LOCUS AEH22379.1 778 aa PRT BCT 17-JUL-2013 DEFINITION Thermodesulfobacterium geofontis OPF15 cysteine synthase protein. ACCESSION CP002829-262 PROTEIN_ID AEH22379.1 SOURCE Thermodesulfobacterium geofontis OPF15 ORGANISM Thermodesulfobacterium geofontis OPF15 Bacteria; Thermodesulfobacteria; Thermodesulfobacteriales; Thermodesulfobacteriaceae; Thermodesulfobacterium. REFERENCE 1 (bases 1 to 1634377) AUTHORS Elkins,J.G., Hamilton-Brehm,S.D., Lucas,S., Han,J., Lapidus,A., Cheng,J.F., Goodwin,L.A., Pitluck,S., Peters,L., Mikhailova,N., Davenport,K.W., Detter,J.C., Han,C.S., Tapia,R., Land,M.L., Hauser,L., Kyrpides,N.C., Ivanova,N.N., Pagani,I., Bruce,D., Woyke,T. and Cottingham,R.W. TITLE Complete Genome Sequence of the Hyperthermophilic Sulfate-Reducing Bacterium Thermodesulfobacterium geofontis OPF15T JOURNAL Genome Announc 1 (2), E00162-13 (2013) PUBMED 23580711 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 1634377) AUTHORS Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Peters,L., Mikhailova,N., Davenport,K., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Hamilton-Brehm,S., Elkins,J. and Woyke,T. CONSRTM US DOE Joint Genome Institute TITLE Direct Submission JOURNAL Submitted (01-JUN-2011) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4091115 Source DNA and organism available from James G. Elkins (elkinsjg@ornl.gov) Contacts: James G. Elkins (elkinsjg@ornl.gov) Tanja Woyke (microbe@cuba.jgi-psf.org) Annotation done by JGI-ORNL and JGI-PGF Finishing done by JGI-LANL The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. it is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##MIGS-Data-START## investigation_type :: bacteria_archaea project_name :: Thermodesulfobacterium geofontis OPF15(T) collection_date :: 21-Jul-2001 lat_lon :: 44.469 -110.639 depth :: Missing alt_elev :: approx. 2280 m. country :: USA environment :: Fresh water hotspring num_replicons :: 1 ref_biomaterial :: doi:10.1007/s00792-013-0512-1 biotic_relationship :: Free living trophic_level :: Chemoautotroph rel_to_oxygen :: Anaerobe isol_growth_condt :: doi:10.1007/s00792-013-0512-1 sequencing_meth :: WGS assembly :: Newbler v. 2.3 (pre-release) finishing_strategy :: Finished GOLD Stamp ID :: Gi09586 Funding Program :: DOE-BESC2010 Isolation Site :: Obsidian Pool, Yellowstone National Park, Wyoming, USA Isolation Comments :: isolated by James G. Elkins Cell Shape :: Rod-shaped Temperature Range :: 60-90C Temperature Optimum :: 83C Gram Staining :: Gram- Diseases :: None ##MIGS-Data-END## ##Genome-Assembly-Data-START## Finishing Goal :: Finished Current Finishing Status :: Finished Assembly Method :: Newbler v. 2.3 Genome Coverage :: 30x Sequencing Technology :: 454/Illumina ##Genome-Assembly-Data-END## FEATURES Qualifiers source /organism="Thermodesulfobacterium geofontis OPF15" /mol_type="genomic DNA" /strain="OPF15" /isolation_source="Obsidian Pool, Yellowstone National Park" /type_material="type strain of Thermodesulfobacterium geofontis" /db_xref="taxon:795359" /country="USA: Wyoming" /lat_lon="44.469 N 110.639 W" /altitude="2280 m" /collection_date="21-Jul-2001" /collected_by="James G. Elkins" protein /locus_tag="TOPB45_0265" /EC_number="6.1.1.16" /EC_number="2.5.1.47" /inference="protein motif:TFAM:TIGR01136" /note="TIGRFAM: Cysteine synthase K/M; Cysteinyl-tRNA synthetase, class Ia; KEGG: dpr:Despr_3183 cysteine synthase; PFAM: Pyridoxal phosphate-dependent enzyme, beta subunit; Cysteinyl-tRNA synthetase, class Ia, N-terminal" /transl_table=11 /db_xref="GO:0004124" /db_xref="GO:0004817" /db_xref="GO:0005524" /db_xref="InterPro:IPR001926" /db_xref="InterPro:IPR002308" /db_xref="InterPro:IPR005856" /db_xref="InterPro:IPR015803" BEGIN 1 MRFHNILQKI GNTPIIKLRN IKVKPSIEIW GKLESQNPGG SIKDRPALFM IEEAEKKGLL 61 TKDKIVIEAS SGNTGIGLSL VCAVKGYKCI IAMPESASIE RRKIMQAYGA EILLTPAEKG 121 TDGAIETVYE IWRNNPDKYY CTDQFNNDAN WLSHYKTTAP EILRDTEGKV THVVCGLGTT 181 GTAMGIARFV KDNNLPVKVI GVEPNPGHKI QGLKNMKESY PPSIYKKSLL EKVINIDDEE 241 AFYWARKLAK EEGIFVGMSS GAALAGALKL AETLDEGLIV VIFPDGGERY LSTPLWSFET 301 IRKELPKEVD LFLTNTFSGK REAFYPLIDK EVKIYTCGPT LNTRPHIGLY RRLITVDILK 361 SFLKLMGYKV IHVVNLTDFD DKTINTALEK DVDLRELTSK VEQEFYEDLD WLKIEKADFY 421 PKVSEHLEDM KKLAISILEK DKAYVKYSSL YFDISRFSEY GKLSKIDLSF LKPGATIDLE 481 EYDKEEPFDF ALLKRVQILE LKRGYFVETP FGKVRPTWHI HCASLVLKYL GEEIDIFTSG 541 NDLIFPHHEN TRAIAKALTG KELAKYWVHS GLVFYNGKKI SSENRITIED IKNKGFSGRV 601 LRFYFLRTHY RNRFNFSWKG LEESSKILEK IDRYIAYLGA TPENEKIEDK DKLWQILKDF 661 ENNWKEALKE DLNTPIVISE IISFFKKIYP YLKQGLPLDY KKEVLKSLKN LNKILKIFKF 721 PQVVEKRDIL EKIEIREKAK KEKNYELADR IREELEKEGF FIFDFSFGTR VVSLKEEL //