LOCUS AEH22882.1 321 aa PRT BCT 17-JUL-2013
DEFINITION Thermodesulfobacterium geofontis OPF15 DNA-directed RNA
polymerase subunit alpha protein.
ACCESSION CP002829-765
PROTEIN_ID AEH22882.1
SOURCE Thermodesulfobacterium geofontis OPF15
ORGANISM Thermodesulfobacterium geofontis OPF15
Bacteria; Thermodesulfobacteria; Thermodesulfobacteriales;
Thermodesulfobacteriaceae; Thermodesulfobacterium.
REFERENCE 1 (bases 1 to 1634377)
AUTHORS Elkins,J.G., Hamilton-Brehm,S.D., Lucas,S., Han,J., Lapidus,A.,
Cheng,J.F., Goodwin,L.A., Pitluck,S., Peters,L., Mikhailova,N.,
Davenport,K.W., Detter,J.C., Han,C.S., Tapia,R., Land,M.L.,
Hauser,L., Kyrpides,N.C., Ivanova,N.N., Pagani,I., Bruce,D.,
Woyke,T. and Cottingham,R.W.
TITLE Complete Genome Sequence of the Hyperthermophilic Sulfate-Reducing
Bacterium Thermodesulfobacterium geofontis OPF15T
JOURNAL Genome Announc 1 (2), E00162-13 (2013)
PUBMED 23580711
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 1634377)
AUTHORS Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S.,
Peters,L., Mikhailova,N., Davenport,K., Detter,J.C., Han,C.,
Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I.,
Hamilton-Brehm,S., Elkins,J. and Woyke,T.
CONSRTM US DOE Joint Genome Institute
TITLE Direct Submission
JOURNAL Submitted (01-JUN-2011) US DOE Joint Genome Institute, 2800
Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA
COMMENT URL -- http://www.jgi.doe.gov
JGI Project ID: 4091115
Source DNA and organism available from James G. Elkins
(elkinsjg@ornl.gov)
Contacts: James G. Elkins (elkinsjg@ornl.gov)
Tanja Woyke (microbe@cuba.jgi-psf.org)
Annotation done by JGI-ORNL and JGI-PGF
Finishing done by JGI-LANL
The JGI and collaborators endorse the principles for the
distribution and use of large scale sequencing data adopted by the
larger genome sequencing community and urge users of this data to
follow them. it is our intention to publish the work of this
project in a timely fashion and we welcome collaborative
interaction on the project and analysis.
(http://www.genome.gov/page.cfm?pageID=10506376).
##MIGS-Data-START##
investigation_type :: bacteria_archaea
project_name :: Thermodesulfobacterium geofontis OPF15(T)
collection_date :: 21-Jul-2001
lat_lon :: 44.469 -110.639
depth :: Missing
alt_elev :: approx. 2280 m.
country :: USA
environment :: Fresh water hotspring
num_replicons :: 1
ref_biomaterial :: doi:10.1007/s00792-013-0512-1
biotic_relationship :: Free living
trophic_level :: Chemoautotroph
rel_to_oxygen :: Anaerobe
isol_growth_condt :: doi:10.1007/s00792-013-0512-1
sequencing_meth :: WGS
assembly :: Newbler v. 2.3 (pre-release)
finishing_strategy :: Finished
GOLD Stamp ID :: Gi09586
Funding Program :: DOE-BESC2010
Isolation Site :: Obsidian Pool, Yellowstone National Park,
Wyoming, USA
Isolation Comments :: isolated by James G. Elkins
Cell Shape :: Rod-shaped
Temperature Range :: 60-90C
Temperature Optimum :: 83C
Gram Staining :: Gram-
Diseases :: None
##MIGS-Data-END##
##Genome-Assembly-Data-START##
Finishing Goal :: Finished
Current Finishing Status :: Finished
Assembly Method :: Newbler v. 2.3
Genome Coverage :: 30x
Sequencing Technology :: 454/Illumina
##Genome-Assembly-Data-END##
FEATURES Qualifiers
source /organism="Thermodesulfobacterium geofontis OPF15"
/mol_type="genomic DNA"
/strain="OPF15"
/isolation_source="Obsidian Pool, Yellowstone National
Park"
/type_material="type strain of Thermodesulfobacterium
geofontis"
/db_xref="taxon:795359"
/country="USA: Wyoming"
/lat_lon="44.469 N 110.639 W"
/altitude="2280 m"
/collection_date="21-Jul-2001"
/collected_by="James G. Elkins"
protein /locus_tag="TOPB45_0781"
/inference="protein motif:HAMAP:MF_00059"
/note="SMART: DNA-directed RNA polymerase,
RpoA/D/Rpb3-type; TIGRFAM: DNA-directed RNA polymerase,
alpha subunit; KEGG: csc:Csac_2259 DNA-directed RNA
polymerase subunit alpha; HAMAP: DNA-directed RNA
polymerase, alpha subunit; PFAM: DNA-directed RNA
polymerase, insert; DNA-directed RNA polymerase,
dimerisation; RNA polymerase, alpha subunit, C-terminal"
/transl_table=11
/db_xref="InterPro:IPR011260"
/db_xref="InterPro:IPR011261"
/db_xref="InterPro:IPR011262"
/db_xref="InterPro:IPR011263"
/db_xref="InterPro:IPR011773"
BEGIN
1 MKKEVELIKP EEIKVHKDSQ FPYYGKFIIE PLERGYGITL GNALRRILLS SIPGYAITEV
61 KIEGAPHEFV SLPGIIEDVP EIILNLKGVR FKLNGEGPFY FKLEKVGEGE VKAGDIKINK
121 EGEIVNPDHH IATLTKDGKL FMELKVEKGR GYVPAEYTKN NEIGVILVDA IFSPITKVNF
181 TVGQARVGKS SDYDSLIMEI YTDGTIDPKE ALTQAAKILV EQLMIFLKGE KISLEPYISE
241 APTIENLLNM TIEELDLSGR AYNCLKNAGI NYVGQLVQKT ETELLKLRSF GKKSLDEIIE
301 KLSKLNLRLG MTDIKWKPSK D
//