LOCUS ADU47416.1 860 aa PRT BCT 08-JAN-2015
DEFINITION Intrasporangium calvum DSM 43043 peptidase S45 penicillin
amidase protein.
ACCESSION CP002343-854
PROTEIN_ID ADU47416.1
SOURCE Intrasporangium calvum DSM 43043
ORGANISM Intrasporangium calvum DSM 43043
Bacteria; Actinobacteria; Micrococcales; Intrasporangiaceae;
Intrasporangium.
REFERENCE 1 (bases 1 to 4024382)
AUTHORS Del Rio,T.G., Chertkov,O., Yasawong,M., Lucas,S., Deshpande,S.,
Cheng,J.F., Detter,C., Tapia,R., Han,C., Goodwin,L., Pitluck,S.,
Liolios,K., Ivanova,N., Mavromatis,K., Pati,A., Chen,A.,
Palaniappan,K., Land,M., Hauser,L., Chang,Y.J., Jeffries,C.D.,
Rohde,M., Pukall,R., Sikorski,J., Goker,M., Woyke,T., Bristow,J.,
Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C., Klenk,H.P.
and Lapidus,A.
TITLE Complete genome sequence of Intrasporangium calvum type strain (7
KIP)
JOURNAL Stand Genomic Sci 3 (3), 294-303 (2010)
PUBMED 21304734
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 4024382)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Bruce,D.,
Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N.,
Chertkov,O., Brettin,T., Tapia,R., Detter,J.C., Han,C., Land,M.,
Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T.,
Wu,D., Pukall,R., Gehrich-Schroeter,G., Schneider,S., Klenk,H.-P.
and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE The complete genome of Intrasporangium calvum DSM 43043
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 4024382)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Bruce,D.,
Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N.,
Chertkov,O., Brettin,T., Detter,J.C., Tapia,R., Han,C., Land,M.,
Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T.,
Wu,D., Pukall,R., Gehrich-Schroeter,G., Schneider,S., Klenk,H.-P.
and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE Direct Submission
JOURNAL Submitted (17-NOV-2010) US DOE Joint Genome Institute, 2800
Mitchell Drive, Walnut Creek, CA 94598-1698, USA
COMMENT URL -- http://www.jgi.doe.gov
JGI Project ID: 4083316
Source DNA and organism available from Hans-Peter Klenk at the
German Collection of Microorganisms and Cell Cultures (DSMZ)
(hans-peter.klenk@dsmz.de)
Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu)
David Bruce (microbe@cuba.jgi-psf.org)
Whole genome sequencing and draft assembly at JGI-PGF
Finishing done by JGI-LANL
Annotation by JGI-ORNL and JGI-PGF
The JGI and collaborators endorse the principles for the
distribution and use of large scale sequencing data adopted by the
larger genome sequencing community and urge users of this data to
follow them. It is our intention to publish the work of this
project in a timely fashion and we welcome collaborative
interaction on the project and analysis.
(http://www.genome.gov/page.cfm?pageID=10506376).
##MIGS-Data-START##
investigation_type :: bacteria_archaea
project_name :: Intrasporangium calvum 7KIP, DSM 43043
collection_date :: 1967
lat_lon :: 55.753 37.604
depth :: Missing
alt_elev :: Missing
country :: Russia
environment :: Air
num_replicons :: Missing
ref_biomaterial :: DSM 43043, ATCC 23552, CUB 430, IFO 12989,
IMET 7816, JCM 3097, KCC A-0097, NBRC 12989,
NCIB 10167
biotic_relationship :: Free living
trophic_level :: Chemoorganotroph
rel_to_oxygen :: Aerobe
isol_growth_condt :: Missing
sequencing_meth :: WGS
assembly :: Newbler v. 2.3 (pre-release)
finishing_strategy :: Finished
GOLD Stamp ID :: Gi02320
Type Strain :: Yes
Greengenes ID :: 90541
Funding Program :: DOE-GEBA 2007
Number of Reads :: 847906 pyrosequencing, 11758818 Illumina
Gene Calling Method :: Prodigal 1.4, GenePRIMP
Isolation Site :: Air in a school dining room
Isolation Comments :: isolated by L.V. Kalakoutskii
Motility :: Nonmotile
Sporulation :: Nonsporulating
Temperature Range :: Mesophile
Temperature Optimum :: 37C
Gram Staining :: Gram+
Diseases :: None
##MIGS-Data-END##
##Genome-Assembly-Data-START##
Finishing Goal :: Finished
Current Finishing Status :: Finished
Assembly Method :: Newbler v. 2.3
Genome Coverage :: 30x
Sequencing Technology :: 454-FLX, 454-Titanium, Illumina GAii
##Genome-Assembly-Data-END##
FEATURES Qualifiers
source /organism="Intrasporangium calvum DSM 43043"
/mol_type="genomic DNA"
/strain="DSM 43043"
/isolation_source="air in a school dining room"
/culture_collection="DSM:43043"
/type_material="type strain of Intrasporangium calvum"
/db_xref="taxon:710696"
/country="Russia"
/lat_lon="55.753 N 37.604 E"
/collection_date="1967"
/collected_by="L.V. Kalakoutskii"
protein /locus_tag="Intca_0889"
/note="COGs: COG2366 Protein related to penicillin
acylase; InterPro IPR002692: IPR014395: IPR002034; KEGG:
cfl:Cfla_0835 peptidase S45 penicillin amidase; PFAM:
peptidase S45 penicillin amidase; SPTR: Penicillin
amidase; PFAM: Penicillin amidase"
/transl_table=11
BEGIN
1 MPRAHLARRI ALSVVVLVIV ALVALTILAV STVRRPFPET TGELEVEGLT GRVTVSRDAR
61 GVPTITADSA NDLFRAQGYV SAQDRFFEMD LRRHITAGRL SELVGEGGLD TDRVIRTMGW
121 RRVAEQELPR LAPETRQYLQ AYADGVNAYL KQADSPADLA LEYQVLDWSV PGYRVEPWTP
181 VDSLAWLKAM AWDLKADYED ELARARLSRG GRTSQKQIEL LYPPYPFDRN QPILSEQDWR
241 PGAAASDQQA AAATPMALLK GGQGAFDATA AAMAAIPALI GEGDGVGSNS WVVSGDRTTT
301 GKPLLANDPH LSLGIPSIWT QVNLQCRTVS AQCPFQVSGF SFSGLPGVVI GHNAKVAWGF
361 TNLGADVTDF YLEQVSGDTY LRDGNQEPLE TRRETIKVAG GSDVEILVRS TVHGPILSDA
421 VPAIAESGDQ TAVRGVAQSN RYAVSLAWTA LTPTRTADAI FALNTATGWD DFRSAAALFA
481 VPAQNLVYAD TDGHIGYQTP GLFPVRRSAT PGAVPGSWPS PGWDSQWDWR GFVDFEELPY
541 VLDPQEGFIV TANQAVTASQ KPFLTGVWDY GFRAERIRTL LAGSAKVSPE RMVQIQGDTR
601 NAFAPVLVEH LLGVEVDDFT AQAQLLLRDW DGNQPADKTR DSASAAYYNA VWKHLLEYTF
661 DELPPDMAAS GGTRWMIVLE QLLKDPKNAW WDDKTTPGVT EGSGEILKRA LVDARLDLAR
721 KLGKVPATWR WGRLHQLNLE HRVLGTESVP GVIRDVFNRG GIELGGGNGI VNATSWDASS
781 SGYEVTAGPS MRMVVDLSDL DRSLWVNATG QSGHAYNSHY ADQIDAWAAN EPFPWPFTAR
841 AVAAAQDSEL TLVPPGQPTG
//