LOCUS       AEG75266.1               177 aa    PRT              SYN 24-JAN-2013
DEFINITION  Expression vector pLoxGentrc gentamycin acetyltransferase protein.
ACCESSION   JF934954-1
PROTEIN_ID  AEG75266.1
SOURCE      Expression vector pLoxGentrc
  ORGANISM  Expression vector pLoxGentrc
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 1632; 3874 to 6295)
  AUTHORS   Palmeros,B., Wild,J., Szybalski,W., Le Borgne,S.,
            Hernandez-Chavez,G., Gosset,G., Valle,F. and Bolivar,F.
  TITLE     A family of removable cassettes designed to obtain
            antibiotic-resistance-free genomic modifications of Escherichia
            coli and other bacteria
  JOURNAL   Gene 247 (1-2), 255-264 (2000)
   PUBMED   10773465
REFERENCE   2  (bases 1 to 6295)
  AUTHORS   Sabido,A., Martinez,L.M., de Anda,R., Martinez,A., Bolivar,F. and
            Gosset,G.
  TITLE     A novel plasmid vector designed for chromosomal gene integration
            and expression: Use for developing a genetically stable Escherichia
            coli melanin production strain
  JOURNAL   Plasmid 69 (1), 16-23 (2013)
   PUBMED   22884755
REFERENCE   3  (bases 1 to 6295)
  AUTHORS   Sabido,A. and Gosset,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2011) Cell Engineering and Biocatalysis,
            Biotechnology Institute, Av. Universidad 2001, Cuernavaca, Morelos
            62210, Mexico
FEATURES             Qualifiers
     source          /organism="Expression vector pLoxGentrc"
                     /mol_type="other DNA"
                     /db_xref="taxon:1031331"
                     /lab_host="Escherichia coli"
                     /note="derivative of vector pLoxGen4; numbered such that
                     the first nucleotide is the last nucleotide of intergenic
                     region of M13 bacteriophage and the count increases
                     clockwise; carries ampicillin (bla) and gentamycin (aacC1)
                     resistance genes; two parallel loxP sites from pSL1180 are
                     flanking the aacC1 gene; contains all sequences for the
                     correct expression of a gene from plasmid pTrc99A; carries
                     a ColE1 and M13 bacteriophage replication origins; used
                     for the integration of genes into Escherichia coli"
     protein         /gene="aacC1"
                     /function="confers resistance to gentamycin"
                     /transl_table=11
BEGIN
        1 MLRSSNDVTQ QGSRPKTKLG GSSMGIIRTC RLGPDQVKSM RAALDLFGRE FGDVATYSQH
       61 QPDSDYLGNL LRSKTFIALA AFDQEAVVGA LAAYVLPRFE QPRSEIYIYD LAVSGEHRRQ
      121 GIATALINLL KHEANALGAY VIYVQADYGD DPAVALYTKL GIREEVMHFD IDPSTAT
//