LOCUS AEG75266.1 177 aa PRT SYN 24-JAN-2013 DEFINITION Expression vector pLoxGentrc gentamycin acetyltransferase protein. ACCESSION JF934954-1 PROTEIN_ID AEG75266.1 SOURCE Expression vector pLoxGentrc ORGANISM Expression vector pLoxGentrc other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 1632; 3874 to 6295) AUTHORS Palmeros,B., Wild,J., Szybalski,W., Le Borgne,S., Hernandez-Chavez,G., Gosset,G., Valle,F. and Bolivar,F. TITLE A family of removable cassettes designed to obtain antibiotic-resistance-free genomic modifications of Escherichia coli and other bacteria JOURNAL Gene 247 (1-2), 255-264 (2000) PUBMED 10773465 REFERENCE 2 (bases 1 to 6295) AUTHORS Sabido,A., Martinez,L.M., de Anda,R., Martinez,A., Bolivar,F. and Gosset,G. TITLE A novel plasmid vector designed for chromosomal gene integration and expression: Use for developing a genetically stable Escherichia coli melanin production strain JOURNAL Plasmid 69 (1), 16-23 (2013) PUBMED 22884755 REFERENCE 3 (bases 1 to 6295) AUTHORS Sabido,A. and Gosset,G. TITLE Direct Submission JOURNAL Submitted (11-MAY-2011) Cell Engineering and Biocatalysis, Biotechnology Institute, Av. Universidad 2001, Cuernavaca, Morelos 62210, Mexico FEATURES Qualifiers source /organism="Expression vector pLoxGentrc" /mol_type="other DNA" /db_xref="taxon:1031331" /lab_host="Escherichia coli" /note="derivative of vector pLoxGen4; numbered such that the first nucleotide is the last nucleotide of intergenic region of M13 bacteriophage and the count increases clockwise; carries ampicillin (bla) and gentamycin (aacC1) resistance genes; two parallel loxP sites from pSL1180 are flanking the aacC1 gene; contains all sequences for the correct expression of a gene from plasmid pTrc99A; carries a ColE1 and M13 bacteriophage replication origins; used for the integration of genes into Escherichia coli" protein /gene="aacC1" /function="confers resistance to gentamycin" /transl_table=11 BEGIN 1 MLRSSNDVTQ QGSRPKTKLG GSSMGIIRTC RLGPDQVKSM RAALDLFGRE FGDVATYSQH 61 QPDSDYLGNL LRSKTFIALA AFDQEAVVGA LAAYVLPRFE QPRSEIYIYD LAVSGEHRRQ 121 GIATALINLL KHEANALGAY VIYVQADYGD DPAVALYTKL GIREEVMHFD IDPSTAT //