LOCUS       ADU48889.1               715 aa    PRT              BCT 08-JAN-2015
DEFINITION  Intrasporangium calvum DSM 43043 DNA ligase, NAD-dependent protein.
ACCESSION   CP002343-2327
PROTEIN_ID  ADU48889.1
SOURCE      Intrasporangium calvum DSM 43043
  ORGANISM  Intrasporangium calvum DSM 43043
            Bacteria; Actinobacteria; Micrococcales; Intrasporangiaceae;
            Intrasporangium.
REFERENCE   1  (bases 1 to 4024382)
  AUTHORS   Del Rio,T.G., Chertkov,O., Yasawong,M., Lucas,S., Deshpande,S.,
            Cheng,J.F., Detter,C., Tapia,R., Han,C., Goodwin,L., Pitluck,S.,
            Liolios,K., Ivanova,N., Mavromatis,K., Pati,A., Chen,A.,
            Palaniappan,K., Land,M., Hauser,L., Chang,Y.J., Jeffries,C.D.,
            Rohde,M., Pukall,R., Sikorski,J., Goker,M., Woyke,T., Bristow,J.,
            Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C., Klenk,H.P.
            and Lapidus,A.
  TITLE     Complete genome sequence of Intrasporangium calvum type strain (7
            KIP)
  JOURNAL   Stand Genomic Sci 3 (3), 294-303 (2010)
   PUBMED   21304734
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024382)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Bruce,D.,
            Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N.,
            Chertkov,O., Brettin,T., Tapia,R., Detter,J.C., Han,C., Land,M.,
            Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T.,
            Wu,D., Pukall,R., Gehrich-Schroeter,G., Schneider,S., Klenk,H.-P.
            and Eisen,J.A.
  CONSRTM   US DOE Joint Genome Institute (JGI-PGF)
  TITLE     The complete genome of Intrasporangium calvum DSM 43043
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4024382)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Bruce,D.,
            Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N.,
            Chertkov,O., Brettin,T., Detter,J.C., Tapia,R., Han,C., Land,M.,
            Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T.,
            Wu,D., Pukall,R., Gehrich-Schroeter,G., Schneider,S., Klenk,H.-P.
            and Eisen,J.A.
  CONSRTM   US DOE Joint Genome Institute (JGI-PGF)
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2010) US DOE Joint Genome Institute, 2800
            Mitchell Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     URL -- http://www.jgi.doe.gov
            JGI Project ID: 4083316
            Source DNA and organism available from Hans-Peter Klenk at the
            German Collection of Microorganisms and Cell Cultures (DSMZ)
            (hans-peter.klenk@dsmz.de)
            Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu)
                      David Bruce (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Finishing done by JGI-LANL
            Annotation by JGI-ORNL and JGI-PGF
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            
            ##MIGS-Data-START##
            investigation_type  :: bacteria_archaea
            project_name        :: Intrasporangium calvum 7KIP, DSM 43043
            collection_date     :: 1967
            lat_lon             :: 55.753 37.604
            depth               :: Missing
            alt_elev            :: Missing
            country             :: Russia
            environment         :: Air
            num_replicons       :: Missing
            ref_biomaterial     :: DSM 43043, ATCC 23552, CUB 430, IFO 12989,
                                   IMET 7816, JCM 3097, KCC A-0097, NBRC 12989,
                                   NCIB 10167
            biotic_relationship :: Free living
            trophic_level       :: Chemoorganotroph
            rel_to_oxygen       :: Aerobe
            isol_growth_condt   :: Missing
            sequencing_meth     :: WGS
            assembly            :: Newbler v. 2.3 (pre-release)
            finishing_strategy  :: Finished
            GOLD Stamp ID       :: Gi02320
            Type Strain         :: Yes
            Greengenes ID       :: 90541
            Funding Program     :: DOE-GEBA 2007
            Number of Reads     :: 847906 pyrosequencing, 11758818 Illumina
            Gene Calling Method :: Prodigal 1.4, GenePRIMP
            Isolation Site      :: Air in a school dining room
            Isolation Comments  :: isolated by L.V. Kalakoutskii
            Motility            :: Nonmotile
            Sporulation         :: Nonsporulating
            Temperature Range   :: Mesophile
            Temperature Optimum :: 37C
            Gram Staining       :: Gram+
            Diseases            :: None
            ##MIGS-Data-END##
            
            ##Genome-Assembly-Data-START##
            Finishing Goal           :: Finished
            Current Finishing Status :: Finished
            Assembly Method          :: Newbler v. 2.3
            Genome Coverage          :: 30x
            Sequencing Technology    :: 454-FLX, 454-Titanium, Illumina GAii
            ##Genome-Assembly-Data-END##
FEATURES             Qualifiers
     source          /organism="Intrasporangium calvum DSM 43043"
                     /mol_type="genomic DNA"
                     /strain="DSM 43043"
                     /isolation_source="air in a school dining room"
                     /culture_collection="DSM:43043"
                     /type_material="type strain of Intrasporangium calvum"
                     /db_xref="taxon:710696"
                     /country="Russia"
                     /lat_lon="55.753 N 37.604 E"
                     /collection_date="1967"
                     /collected_by="L.V. Kalakoutskii"
     protein         /locus_tag="Intca_2382"
                     /EC_number="6.5.1.2"
                     /note="COGs: COG0272 NAD-dependent DNA ligase (contains
                     BRCT domain type II); InterProIPR001679: IPR013840:
                     IPR001357: IPR018239: IPR 013839: IPR004150: IPR004149:
                     IPR000445; KEGG: kse:Ksed_09780 DNA ligase, NAD-dependent;
                     PFAM: NAD-dependent DNA ligase adenylation; NAD-dependent
                     DNA ligase OB-fold; helix-hairpin-helix motif; BRCT domain
                     protein; SMART: NAD-dependent DNA ligase; BRCT domain
                     protein; SPTR: DNA ligase; TIGRFAM: DNA ligase,
                     NAD-dependent; PFAM: NAD-dependent DNA ligase OB-fold
                     domain; NAD-dependent DNA ligase adenylation domain; BRCA1
                     C Terminus (BRCT) domain; NAD-dependent DNA ligase C4 zinc
                     finger domain; TIGRFAM: DNA ligase, NAD-dependent"
                     /transl_table=11
BEGIN
        1 MSDSTGSQAI EDAIPDEVHH EWADLAERAS AAQFAYHVKD APTISDAEYD GLIRRLNALE
       61 AQWPSLRTPD SPTQKVGASA FATGFETVDH LERMLSLDNA FSFEELRAWA ERVEREVGAG
      121 AHFLTELKID GLAVNLLYEQ GRLVRALTRG DGRTGEDVTN NVRTIEGVPE RLSGDDVPEL
      181 VEIRGEVFFP TAGFADLNAA LVAAGKAPFA NPRNAAAGSL RQKDPKVTAG RPLRLLVHGI
      241 GARRGFDIAR QSEGYAALRS WGLPTSDQVK VLDDIADVEE FVTFHGEHRH DGLHEIDGIV
      301 VKVDEVTLQR QLGATSRAPR WAIAFKYPPE EVNTRLLDIR VNVGRTGRVT PYGVMQPVVV
      361 AGSTVEQATL HNQHEVERKG VLIGDTVVLR KAGDVIPEIV GPVVDLRDGS ERPFVMPTQC
      421 PSCGTQLAPE KEGDKDIRCP NSRSCPSQLR ERVFALASRS ALDIEALGWE GSIALLESGI
      481 LTDEGDLFAP PEDSPEAGAY GVTRERLAAV GLYTRAAKRT DDPAHVVDGR VLSANGEKLL
      541 AELEKAKTQP LWRVLVALSI RHVGPTAARA LATTFGSMDA IRRASIEELA EAEGVGQIIA
      601 ESVTAWFAVD WHAEIVRKWA AARVRMEDEV DASVARTLEG RTVVVTGSLE GFSRDETKEA
      661 ILSRGGKAAG SVSRKTDWVV VGANAGSKAA KAEELGLRVL DEAQFVRLLE TGSPD
//