LOCUS ADU48045.1 1197 aa PRT BCT 08-JAN-2015
DEFINITION Intrasporangium calvum DSM 43043 DNA polymerase III, alpha
subunit protein.
ACCESSION CP002343-1483
PROTEIN_ID ADU48045.1
SOURCE Intrasporangium calvum DSM 43043
ORGANISM Intrasporangium calvum DSM 43043
Bacteria; Actinobacteria; Micrococcales; Intrasporangiaceae;
Intrasporangium.
REFERENCE 1 (bases 1 to 4024382)
AUTHORS Del Rio,T.G., Chertkov,O., Yasawong,M., Lucas,S., Deshpande,S.,
Cheng,J.F., Detter,C., Tapia,R., Han,C., Goodwin,L., Pitluck,S.,
Liolios,K., Ivanova,N., Mavromatis,K., Pati,A., Chen,A.,
Palaniappan,K., Land,M., Hauser,L., Chang,Y.J., Jeffries,C.D.,
Rohde,M., Pukall,R., Sikorski,J., Goker,M., Woyke,T., Bristow,J.,
Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C., Klenk,H.P.
and Lapidus,A.
TITLE Complete genome sequence of Intrasporangium calvum type strain (7
KIP)
JOURNAL Stand Genomic Sci 3 (3), 294-303 (2010)
PUBMED 21304734
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 4024382)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Bruce,D.,
Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N.,
Chertkov,O., Brettin,T., Tapia,R., Detter,J.C., Han,C., Land,M.,
Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T.,
Wu,D., Pukall,R., Gehrich-Schroeter,G., Schneider,S., Klenk,H.-P.
and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE The complete genome of Intrasporangium calvum DSM 43043
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 4024382)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Bruce,D.,
Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N.,
Chertkov,O., Brettin,T., Detter,J.C., Tapia,R., Han,C., Land,M.,
Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T.,
Wu,D., Pukall,R., Gehrich-Schroeter,G., Schneider,S., Klenk,H.-P.
and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE Direct Submission
JOURNAL Submitted (17-NOV-2010) US DOE Joint Genome Institute, 2800
Mitchell Drive, Walnut Creek, CA 94598-1698, USA
COMMENT URL -- http://www.jgi.doe.gov
JGI Project ID: 4083316
Source DNA and organism available from Hans-Peter Klenk at the
German Collection of Microorganisms and Cell Cultures (DSMZ)
(hans-peter.klenk@dsmz.de)
Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu)
David Bruce (microbe@cuba.jgi-psf.org)
Whole genome sequencing and draft assembly at JGI-PGF
Finishing done by JGI-LANL
Annotation by JGI-ORNL and JGI-PGF
The JGI and collaborators endorse the principles for the
distribution and use of large scale sequencing data adopted by the
larger genome sequencing community and urge users of this data to
follow them. It is our intention to publish the work of this
project in a timely fashion and we welcome collaborative
interaction on the project and analysis.
(http://www.genome.gov/page.cfm?pageID=10506376).
##MIGS-Data-START##
investigation_type :: bacteria_archaea
project_name :: Intrasporangium calvum 7KIP, DSM 43043
collection_date :: 1967
lat_lon :: 55.753 37.604
depth :: Missing
alt_elev :: Missing
country :: Russia
environment :: Air
num_replicons :: Missing
ref_biomaterial :: DSM 43043, ATCC 23552, CUB 430, IFO 12989,
IMET 7816, JCM 3097, KCC A-0097, NBRC 12989,
NCIB 10167
biotic_relationship :: Free living
trophic_level :: Chemoorganotroph
rel_to_oxygen :: Aerobe
isol_growth_condt :: Missing
sequencing_meth :: WGS
assembly :: Newbler v. 2.3 (pre-release)
finishing_strategy :: Finished
GOLD Stamp ID :: Gi02320
Type Strain :: Yes
Greengenes ID :: 90541
Funding Program :: DOE-GEBA 2007
Number of Reads :: 847906 pyrosequencing, 11758818 Illumina
Gene Calling Method :: Prodigal 1.4, GenePRIMP
Isolation Site :: Air in a school dining room
Isolation Comments :: isolated by L.V. Kalakoutskii
Motility :: Nonmotile
Sporulation :: Nonsporulating
Temperature Range :: Mesophile
Temperature Optimum :: 37C
Gram Staining :: Gram+
Diseases :: None
##MIGS-Data-END##
##Genome-Assembly-Data-START##
Finishing Goal :: Finished
Current Finishing Status :: Finished
Assembly Method :: Newbler v. 2.3
Genome Coverage :: 30x
Sequencing Technology :: 454-FLX, 454-Titanium, Illumina GAii
##Genome-Assembly-Data-END##
FEATURES Qualifiers
source /organism="Intrasporangium calvum DSM 43043"
/mol_type="genomic DNA"
/strain="DSM 43043"
/isolation_source="air in a school dining room"
/culture_collection="DSM:43043"
/type_material="type strain of Intrasporangium calvum"
/db_xref="taxon:710696"
/country="Russia"
/lat_lon="55.753 N 37.604 E"
/collection_date="1967"
/collected_by="L.V. Kalakoutskii"
protein /locus_tag="Intca_1531"
/EC_number="2.7.7.7"
/note="COGs: COG0587 DNA polymerase III alpha subunit;
InterProIPR004805: IPR004013: IPR011708: IPR004365: IPR
003141; KEGG: kse:Ksed_16450 DNA polymerase III subunit
alpha; PFAM: DNA polymerase III alpha subunit; PHP domain
protein; nucleic acid binding OB-fold tRNA/helicase-type;
PRIAM: DNA-directed DNA polymerase; SMART: phosphoesterase
PHP domain protein; SPTR: DNA polymerase III subunit
alpha; TIGRFAM: DNA polymerase III, alpha subunit; PFAM:
Bacterial DNA polymerase III alpha subunit; PHP domain;
OB-fold nucleic acid binding domain; TIGRFAM: DNA-directed
DNA polymerase III (polc)"
/transl_table=11
BEGIN
1 MSTDPAPRPD QNFVHLHVHT EYSMLDGAAR IGDLFTEAAR MGMPALATTD HGYVFGAYEF
61 WKKAKGTGVK PIIGVEAYIT PGTHRADRTK VSWGDGGRDD VSGSGAYTHM TMLAENNDGM
121 HNLFRMSSIA SLDQVYTKWP RIDRELLSSY GKGLIVTTGC PSSEIQTKLR FGMYDAAREV
181 AGELRDIFGR DNVFVELMDH GLDIERRTQR ELIRLARDLD LPLLATNDLH YTRAEDAKAH
241 AALLCVQSGS TLMDPNRFKF DAEDFYLKSA QEMRHLWREL PEACDNTLLV AERCEVSFTE
301 GEGRFMPRFP VPEGEDETSW FIKEVETGLQ RRFPEGVPDY ARKQAAFEED VIVSKGYSGY
361 FLVVADFINW AKRNGIRVGP GRGSGAGSMC AYAMGITDLD PIPHGLIFER FLNPERMSMP
421 DFDVDFDERR RGEVIKYVTE KYGSERVAQI VTYGTIKAKQ AVKDAARVMG HPFSVGEQLT
481 KAMPPDVMGK GVPLAEIYNP EHKRYNEGSE FRALVQTERH FEEVVDAAKG LEGLKRQWGV
541 HAAGVIMSSE PLIDVIPIMK RLQDGQVITQ FDYPSCEALG LVKMDFLGLR NLTILDDAIE
601 NIKANRDLDI DLDALSKDMT DRATYDLLGR GDTLGVFQLD GGGMRALLRL MQPDNFEDIS
661 AALALYRPGP MGVNAHTNFA LRKNGKQALT PLDPKLEGKL QPEMVAALEP ILGTTYGLVI
721 YQEQVMEIAQ KLAGYTLGNA DLLRRAMGKK KKEVLDAEYV PFSDGMKQKG FNEASVKALW
781 EVLVPFSDYA FNKAHTAAYG LVSYWTGYLK ANYPAEYMAA LLTSVRDDKD KSALYLNECR
841 HMGIKVLPPD VNESVANFAA VGTDIRFGMA AIRNVGVPVV EAVIKAREEK GAFASFKDFL
901 SKVPAVVCNK RTIESLIKAG AFDSLGESRR GMVEVHERYV DALVEVKKQE AIGQDSLFGA
961 FDMGDQSGST GGAGAFDGLP GVPSLEWDKA TLLSFEREML GLYVSDHPLF GIEHILQSHA
1021 DTSIASLTGD DPKPDGTPVT VAGLITSLQL KRTKKGDLWA IATVEDLDGA IECLFFPSAY
1081 LTYSTMLAQD TVCAVKGRIS ARDDAISIFA QELTIPDIKE GPRGPVVLTL PLGQVTAARA
1141 ERLREILREH PGATEVQVKL TQPGRSVLMT LDDSLRVTAT SALFGDLKAQ FGAAVLG
//