LOCUS ADU48058.1 688 aa PRT BCT 08-JAN-2015
DEFINITION Intrasporangium calvum DSM 43043 translation elongation
factor 2 (EF-2/EF-G) protein.
ACCESSION CP002343-1496
PROTEIN_ID ADU48058.1
SOURCE Intrasporangium calvum DSM 43043
ORGANISM Intrasporangium calvum DSM 43043
Bacteria; Actinobacteria; Micrococcales; Intrasporangiaceae;
Intrasporangium.
REFERENCE 1 (bases 1 to 4024382)
AUTHORS Del Rio,T.G., Chertkov,O., Yasawong,M., Lucas,S., Deshpande,S.,
Cheng,J.F., Detter,C., Tapia,R., Han,C., Goodwin,L., Pitluck,S.,
Liolios,K., Ivanova,N., Mavromatis,K., Pati,A., Chen,A.,
Palaniappan,K., Land,M., Hauser,L., Chang,Y.J., Jeffries,C.D.,
Rohde,M., Pukall,R., Sikorski,J., Goker,M., Woyke,T., Bristow,J.,
Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C., Klenk,H.P.
and Lapidus,A.
TITLE Complete genome sequence of Intrasporangium calvum type strain (7
KIP)
JOURNAL Stand Genomic Sci 3 (3), 294-303 (2010)
PUBMED 21304734
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 4024382)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Bruce,D.,
Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N.,
Chertkov,O., Brettin,T., Tapia,R., Detter,J.C., Han,C., Land,M.,
Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T.,
Wu,D., Pukall,R., Gehrich-Schroeter,G., Schneider,S., Klenk,H.-P.
and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE The complete genome of Intrasporangium calvum DSM 43043
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 4024382)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Bruce,D.,
Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N.,
Chertkov,O., Brettin,T., Detter,J.C., Tapia,R., Han,C., Land,M.,
Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T.,
Wu,D., Pukall,R., Gehrich-Schroeter,G., Schneider,S., Klenk,H.-P.
and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE Direct Submission
JOURNAL Submitted (17-NOV-2010) US DOE Joint Genome Institute, 2800
Mitchell Drive, Walnut Creek, CA 94598-1698, USA
COMMENT URL -- http://www.jgi.doe.gov
JGI Project ID: 4083316
Source DNA and organism available from Hans-Peter Klenk at the
German Collection of Microorganisms and Cell Cultures (DSMZ)
(hans-peter.klenk@dsmz.de)
Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu)
David Bruce (microbe@cuba.jgi-psf.org)
Whole genome sequencing and draft assembly at JGI-PGF
Finishing done by JGI-LANL
Annotation by JGI-ORNL and JGI-PGF
The JGI and collaborators endorse the principles for the
distribution and use of large scale sequencing data adopted by the
larger genome sequencing community and urge users of this data to
follow them. It is our intention to publish the work of this
project in a timely fashion and we welcome collaborative
interaction on the project and analysis.
(http://www.genome.gov/page.cfm?pageID=10506376).
##MIGS-Data-START##
investigation_type :: bacteria_archaea
project_name :: Intrasporangium calvum 7KIP, DSM 43043
collection_date :: 1967
lat_lon :: 55.753 37.604
depth :: Missing
alt_elev :: Missing
country :: Russia
environment :: Air
num_replicons :: Missing
ref_biomaterial :: DSM 43043, ATCC 23552, CUB 430, IFO 12989,
IMET 7816, JCM 3097, KCC A-0097, NBRC 12989,
NCIB 10167
biotic_relationship :: Free living
trophic_level :: Chemoorganotroph
rel_to_oxygen :: Aerobe
isol_growth_condt :: Missing
sequencing_meth :: WGS
assembly :: Newbler v. 2.3 (pre-release)
finishing_strategy :: Finished
GOLD Stamp ID :: Gi02320
Type Strain :: Yes
Greengenes ID :: 90541
Funding Program :: DOE-GEBA 2007
Number of Reads :: 847906 pyrosequencing, 11758818 Illumina
Gene Calling Method :: Prodigal 1.4, GenePRIMP
Isolation Site :: Air in a school dining room
Isolation Comments :: isolated by L.V. Kalakoutskii
Motility :: Nonmotile
Sporulation :: Nonsporulating
Temperature Range :: Mesophile
Temperature Optimum :: 37C
Gram Staining :: Gram+
Diseases :: None
##MIGS-Data-END##
##Genome-Assembly-Data-START##
Finishing Goal :: Finished
Current Finishing Status :: Finished
Assembly Method :: Newbler v. 2.3
Genome Coverage :: 30x
Sequencing Technology :: 454-FLX, 454-Titanium, Illumina GAii
##Genome-Assembly-Data-END##
FEATURES Qualifiers
source /organism="Intrasporangium calvum DSM 43043"
/mol_type="genomic DNA"
/strain="DSM 43043"
/isolation_source="air in a school dining room"
/culture_collection="DSM:43043"
/type_material="type strain of Intrasporangium calvum"
/db_xref="taxon:710696"
/country="Russia"
/lat_lon="55.753 N 37.604 E"
/collection_date="1967"
/collected_by="L.V. Kalakoutskii"
protein /locus_tag="Intca_1545"
/note="COGs: COG0480 Translation elongation factors
(GTPase); InterProIPR005225: IPR000795: IPR004161:
IPR005517: IPR 000640; KEGG: nca:Noca_4599 elongation
factor G; PFAM: elongation factor G domain IV; protein
synthesis factor GTP-binding; elongation factor Tu domain
2 protein; elongation factor G domain-containing protein;
SPTR: Translation elongation factor EF-G; TIGRFAM: small
GTP-binding protein; PFAM: Elongation factor Tu domain 2;
Elongation factor G C-terminus; Elongation factor Tu GTP
binding domain; Elongation factor G, domain IV; TIGRFAM:
translation elongation factor EF-G; small GTP-binding
protein domain"
/transl_table=11
BEGIN
1 MARQTGRSDL PAAPNPASPQ DIRNVVLVGP GGAGKTALFE ALVAARVPAQ RLSTNGHVRT
61 STLTAASFDS NGTTLNLLDT PGYPDFVGEL RAGLRAADAA IFVVSGADEI DRPVSLLWRE
121 CAAVGMPRAV VVTHLDQPRA DFGTTVETCQ RTFGDARATH WPVLEGGELT SVVGLITGSD
181 DDLSTDEHQD ARHALIESVI EESEDATLLD RYLEGESIEP AAILEDLRTA IIRGTFFPIL
241 PVSPMTGAGV DELLSLIEQA FPDPTVHPLP AITSPAGAAI TDVRCDPEAP LVAEVVRTTT
301 DAYVGRLSLV RVFSGTLSVD DTVHVSGHMG DFVGREVSGH PSHDDDERVG PLAAPFLDSH
361 RPRGEAIAGD LVLVSKLTRA ETSDTLSSLD RRAVVAPWIL PEPQLPVAIR AVTKSDEDKL
421 ASALQRLVAE DVTVRMEHAS ETDQVVLWTM GQAHVDDLIS QLSERYGVSV QAEPYRTALR
481 ETFIRKVAVQ GRHVKQSGGH GQYAVCQIEI EPLPRGSGFE FVDKVVGGSV PRQFIPSVEK
541 GARAQLEKGL LTGHPVVDVR VTLVDGKAHS VDSSDVAFQT AAALALKEAA NETTVALLEP
601 IDKVDITIAD EYLGAVMADL RGRRGQVVGT EPAEESGWTV VHAEVPASEL SRYAIDLRSV
661 SHGTGSFVRE PVRHDLIPPD KAKELLKA
//