LOCUS AFM05335.1 490 aa PRT BCT 31-AUG-2017 DEFINITION Bernardetia litoralis DSM 6794 UDP-N-acetylmuramyl-tripeptide synthetase protein. ACCESSION CP003345-2823 PROTEIN_ID AFM05335.1 SOURCE Bernardetia litoralis DSM 6794 ORGANISM Bernardetia litoralis DSM 6794 Bacteria; Bacteroidota; Cytophagia; Cytophagales; Bernardetiaceae; Bernardetia. REFERENCE 1 (bases 1 to 4919337) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Peters,L., Ovchinnikova,G., Lu,M., Kyrpides,N., Mavromatis,K., Ivanova,N., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Spring,S., Lang,E., Kopitz,M., Brambilla,E., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE The complete genome of Flexibacter litoralis DSM 6794 JOURNAL Unpublished REFERENCE 2 (bases 1 to 4919337) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Peters,L., Ovchinnikova,G., Lu,M., Kyrpides,N., Mavromatis,K., Ivanova,N., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Spring,S., Lang,E., Kopitz,M., Brambilla,E., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE Direct Submission JOURNAL Submitted (05-JUN-2012) US DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4089456 Source DNA and organism available from Hans-Peter Klenk at the German Collection of Microorganisms and Cell Cultures (DSMZ) (hans-peter.klenk@dsmz.de) Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu) David Bruce (microbe@cuba.jgi-psf.org) Whole genome sequencing and draft assembly at JGI-PGF Finishing done by JGI-LANL Annotation by JGI-ORNL and JGI-PGF The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##MIGS-Data-START## investigation_type :: bacteria_archaea project_name :: Flexibacter litoralis DSM 6794 collection_date :: Missing lat_lon :: 32.840 -117.258 depth :: Missing alt_elev :: Missing country :: USA environment :: Marine num_replicons :: 1 ref_biomaterial :: DSM 6794, ATCC 23117, IFO 15988, NBRC 15988 biotic_relationship :: Free living trophic_level :: Missing rel_to_oxygen :: Aerobe isol_growth_condt :: Missing sequencing_meth :: WGS assembly :: Newbler v. 2.3 (pre-release) finishing_strategy :: Finished GOLD Stamp ID :: Gi04020 Type Strain :: Yes Funding Program :: DOE-GEBA 2009 Gene Calling Method :: Prodigal, GenePRIMP Isolation Site :: Sea water aquarium outflow, La Jolla, California, U.S.A. Temperature Range :: Mesophile Gram Staining :: Gram- ##MIGS-Data-END## ##Genome-Assembly-Data-START## Finishing Goal :: Finished Current Finishing Status :: Finished Assembly Method :: Newbler v. 2.3 Genome Coverage :: 30x Sequencing Technology :: 454/Illumina ##Genome-Assembly-Data-END## FEATURES Qualifiers source /organism="Bernardetia litoralis DSM 6794" /mol_type="genomic DNA" /strain="DSM 6794" /isolation_source="sea water aquarium outflow" /culture_collection="DSM:6794" /type_material="type strain of Bernardetia litoralis" /db_xref="taxon:880071" /country="USA: La Jolla, CA" /lat_lon="32.840 N 117.258 W" protein /locus_tag="Fleli_2993" /EC_number="6.3.2.13" /note="PFAM: Mur ligase family, glutamate ligase domain; Mur ligase family, catalytic domain; Mur ligase middle domain; TIGRFAM: UDP-N-acetylmuramyl-tripeptide synthetase" /transl_table=11 /db_xref="InterPro:IPR000713" /db_xref="InterPro:IPR004101" /db_xref="InterPro:IPR005761" /db_xref="InterPro:IPR013221" BEGIN 1 MEIIELLKNL KTQSIHGDLE IEINQIQFDS RKIAKNDAYV ALKGTVADGH QFIKQAIQDG 61 AKLIVCEKLP VFLESHITYI QVEDTAQALG KLAGNFYGNP SKKLKLIGVT GTNGKTTVVT 121 ILYKLFKKLG YNVGLISTIH NKIKSRIYPS THTTPNPVEL NRLLAEMVKK RCTHVFMEVS 181 SHAIVQKRIE GLHFKGGVFT NISHDHLDYH GTFSDYIRAK KGFFDILPHT SFALVNQDDK 241 RGLVMLQNCK AKHVTFSMKS MSDYKVRIMD SSFEGMQLQI EEKHNEKING KETWTKLIGN 301 FNAYNLLTVY AVAMLLEEDE DEILTELSNI KGVNGRFQRY TSSLGVHTIV DYAHTPDALE 361 NVLKTISEIK KPHQNLITVV GCGGNRDKTK RPEMAKIAAN YSDWSIFTSD NPRTEKPKAI 421 IKDMLEGVKD VDCSKYQTII LRKEAIERAC SMAQKDDIIL VAGKGHETYQ EINGERFDFD 481 DRVILKESLE //