LOCUS AFI87581.1 940 aa PRT BCT 27-JUN-2016
DEFINITION Aggregatibacter actinomycetemcomitans D7S-1 isoleucine-
-tRNA ligase protein.
ACCESSION CP003496-1530
PROTEIN_ID AFI87581.1
SOURCE Aggregatibacter actinomycetemcomitans D7S-1
ORGANISM Aggregatibacter actinomycetemcomitans D7S-1
Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
Pasteurellaceae; Aggregatibacter.
REFERENCE 1 (bases 1 to 2308962)
AUTHORS Chen,C., Kittichotirat,W., Chen,W., Downey,J.S., Si,Y. and
Bumgarner,R.
TITLE Genome sequence of naturally competent Aggregatibacter
actinomycetemcomitans serotype a strain D7S-1
JOURNAL J. Bacteriol. 192 (10), 2643-2644 (2010)
PUBMED 20348265
REFERENCE 2 (bases 1 to 2308962)
AUTHORS Kittichotirat,W., Bumgarner,R.E. and Chen,C.
TITLE Evolutionary Divergence of Aggregatibacter actinomycetemcomitans
JOURNAL J. Dent. Res. 95 (1), 94-101 (2016)
PUBMED 26420795
REFERENCE 3 (bases 1 to 2308962)
AUTHORS Chen,C., Kittichotirat,W., Chen,W., Downey,J., Si,Y. and
Baumgarner,R.
TITLE Direct Submission
JOURNAL Submitted (01-MAR-2010) Microbiology, University of Washington, 960
Republican St. Rosen Building, Seattle, WA 98109, USA
REFERENCE 4 (bases 1 to 2308962)
AUTHORS Kittichotirat,W., Bumgarner,R. and Chen,C.
TITLE Direct Submission
JOURNAL Submitted (23-APR-2012) Department of Microbiology, University of
Washington, 960 Republican St. Rosen Building, Seattle, WA 98109,
USA
REFERENCE 5 (bases 1 to 2308962)
AUTHORS Kittichotirat,W., Bumgarner,R. and Chen,C.
TITLE Direct Submission
JOURNAL Submitted (18-JUL-2014) Division of Periodontolology, Herman Ostrow
School of Dentistry, University of Southern Californak, 925 W. 34th
Street, Los Angeles, CA 90089, USA
REMARK Sequence update by submitter
COMMENT On Jun 27, 2016 this sequence version replaced CP003496.1.
Source of DNA for this strain is available from: Casey Chen,
Division of Primary Oral Health Care, USC School of Dentistry, 925
W 34th Street, Los Angeles, CA 90089-0641.
Annotation was added by the NCBI Prokaryotic Genome Annotation
Pipeline (released 2013). Information about the Pipeline can be
found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/
##Genome-Assembly-Data-START##
Assembly Date :: 24-MAY-2014
Assembly Method :: 454 Newbler assembler v. 2.0.00.22
Assembly Name :: AaD7S-1v2.0
Genome Coverage :: 17.58x
Sequencing Technology :: 454 Illumina MiSeq
##Assembly-Data-END##
##Genome-Annotation-Data-START##
Annotation Provider :: NCBI
Annotation Date :: 07/17/2014 13:42:00
Annotation Pipeline :: NCBI Prokaryotic Genome Annotation
Pipeline
Annotation Method :: Best-placed reference protein set;
GeneMarkS+
Annotation Software revision :: 2.6 (rev. 440435)
Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA;
repeat_region
Genes :: 2,197
CDS :: 2,041
Pseudo Genes :: 83
CRISPR Arrays :: 3
rRNAs :: 19 (5S, 16S, 23S)
tRNAs :: 53
ncRNA :: 1
Frameshifted Genes :: 69
##Genome-Annotation-Data-END##
FEATURES Qualifiers
source /organism="Aggregatibacter actinomycetemcomitans D7S-1"
/mol_type="genomic DNA"
/strain="D7S-1"
/db_xref="taxon:694569"
protein /gene="ileS"
/locus_tag="D7S_01854"
/EC_number="6.1.1.5"
/inference="EXISTENCE: similar to AA
sequence:RefSeq:WP_005566863.1"
/note="IleRS; catalyzes the formation of
isoleucyl-tRNA(Ile) from isoleucine and tRNA(Ile); since
isoleucine and other amino acids such as valine are
similar, there are additional editing function in this
enzyme; one is involved in hydrolysis of activated
valine-AMP and the other is involved in deacylation of
mischarged Val-tRNA(Ile); there are two active sites, one
for aminoacylation and one for editing; class-I
aminoacyl-tRNA synthetase family type 1 subfamily; some
organisms carry two different copies of this enzyme;
Derived by automated computational analysis using gene
prediction method: Protein Homology."
/transl_table=11
BEGIN
1 MTDYKNTLNL PETGFPMRGD LAKREPVMLK NWYEKDLYQK IRQATKGKKS FILHDGPPYA
61 NGTIHIGHAV NKILKDIIVK SKTALGFDSP YIPGWDCHGL PIELKVEGLV GKPNEKISAA
121 EFRQKCREYA AEQVEGQKKD FVRLGVLGDW DNPYLTMNFN TEANIIRTLG KVIANGHLYK
181 GSKPVHWCLD CGSSLAEAEV EYEDRVSPSI YVRFPAESAV EIEGKFNAVG KGSGKLSAVI
241 WTTTPWTMPS NRAISVNAEL EYRLVQLGDE RVILAADLVD DVAKAVGVEK SEILGSAKGK
301 GLELSRFLHP FYNFTVPIIL GDHVTTDGGT GLVHTAPDHG LDDFIVGKQY DLPMAGLVAN
361 DGKFISSTEF FAGKGVFEAN PLVIEKLQET GNLLKLDKIK HSYPHCWRHK TPIIFRATPQ
421 WFIGMEVQGL RPQALGEIKQ VRWIPDWGQA RIEKMVENRP DWCISRQRTW GVPMTLFVHK
481 ETEELHPRTL ELLEEVARRV EKAGIQAWWD LDEKELLGAD AETYRKVPDT LDVWFDSGST
541 YSSVVANRPE FEGKAADMYL EGSDQHRGWF MSSLMLSTAT DNKAPYKQVL THGFTVDGQG
601 RKMSKSIGNV VTPQEVMDKF GGDILRLWVA STDYTGEMTV SDEILKRAAD SYRRIRNTAR
661 FLLANLNGFD PQRDAVKPEE MISLDRWAVA CALEAQNEIK DAYDNYQFHA VVQRLMRFCS
721 VEMGSFYLDI IKDRQYTTKA DSLARRSCQT ALWHIAEALV RWMAPILSFT ADEIWGHLPK
781 TSTPRAEFVF TEEFYEGLFG LGENEKLDDA YWQTLIKVRS EVNRVLEIAR NDKVIGAGLE
841 AEVTVYTNDE YRALLEKLGD ELRFVLITSK AEVKPLTDKP ADVAEGELEG IAVSVARSNG
901 EKCPRCWHYS DKIGVNPQHP SLCPRCVENV AGNGEIRHFA
//