LOCUS ADU47818.1 1124 aa PRT BCT 08-JAN-2015
DEFINITION Intrasporangium calvum DSM 43043 pyruvate carboxylase protein.
ACCESSION CP002343-1256
PROTEIN_ID ADU47818.1
SOURCE Intrasporangium calvum DSM 43043
ORGANISM Intrasporangium calvum DSM 43043
Bacteria; Actinobacteria; Micrococcales; Intrasporangiaceae;
Intrasporangium.
REFERENCE 1 (bases 1 to 4024382)
AUTHORS Del Rio,T.G., Chertkov,O., Yasawong,M., Lucas,S., Deshpande,S.,
Cheng,J.F., Detter,C., Tapia,R., Han,C., Goodwin,L., Pitluck,S.,
Liolios,K., Ivanova,N., Mavromatis,K., Pati,A., Chen,A.,
Palaniappan,K., Land,M., Hauser,L., Chang,Y.J., Jeffries,C.D.,
Rohde,M., Pukall,R., Sikorski,J., Goker,M., Woyke,T., Bristow,J.,
Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C., Klenk,H.P.
and Lapidus,A.
TITLE Complete genome sequence of Intrasporangium calvum type strain (7
KIP)
JOURNAL Stand Genomic Sci 3 (3), 294-303 (2010)
PUBMED 21304734
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 4024382)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Bruce,D.,
Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N.,
Chertkov,O., Brettin,T., Tapia,R., Detter,J.C., Han,C., Land,M.,
Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T.,
Wu,D., Pukall,R., Gehrich-Schroeter,G., Schneider,S., Klenk,H.-P.
and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE The complete genome of Intrasporangium calvum DSM 43043
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 4024382)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Bruce,D.,
Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N.,
Chertkov,O., Brettin,T., Detter,J.C., Tapia,R., Han,C., Land,M.,
Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T.,
Wu,D., Pukall,R., Gehrich-Schroeter,G., Schneider,S., Klenk,H.-P.
and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE Direct Submission
JOURNAL Submitted (17-NOV-2010) US DOE Joint Genome Institute, 2800
Mitchell Drive, Walnut Creek, CA 94598-1698, USA
COMMENT URL -- http://www.jgi.doe.gov
JGI Project ID: 4083316
Source DNA and organism available from Hans-Peter Klenk at the
German Collection of Microorganisms and Cell Cultures (DSMZ)
(hans-peter.klenk@dsmz.de)
Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu)
David Bruce (microbe@cuba.jgi-psf.org)
Whole genome sequencing and draft assembly at JGI-PGF
Finishing done by JGI-LANL
Annotation by JGI-ORNL and JGI-PGF
The JGI and collaborators endorse the principles for the
distribution and use of large scale sequencing data adopted by the
larger genome sequencing community and urge users of this data to
follow them. It is our intention to publish the work of this
project in a timely fashion and we welcome collaborative
interaction on the project and analysis.
(http://www.genome.gov/page.cfm?pageID=10506376).
##MIGS-Data-START##
investigation_type :: bacteria_archaea
project_name :: Intrasporangium calvum 7KIP, DSM 43043
collection_date :: 1967
lat_lon :: 55.753 37.604
depth :: Missing
alt_elev :: Missing
country :: Russia
environment :: Air
num_replicons :: Missing
ref_biomaterial :: DSM 43043, ATCC 23552, CUB 430, IFO 12989,
IMET 7816, JCM 3097, KCC A-0097, NBRC 12989,
NCIB 10167
biotic_relationship :: Free living
trophic_level :: Chemoorganotroph
rel_to_oxygen :: Aerobe
isol_growth_condt :: Missing
sequencing_meth :: WGS
assembly :: Newbler v. 2.3 (pre-release)
finishing_strategy :: Finished
GOLD Stamp ID :: Gi02320
Type Strain :: Yes
Greengenes ID :: 90541
Funding Program :: DOE-GEBA 2007
Number of Reads :: 847906 pyrosequencing, 11758818 Illumina
Gene Calling Method :: Prodigal 1.4, GenePRIMP
Isolation Site :: Air in a school dining room
Isolation Comments :: isolated by L.V. Kalakoutskii
Motility :: Nonmotile
Sporulation :: Nonsporulating
Temperature Range :: Mesophile
Temperature Optimum :: 37C
Gram Staining :: Gram+
Diseases :: None
##MIGS-Data-END##
##Genome-Assembly-Data-START##
Finishing Goal :: Finished
Current Finishing Status :: Finished
Assembly Method :: Newbler v. 2.3
Genome Coverage :: 30x
Sequencing Technology :: 454-FLX, 454-Titanium, Illumina GAii
##Genome-Assembly-Data-END##
FEATURES Qualifiers
source /organism="Intrasporangium calvum DSM 43043"
/mol_type="genomic DNA"
/strain="DSM 43043"
/isolation_source="air in a school dining room"
/culture_collection="DSM:43043"
/type_material="type strain of Intrasporangium calvum"
/db_xref="taxon:710696"
/country="Russia"
/lat_lon="55.753 N 37.604 E"
/collection_date="1967"
/collected_by="L.V. Kalakoutskii"
protein /locus_tag="Intca_R0022"
/note="COGs: COG1038 Pyruvate carboxylase;
InterProIPR005930: IPR000634: IPR005479: IPR005481: IPR
005482: IPR000891: IPR003379: IPR000089: IPR011761:
IPR011764; KEGG: gob:Gobs_1751 pyruvate carboxylase; PFAM:
Carbamoyl-phosphate synthase L chain ATP-binding;
Carbamoyl-phosphate synthetase large chain domain protein;
biotin carboxylase domain protein; pyruvate
carboxyltransferase; Conserved carboxylase region;
biotin/lipoyl attachment domain-containing protein; SPTR:
Pyruvate carboxylase; TIGRFAM: pyruvate carboxylase; tmRNA
as predicted by Rfam (RF00023), score 168.01; PFAM:
Carbamoyl-phosphate synthase L chain, ATP binding domain;
Biotin carboxylase C-terminal domain; HMGL-like;
Carbamoyl-phosphate synthase L chain, N-terminal domain;
Biotin-requiring enzyme; Conserved carboxylase domain;
TIGRFAM: pyruvate carboxylase"
/transl_table=11
BEGIN
1 MFRKVLVANR GEIAVRAFRA ATELGARTVA VFPYEDRKSE HRLKADEAYQ IGEEGHPVRS
61 YLDHEAIVAQ AVEAGADAIY PGYGFLSENP LLAEACERAG ITFVGPPPSV LHLTGNKSRA
121 IEAARAAGLP TLHGSSASQD LASLEAAAAE IGFPVFVKAV SGGGGRGMRR VDRPELLAEA
181 LQAAQREAES AFGDPTLYLE EAVVNPRHIE VQVLADGTGE VIHLFERDCS VQRRHQKVIE
241 IAPAPNLDPQ VREQMCADAV AFARSIGYRN AGTVEFLLGE DGRYVFIEMN PRIQVEHTVT
301 EEVTDVDLVV TQMQIASGET LESLGLRQDD IRTRGFAMQC RITTEDPANG FRPDAGTITT
361 YRSAGGSGVR LDGGTVFVGA EVSAHFDSML VKLTCRGRTF PMAVRRAQRA LAEFRIRGVS
421 TNIPFLRAVL ADPTFQAGRA TTSFIDERPE LLTAKVSADR ATRLLTYLAD VTVNQPHGPA
481 TTRVRPRAKL PALPDAPLPR GSRDLLLEVG PAEFARRLRE QTQVAVTDTT FRDAHQSLLA
541 TRMRSRDLLH VAGHVAHLTP QLLSMEAWGG ATYDVALRFL AEDPWERLAA LREAMPNIPL
601 QMLLRGRNTV GYTPYPTAVT DAFVHEAART GLDIFRIFDS LNDVSQMRPA VEAVLETGTA
661 VAEVALCYTG DLLSPSETLY TLDYYLRLAE QIVDTGAHVL AIKDMAGLLR APAAARLVTA
721 LRERFDLPVH LHTHDTAGGQ VATLLAAIGA GVDAVDAATA TMAGTTSQPS LSALVAATDH
781 TERPTGLDID RVFSLEPYWE AVRHLYRPFE SGLESPTGRV YFHEIPGGQL SNLRQQAIAL
841 GLGDRFEAIE DTYAAANRIL GNIVKVTPSS KVVGDLALAL VGAGADPADF EADPQKYDIP
901 DSVIGFLNGE LGDPPGGWPE PFRTKALAGR QARPRLTQLT AEQEQALATE PRRTLNQLLF
961 PGPTKEFEAS REAYGDLSVL GTVEFLHGLV QGEEYEVDIE QGKRLIIGVQ SVGDADSKGM
1021 RTVMCTLGGQ FRPIQVRDRS IEADVKSAEK ADPAAPGQVA APFAGVVTLT VAVGDTVEAG
1081 GVVATIEAMK MEANITSAAG GTVSRVAIGA QQQVEGGDLL VVIT
//