LOCUS       AEH22379.1               778 aa    PRT              BCT 17-JUL-2013
DEFINITION  Thermodesulfobacterium geofontis OPF15 cysteine synthase protein.
ACCESSION   CP002829-262
PROTEIN_ID  AEH22379.1
SOURCE      Thermodesulfobacterium geofontis OPF15
  ORGANISM  Thermodesulfobacterium geofontis OPF15
            Bacteria; Thermodesulfobacteria; Thermodesulfobacteriales;
            Thermodesulfobacteriaceae; Thermodesulfobacterium.
REFERENCE   1  (bases 1 to 1634377)
  AUTHORS   Elkins,J.G., Hamilton-Brehm,S.D., Lucas,S., Han,J., Lapidus,A.,
            Cheng,J.F., Goodwin,L.A., Pitluck,S., Peters,L., Mikhailova,N.,
            Davenport,K.W., Detter,J.C., Han,C.S., Tapia,R., Land,M.L.,
            Hauser,L., Kyrpides,N.C., Ivanova,N.N., Pagani,I., Bruce,D.,
            Woyke,T. and Cottingham,R.W.
  TITLE     Complete Genome Sequence of the Hyperthermophilic Sulfate-Reducing
            Bacterium Thermodesulfobacterium geofontis OPF15T
  JOURNAL   Genome Announc 1 (2), E00162-13 (2013)
   PUBMED   23580711
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634377)
  AUTHORS   Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S.,
            Peters,L., Mikhailova,N., Davenport,K., Detter,J.C., Han,C.,
            Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I.,
            Hamilton-Brehm,S., Elkins,J. and Woyke,T.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2011) US DOE Joint Genome Institute, 2800
            Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA
COMMENT     URL -- http://www.jgi.doe.gov
            JGI Project ID: 4091115
            Source DNA and organism available from James G. Elkins
            (elkinsjg@ornl.gov)
            Contacts: James G. Elkins (elkinsjg@ornl.gov)
                      Tanja Woyke (microbe@cuba.jgi-psf.org)
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LANL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. it is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            
            ##MIGS-Data-START##
            investigation_type  :: bacteria_archaea
            project_name        :: Thermodesulfobacterium geofontis OPF15(T)
            collection_date     :: 21-Jul-2001
            lat_lon             :: 44.469 -110.639
            depth               :: Missing
            alt_elev            :: approx. 2280 m.
            country             :: USA
            environment         :: Fresh water hotspring
            num_replicons       :: 1
            ref_biomaterial     :: doi:10.1007/s00792-013-0512-1
            biotic_relationship :: Free living
            trophic_level       :: Chemoautotroph
            rel_to_oxygen       :: Anaerobe
            isol_growth_condt   :: doi:10.1007/s00792-013-0512-1
            sequencing_meth     :: WGS
            assembly            :: Newbler v. 2.3 (pre-release)
            finishing_strategy  :: Finished
            GOLD Stamp ID       :: Gi09586
            Funding Program     :: DOE-BESC2010
            Isolation Site      :: Obsidian Pool, Yellowstone National Park,
                                   Wyoming, USA
            Isolation Comments  :: isolated by James G. Elkins
            Cell Shape          :: Rod-shaped
            Temperature Range   :: 60-90C
            Temperature Optimum :: 83C
            Gram Staining       :: Gram-
            Diseases            :: None
            ##MIGS-Data-END##
            
            ##Genome-Assembly-Data-START##
            Finishing Goal           :: Finished
            Current Finishing Status :: Finished
            Assembly Method          :: Newbler v. 2.3
            Genome Coverage          :: 30x
            Sequencing Technology    :: 454/Illumina
            ##Genome-Assembly-Data-END##
FEATURES             Qualifiers
     source          /organism="Thermodesulfobacterium geofontis OPF15"
                     /mol_type="genomic DNA"
                     /strain="OPF15"
                     /isolation_source="Obsidian Pool, Yellowstone National
                     Park"
                     /type_material="type strain of Thermodesulfobacterium
                     geofontis"
                     /db_xref="taxon:795359"
                     /country="USA: Wyoming"
                     /lat_lon="44.469 N 110.639 W"
                     /altitude="2280 m"
                     /collection_date="21-Jul-2001"
                     /collected_by="James G. Elkins"
     protein         /locus_tag="TOPB45_0265"
                     /EC_number="6.1.1.16"
                     /EC_number="2.5.1.47"
                     /inference="protein motif:TFAM:TIGR01136"
                     /note="TIGRFAM: Cysteine synthase K/M; Cysteinyl-tRNA
                     synthetase, class Ia; KEGG: dpr:Despr_3183 cysteine
                     synthase; PFAM: Pyridoxal phosphate-dependent enzyme, beta
                     subunit; Cysteinyl-tRNA synthetase, class Ia, N-terminal"
                     /transl_table=11
                     /db_xref="GO:0004124"
                     /db_xref="GO:0004817"
                     /db_xref="GO:0005524"
                     /db_xref="InterPro:IPR001926"
                     /db_xref="InterPro:IPR002308"
                     /db_xref="InterPro:IPR005856"
                     /db_xref="InterPro:IPR015803"
BEGIN
        1 MRFHNILQKI GNTPIIKLRN IKVKPSIEIW GKLESQNPGG SIKDRPALFM IEEAEKKGLL
       61 TKDKIVIEAS SGNTGIGLSL VCAVKGYKCI IAMPESASIE RRKIMQAYGA EILLTPAEKG
      121 TDGAIETVYE IWRNNPDKYY CTDQFNNDAN WLSHYKTTAP EILRDTEGKV THVVCGLGTT
      181 GTAMGIARFV KDNNLPVKVI GVEPNPGHKI QGLKNMKESY PPSIYKKSLL EKVINIDDEE
      241 AFYWARKLAK EEGIFVGMSS GAALAGALKL AETLDEGLIV VIFPDGGERY LSTPLWSFET
      301 IRKELPKEVD LFLTNTFSGK REAFYPLIDK EVKIYTCGPT LNTRPHIGLY RRLITVDILK
      361 SFLKLMGYKV IHVVNLTDFD DKTINTALEK DVDLRELTSK VEQEFYEDLD WLKIEKADFY
      421 PKVSEHLEDM KKLAISILEK DKAYVKYSSL YFDISRFSEY GKLSKIDLSF LKPGATIDLE
      481 EYDKEEPFDF ALLKRVQILE LKRGYFVETP FGKVRPTWHI HCASLVLKYL GEEIDIFTSG
      541 NDLIFPHHEN TRAIAKALTG KELAKYWVHS GLVFYNGKKI SSENRITIED IKNKGFSGRV
      601 LRFYFLRTHY RNRFNFSWKG LEESSKILEK IDRYIAYLGA TPENEKIEDK DKLWQILKDF
      661 ENNWKEALKE DLNTPIVISE IISFFKKIYP YLKQGLPLDY KKEVLKSLKN LNKILKIFKF
      721 PQVVEKRDIL EKIEIREKAK KEKNYELADR IREELEKEGF FIFDFSFGTR VVSLKEEL
//