LOCUS AFM03331.1 537 aa PRT BCT 31-AUG-2017 DEFINITION Bernardetia litoralis DSM 6794 putative spermidine synthase with an N-terminal membrane domain protein. ACCESSION CP003345-819 PROTEIN_ID AFM03331.1 SOURCE Bernardetia litoralis DSM 6794 ORGANISM Bernardetia litoralis DSM 6794 Bacteria; Bacteroidota; Cytophagia; Cytophagales; Bernardetiaceae; Bernardetia. REFERENCE 1 (bases 1 to 4919337) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Peters,L., Ovchinnikova,G., Lu,M., Kyrpides,N., Mavromatis,K., Ivanova,N., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Spring,S., Lang,E., Kopitz,M., Brambilla,E., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE The complete genome of Flexibacter litoralis DSM 6794 JOURNAL Unpublished REFERENCE 2 (bases 1 to 4919337) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Peters,L., Ovchinnikova,G., Lu,M., Kyrpides,N., Mavromatis,K., Ivanova,N., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Spring,S., Lang,E., Kopitz,M., Brambilla,E., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE Direct Submission JOURNAL Submitted (05-JUN-2012) US DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4089456 Source DNA and organism available from Hans-Peter Klenk at the German Collection of Microorganisms and Cell Cultures (DSMZ) (hans-peter.klenk@dsmz.de) Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu) David Bruce (microbe@cuba.jgi-psf.org) Whole genome sequencing and draft assembly at JGI-PGF Finishing done by JGI-LANL Annotation by JGI-ORNL and JGI-PGF The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##MIGS-Data-START## investigation_type :: bacteria_archaea project_name :: Flexibacter litoralis DSM 6794 collection_date :: Missing lat_lon :: 32.840 -117.258 depth :: Missing alt_elev :: Missing country :: USA environment :: Marine num_replicons :: 1 ref_biomaterial :: DSM 6794, ATCC 23117, IFO 15988, NBRC 15988 biotic_relationship :: Free living trophic_level :: Missing rel_to_oxygen :: Aerobe isol_growth_condt :: Missing sequencing_meth :: WGS assembly :: Newbler v. 2.3 (pre-release) finishing_strategy :: Finished GOLD Stamp ID :: Gi04020 Type Strain :: Yes Funding Program :: DOE-GEBA 2009 Gene Calling Method :: Prodigal, GenePRIMP Isolation Site :: Sea water aquarium outflow, La Jolla, California, U.S.A. Temperature Range :: Mesophile Gram Staining :: Gram- ##MIGS-Data-END## ##Genome-Assembly-Data-START## Finishing Goal :: Finished Current Finishing Status :: Finished Assembly Method :: Newbler v. 2.3 Genome Coverage :: 30x Sequencing Technology :: 454/Illumina ##Genome-Assembly-Data-END## FEATURES Qualifiers source /organism="Bernardetia litoralis DSM 6794" /mol_type="genomic DNA" /strain="DSM 6794" /isolation_source="sea water aquarium outflow" /culture_collection="DSM:6794" /type_material="type strain of Bernardetia litoralis" /db_xref="taxon:880071" /country="USA: La Jolla, CA" /lat_lon="32.840 N 117.258 W" protein /locus_tag="Fleli_0874" /note="PFAM: Spermine/spermidine synthase" /transl_table=11 /db_xref="InterPro:IPR001045" BEGIN 1 MDKNKPSSSS FTSFWTPSRT LKVALFATGL SGIVAEYILS TLATYFLGDS VKQFTLIVSV 61 MLFSMGLGSR LSQFFKKNLI ELFIACELIL SVLVSFCSLL TYGIASVSEW IGIVIYTLSV 121 LIGLLIGFEI PLVTRINENY EELRFNISSV MEKDYYGSLI GGAFFAFIGL PFIGLTYTPF 181 ILGIINFLVG ILVLFTLTSL FAEKWQARLK VISLAILGVI VFGLISAKPV VRYGEQKRYK 241 DKIIFEEQSR YQKIVMTQYK KDYWLYLNSH LQFSTFDEWL YHEPLVHPVM QFATKSESQS 301 KKEGKLDILI LGGGDGCAAR EVLKYDNVNT ITLVDLDPSM TKLGKESPII TKVNKNSLNN 361 ERVTVYNGDA FNFLTDSMVF YDVMIVDFPD PKTIEVNRLY TLEFYQLCYQ HLRPQGIMIT 421 QAGSPYYAAK AFDCINKTMQ RAGFETLPMH NQVLTMGEWG WIVGNKSLKT EDLKKVMQNI 481 QIKDIETKWL NHDAMLQITS FGKNWKNTNN SKEDIEINTI HNPVLYRYYQ DGSWYMY //