LOCUS       ADU48833.1               359 aa    PRT              BCT 08-JAN-2015
DEFINITION  Intrasporangium calvum DSM 43043 Cys/Met metabolism pyridoxal-
            phosphate-dependent protein protein.
ACCESSION   CP002343-2271
PROTEIN_ID  ADU48833.1
SOURCE      Intrasporangium calvum DSM 43043
  ORGANISM  Intrasporangium calvum DSM 43043
            Bacteria; Actinobacteria; Micrococcales; Intrasporangiaceae;
            Intrasporangium.
REFERENCE   1  (bases 1 to 4024382)
  AUTHORS   Del Rio,T.G., Chertkov,O., Yasawong,M., Lucas,S., Deshpande,S.,
            Cheng,J.F., Detter,C., Tapia,R., Han,C., Goodwin,L., Pitluck,S.,
            Liolios,K., Ivanova,N., Mavromatis,K., Pati,A., Chen,A.,
            Palaniappan,K., Land,M., Hauser,L., Chang,Y.J., Jeffries,C.D.,
            Rohde,M., Pukall,R., Sikorski,J., Goker,M., Woyke,T., Bristow,J.,
            Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C., Klenk,H.P.
            and Lapidus,A.
  TITLE     Complete genome sequence of Intrasporangium calvum type strain (7
            KIP)
  JOURNAL   Stand Genomic Sci 3 (3), 294-303 (2010)
   PUBMED   21304734
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024382)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Bruce,D.,
            Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N.,
            Chertkov,O., Brettin,T., Tapia,R., Detter,J.C., Han,C., Land,M.,
            Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T.,
            Wu,D., Pukall,R., Gehrich-Schroeter,G., Schneider,S., Klenk,H.-P.
            and Eisen,J.A.
  CONSRTM   US DOE Joint Genome Institute (JGI-PGF)
  TITLE     The complete genome of Intrasporangium calvum DSM 43043
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4024382)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Bruce,D.,
            Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N.,
            Chertkov,O., Brettin,T., Detter,J.C., Tapia,R., Han,C., Land,M.,
            Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T.,
            Wu,D., Pukall,R., Gehrich-Schroeter,G., Schneider,S., Klenk,H.-P.
            and Eisen,J.A.
  CONSRTM   US DOE Joint Genome Institute (JGI-PGF)
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2010) US DOE Joint Genome Institute, 2800
            Mitchell Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     URL -- http://www.jgi.doe.gov
            JGI Project ID: 4083316
            Source DNA and organism available from Hans-Peter Klenk at the
            German Collection of Microorganisms and Cell Cultures (DSMZ)
            (hans-peter.klenk@dsmz.de)
            Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu)
                      David Bruce (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Finishing done by JGI-LANL
            Annotation by JGI-ORNL and JGI-PGF
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            
            ##MIGS-Data-START##
            investigation_type  :: bacteria_archaea
            project_name        :: Intrasporangium calvum 7KIP, DSM 43043
            collection_date     :: 1967
            lat_lon             :: 55.753 37.604
            depth               :: Missing
            alt_elev            :: Missing
            country             :: Russia
            environment         :: Air
            num_replicons       :: Missing
            ref_biomaterial     :: DSM 43043, ATCC 23552, CUB 430, IFO 12989,
                                   IMET 7816, JCM 3097, KCC A-0097, NBRC 12989,
                                   NCIB 10167
            biotic_relationship :: Free living
            trophic_level       :: Chemoorganotroph
            rel_to_oxygen       :: Aerobe
            isol_growth_condt   :: Missing
            sequencing_meth     :: WGS
            assembly            :: Newbler v. 2.3 (pre-release)
            finishing_strategy  :: Finished
            GOLD Stamp ID       :: Gi02320
            Type Strain         :: Yes
            Greengenes ID       :: 90541
            Funding Program     :: DOE-GEBA 2007
            Number of Reads     :: 847906 pyrosequencing, 11758818 Illumina
            Gene Calling Method :: Prodigal 1.4, GenePRIMP
            Isolation Site      :: Air in a school dining room
            Isolation Comments  :: isolated by L.V. Kalakoutskii
            Motility            :: Nonmotile
            Sporulation         :: Nonsporulating
            Temperature Range   :: Mesophile
            Temperature Optimum :: 37C
            Gram Staining       :: Gram+
            Diseases            :: None
            ##MIGS-Data-END##
            
            ##Genome-Assembly-Data-START##
            Finishing Goal           :: Finished
            Current Finishing Status :: Finished
            Assembly Method          :: Newbler v. 2.3
            Genome Coverage          :: 30x
            Sequencing Technology    :: 454-FLX, 454-Titanium, Illumina GAii
            ##Genome-Assembly-Data-END##
FEATURES             Qualifiers
     source          /organism="Intrasporangium calvum DSM 43043"
                     /mol_type="genomic DNA"
                     /strain="DSM 43043"
                     /isolation_source="air in a school dining room"
                     /culture_collection="DSM:43043"
                     /type_material="type strain of Intrasporangium calvum"
                     /db_xref="taxon:710696"
                     /country="Russia"
                     /lat_lon="55.753 N 37.604 E"
                     /collection_date="1967"
                     /collected_by="L.V. Kalakoutskii"
     protein         /locus_tag="Intca_2324"
                     /note="COGs: COG0626 Cystathionine
                     beta-lyase/cystathionine gamma-synthase; InterPro
                     IPR000277; KEGG: nca:Noca_3292 Cys/Met metabolism
                     pyridoxal-phosphate-dependent enzymes; PFAM: Cys/Met
                     metabolism pyridoxal-phosphate-dependent protein; SPTR:
                     Putative L,L-Cystathionine gamma-Lyase; PFAM: Cys/Met
                     metabolism PLP-dependent enzyme"
                     /transl_table=11
BEGIN
        1 MSETPELHVD TILVSAGRPA AEPGAAVNPP VVLSSTFVAD GPVNYARVGN PTWHAFEEAV
       61 GRLEGGRALV HASGMGAISA ALSVVPAGGT VVVPDTTYNG TGDLLRAFEE AGGRVRRLPA
      121 TDTAGFVAAL DGAALVWLES PTNPLMEVID LTTVLGAARA RGVVSVVDNT LPTPLGCQPL
      181 RLGADVVVHS ATKYLAGHSD VLLGVTVTAD DERGRGLGER LHRARTLGGA IAGPMEVYLG
      241 LRGLRTLALR LERASANAAE LARRLHGHPA VERVRFPGSG AMLSIDVVGG AEAAERVCAA
      301 TRVWVHSTSL GGVESQLERR RRHPSEAATV PANLVRLSVG IEHVEDLWAD LDRALGAAL
//