LOCUS AFM04210.1 493 aa PRT BCT 31-AUG-2017 DEFINITION Bernardetia litoralis DSM 6794 Poly(R)-hydroxyalkanoic acid synthase subunit (PHA_synth_III_E) protein. ACCESSION CP003345-1698 PROTEIN_ID AFM04210.1 SOURCE Bernardetia litoralis DSM 6794 ORGANISM Bernardetia litoralis DSM 6794 Bacteria; Bacteroidota; Cytophagia; Cytophagales; Bernardetiaceae; Bernardetia. REFERENCE 1 (bases 1 to 4919337) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Peters,L., Ovchinnikova,G., Lu,M., Kyrpides,N., Mavromatis,K., Ivanova,N., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Spring,S., Lang,E., Kopitz,M., Brambilla,E., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE The complete genome of Flexibacter litoralis DSM 6794 JOURNAL Unpublished REFERENCE 2 (bases 1 to 4919337) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Peters,L., Ovchinnikova,G., Lu,M., Kyrpides,N., Mavromatis,K., Ivanova,N., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Spring,S., Lang,E., Kopitz,M., Brambilla,E., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE Direct Submission JOURNAL Submitted (05-JUN-2012) US DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4089456 Source DNA and organism available from Hans-Peter Klenk at the German Collection of Microorganisms and Cell Cultures (DSMZ) (hans-peter.klenk@dsmz.de) Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu) David Bruce (microbe@cuba.jgi-psf.org) Whole genome sequencing and draft assembly at JGI-PGF Finishing done by JGI-LANL Annotation by JGI-ORNL and JGI-PGF The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##MIGS-Data-START## investigation_type :: bacteria_archaea project_name :: Flexibacter litoralis DSM 6794 collection_date :: Missing lat_lon :: 32.840 -117.258 depth :: Missing alt_elev :: Missing country :: USA environment :: Marine num_replicons :: 1 ref_biomaterial :: DSM 6794, ATCC 23117, IFO 15988, NBRC 15988 biotic_relationship :: Free living trophic_level :: Missing rel_to_oxygen :: Aerobe isol_growth_condt :: Missing sequencing_meth :: WGS assembly :: Newbler v. 2.3 (pre-release) finishing_strategy :: Finished GOLD Stamp ID :: Gi04020 Type Strain :: Yes Funding Program :: DOE-GEBA 2009 Gene Calling Method :: Prodigal, GenePRIMP Isolation Site :: Sea water aquarium outflow, La Jolla, California, U.S.A. Temperature Range :: Mesophile Gram Staining :: Gram- ##MIGS-Data-END## ##Genome-Assembly-Data-START## Finishing Goal :: Finished Current Finishing Status :: Finished Assembly Method :: Newbler v. 2.3 Genome Coverage :: 30x Sequencing Technology :: 454/Illumina ##Genome-Assembly-Data-END## FEATURES Qualifiers source /organism="Bernardetia litoralis DSM 6794" /mol_type="genomic DNA" /strain="DSM 6794" /isolation_source="sea water aquarium outflow" /culture_collection="DSM:6794" /type_material="type strain of Bernardetia litoralis" /db_xref="taxon:880071" /country="USA: La Jolla, CA" /lat_lon="32.840 N 117.258 W" protein /locus_tag="Fleli_1812" /note="PFAM: Poly(R)-hydroxyalkanoic acid synthase subunit (PHA_synth_III_E)" /transl_table=11 BEGIN 1 MANVMDSTKE MLDFWATTQN KIVENWVGST RQLQEAAKEG NVMEQATDMY KNWYDNQKSI 61 ISEAVEKGKT ISSENMPEQV TQLMEQQSKL SKKWMDTMSE MADHQMKIAQ MTNTNGQGAM 121 NIEENAAKVQ EMMTSSYKTW LDSTTEMMNE SKKMMASNPA FKAMQPMQET FENMTKGAKT 181 YFDAYEMWKP FMQMTENKNF DVQEYFKMMN MGKLQEMTKG MFAQNPSQFG FANFGKMSEQ 241 MNKMVEQYRT TMTNNPMFAP YTEMIEKFSS MMPTDMKNFD MSKMMETMNI QSPQFGKEIY 301 ERMEKFYAPF YKLMPPSKEK ESIKLFSHMQ TILFDYQTKM ADMNAIIGKT VKSSSEEFMT 361 KMFESAKDGK IDSYNEFYMN WLNHLEGNMT DIFKGDEFSK LQGEVLGLQL DLKTNFSKGM 421 EQVLSPYPVV LNSEIDELAK QVHALKARVR ELENNAEATP TAKTSETKKT VAKPTVRKAT 481 TAKTTTTKKA TSK //