"
/db_xref="InterPro:IPR020845"
/db_xref="PDB:4DQV"
/db_xref="UniProtKB/TrEMBL:Q10896"
BEGIN
1 MHRVRLSRSQ RNLYNGVRQD NNPALYLIGK SYRFRRLELA RFLAALHATV LDNPVQLCVL
61 ENSGADYPDL VPRLRFGDIV RVGSADEHLQ STWCSGILGK PLVRHTVHTD PNGYVTGLDV
121 HTHHILLDGG ATGTIEADLA RYLTTDPAGE TPSVGAGLAK LREAHRRETA KVEESRGRLS
181 AVVQRELADE AYHGGHGHSV SDAPGTAAKG VLHESATICG NAFDAILTLS EAQRVPLNVL
241 VAAAAVAVDA SLRQNTETLL VHTVDNRFGD SDLNVATCLV NSVAQTVRFP PFASVSDVVR
301 TLDRGYVKAV RRRWLREEHY RRMYLAINRT SHVEALTLNF IREPCAPGLR PFLSEVPIAT
361 DIGPVEGMTV ASVLDEEQRT LNLAIWNRAD LPACKTHPKV AERIAAALES MAAMWDRPIA
421 MIVNDWFGIG PDGTRCQGDW PARQPSTPAW FLDSARGVHQ FLGRRRFVYP WVAWLVQRGA
481 APGDVLVFTD DDTDKTIDLL IACHLAGCGY SVCDTADEIS VRTNAITEHG DGILVTVVDV
541 AATQLAVVGH DELRKVVDER VTQVTHDALL ATKTAYIMPT SGTTGQPKLV RISHGSLAVF
601 CDAISRAYGW GAHDTVLQCA PLTSDISVEE IFGGAACGAR LVRSAAMKTG DLAALVDDLV
661 ARETTIVDLP TAVWQLLCAD GDAIDAIGRS RLRQIVIGGE AIRCSAVDKW LESAASQGIS
721 LLSSYGPTEA TVVATFLPIV CDQTTMDGAL LRLGRPILPN TVFLAFGEVV IVGDLVADGY
781 LGIDGDGFGT VTAADGSRRR AFATGDRVTV DAEGFPVFSG RKDAVVKISG KRVDIAEVTR
841 RIAEDPAVSD VAVELHSGSL GVWFKSQRTR EGEQDAAAAT RIRLVLVSLG VSSFFVVGVP
901 NIPRKPNGKI DSDNLPRLPQ WSAAGLNTAE TGQRAAGLSQ IWSRQLGRAI GPDSSLLGEG
961 IGSLDLIRIL PETRRYLGWR LSLLDLIGAD TAANLADYAP TPDAPTGEDR FRPLVAAQRP
1021 AAIPLSFAQR RLWFLDQLQR PAPVYNMAVA LRLRGYLDTE ALGAAVADVV GRHESLRTVF
1081 PAVDGVPRQL VIEARRADLG CDIVDATAWP ADRLQRAIEE AARHSFDLAT EIPLRTWLFR
1141 IADDEHVLVA VAHHIAADGW SVAPLTADLS AAYASRCAGR APDWAPLPVQ YVDYTLWQRE
1201 ILGDLDDSDS PIAAQLAYWE NALAGMPERL RLPTARPYPP VADQRGASLV VDWPASVQQQ
1261 VRRIARQHNA TSFMVVAAGL AVLLSKLSGS PDVAVGFPIA GRSDPALDNL VGFFVNTLVL
1321 RVNLAGDPSF AELLGQVRAR SLAAYENQDV PFEVLVDRLK PTRALTHHPL IQVMLAWQDN
1381 PVGQLNLGDL QATPMPIDTR TARMDLVFSL AERFSEGSEP AGIGGAVEYR TDVFEAQAID
1441 VLIERLRKVL VAVAAAPERT VSSIDALDGT ERARLDEWGN RAVLTAPAPT PVSIPQMLAA
1501 QVARIPEAEA VCCGDASMTY RELDEASNRL AHRLAGCGAG PGECVALLFE RCAPAVVAMV
1561 AVLKTGAAYL PIDPANPPPR VAFMLGDAVP VAAVTTAGLR SRLAGHDLPI IDVVDALAAY
1621 PGTPPPMPAA VNLAYILYTS GTTGEPKGVG ITHRNVTRLF ASLPARLSAA QVWSQCHSYG
1681 FDASAWEIWG ALLGGGRLVI VPESVAASPN DFHGLLVAEH VSVLTQTPAA VAMLPTQGLE
1741 SVALVVAGEA CPAALVDRWA PGRVMLNAYG PTETTICAAI SAPLRPGSGM PPIGVPVSGA
1801 ALFVLDSWLR PVPAGVAGEL YIAGAGVGVG YWRRAGLTAS RFVACPFGGS GARMYRTGDL
1861 VCWRADGQLE FLGRTDDQVK IRGYRIELGE VATALAELAG VGQAVVIARE DRPGDKRLVG
1921 YATEIAPGAV DPAGLRAQLA QRLPGYLVPA AVVVIDALPL TVNGKLDHRA LPAPEYGDTN
1981 GYRAPAGPVE KTVAGIFARV LGLERVGVDD SFFELGGDSL AAMRVIAAIN TTLNADLPVR
2041 ALLHASSTRG LSQLLGRDAR PTSDPRLVSV HGDNPTEVHA SDLTLDRFID ADTLATAVNL
2101 PGPSPELRTV LLTGATGFLG RYLVLELLRR LDVDGRLICL VRAESDEDAR RRLEKTFDSG
2161 DPELLRHFKE LAADRLEVVA GDKSEPDLGL DQPMWRRLAE TVDLIVDSAA MVNAFPYHEL
2221 FGPNVAGTAE LIRIALTTKL KPFTYVSTAD VGAAIEPSAF TEDADIRVIS PTRTVDGGWA
2281 GGYGTSKWAG EVLLREANDL CALPVAVFRC GMILADTSYA GQLNMSDWVT RMVLSLMATG
2341 IAPRSFYEPD SEGNRQRAHF DGLPVTFVAE AIAVLGARVA GSSLAGFATY HVMNPHDDGI
2401 GLDEYVDWLI EAGYPIRRID DFAEWLQRFE ASLGALPDRQ RRHSVLPMLL ASNSQRLQPL
2461 KPTRGCSAPT DRFRAAVRAA KVGSDKDNPD IPHVSAPTII NYVTNLQLLG LL
//