LOCUS ADU46720.1 791 aa PRT BCT 08-JAN-2015
DEFINITION Intrasporangium calvum DSM 43043 phosphoenolpyruvate synthase
protein.
ACCESSION CP002343-158
PROTEIN_ID ADU46720.1
SOURCE Intrasporangium calvum DSM 43043
ORGANISM Intrasporangium calvum DSM 43043
Bacteria; Actinobacteria; Micrococcales; Intrasporangiaceae;
Intrasporangium.
REFERENCE 1 (bases 1 to 4024382)
AUTHORS Del Rio,T.G., Chertkov,O., Yasawong,M., Lucas,S., Deshpande,S.,
Cheng,J.F., Detter,C., Tapia,R., Han,C., Goodwin,L., Pitluck,S.,
Liolios,K., Ivanova,N., Mavromatis,K., Pati,A., Chen,A.,
Palaniappan,K., Land,M., Hauser,L., Chang,Y.J., Jeffries,C.D.,
Rohde,M., Pukall,R., Sikorski,J., Goker,M., Woyke,T., Bristow,J.,
Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C., Klenk,H.P.
and Lapidus,A.
TITLE Complete genome sequence of Intrasporangium calvum type strain (7
KIP)
JOURNAL Stand Genomic Sci 3 (3), 294-303 (2010)
PUBMED 21304734
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 4024382)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Bruce,D.,
Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N.,
Chertkov,O., Brettin,T., Tapia,R., Detter,J.C., Han,C., Land,M.,
Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T.,
Wu,D., Pukall,R., Gehrich-Schroeter,G., Schneider,S., Klenk,H.-P.
and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE The complete genome of Intrasporangium calvum DSM 43043
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 4024382)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Bruce,D.,
Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N.,
Chertkov,O., Brettin,T., Detter,J.C., Tapia,R., Han,C., Land,M.,
Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T.,
Wu,D., Pukall,R., Gehrich-Schroeter,G., Schneider,S., Klenk,H.-P.
and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE Direct Submission
JOURNAL Submitted (17-NOV-2010) US DOE Joint Genome Institute, 2800
Mitchell Drive, Walnut Creek, CA 94598-1698, USA
COMMENT URL -- http://www.jgi.doe.gov
JGI Project ID: 4083316
Source DNA and organism available from Hans-Peter Klenk at the
German Collection of Microorganisms and Cell Cultures (DSMZ)
(hans-peter.klenk@dsmz.de)
Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu)
David Bruce (microbe@cuba.jgi-psf.org)
Whole genome sequencing and draft assembly at JGI-PGF
Finishing done by JGI-LANL
Annotation by JGI-ORNL and JGI-PGF
The JGI and collaborators endorse the principles for the
distribution and use of large scale sequencing data adopted by the
larger genome sequencing community and urge users of this data to
follow them. It is our intention to publish the work of this
project in a timely fashion and we welcome collaborative
interaction on the project and analysis.
(http://www.genome.gov/page.cfm?pageID=10506376).
##MIGS-Data-START##
investigation_type :: bacteria_archaea
project_name :: Intrasporangium calvum 7KIP, DSM 43043
collection_date :: 1967
lat_lon :: 55.753 37.604
depth :: Missing
alt_elev :: Missing
country :: Russia
environment :: Air
num_replicons :: Missing
ref_biomaterial :: DSM 43043, ATCC 23552, CUB 430, IFO 12989,
IMET 7816, JCM 3097, KCC A-0097, NBRC 12989,
NCIB 10167
biotic_relationship :: Free living
trophic_level :: Chemoorganotroph
rel_to_oxygen :: Aerobe
isol_growth_condt :: Missing
sequencing_meth :: WGS
assembly :: Newbler v. 2.3 (pre-release)
finishing_strategy :: Finished
GOLD Stamp ID :: Gi02320
Type Strain :: Yes
Greengenes ID :: 90541
Funding Program :: DOE-GEBA 2007
Number of Reads :: 847906 pyrosequencing, 11758818 Illumina
Gene Calling Method :: Prodigal 1.4, GenePRIMP
Isolation Site :: Air in a school dining room
Isolation Comments :: isolated by L.V. Kalakoutskii
Motility :: Nonmotile
Sporulation :: Nonsporulating
Temperature Range :: Mesophile
Temperature Optimum :: 37C
Gram Staining :: Gram+
Diseases :: None
##MIGS-Data-END##
##Genome-Assembly-Data-START##
Finishing Goal :: Finished
Current Finishing Status :: Finished
Assembly Method :: Newbler v. 2.3
Genome Coverage :: 30x
Sequencing Technology :: 454-FLX, 454-Titanium, Illumina GAii
##Genome-Assembly-Data-END##
FEATURES Qualifiers
source /organism="Intrasporangium calvum DSM 43043"
/mol_type="genomic DNA"
/strain="DSM 43043"
/isolation_source="air in a school dining room"
/culture_collection="DSM:43043"
/type_material="type strain of Intrasporangium calvum"
/db_xref="taxon:710696"
/country="Russia"
/lat_lon="55.753 N 37.604 E"
/collection_date="1967"
/collected_by="L.V. Kalakoutskii"
protein /locus_tag="Intca_0159"
/EC_number="2.7.9.2"
/note="COGs: COG0574 Phosphoenolpyruvate synthase/pyruvate
phosphate dikinase; InterProIPR002192: IPR008279:
IPR000121: IPR006319: IPR 018274; KEGG: gob:Gobs_2869
phosphoenolpyruvate synthase; PFAM: pyruvate phosphate
dikinase PEP/pyruvate-binding; PEP-utilising protein
mobile region; PEP-utilizing protein; PRIAM: Pyruvate,
water dikinase; SPTR: Phosphoenolpyruvate synthase;
TIGRFAM: phosphoenolpyruvate synthase; PFAM: PEP-utilising
enzyme, TIM barrel domain; PEP-utilising enzyme, mobile
domain; Pyruvate phosphate dikinase, PEP/pyruvate binding
domain; TIGRFAM: phosphoenolpyruvate synthase"
/transl_table=11
BEGIN
1 MTANILWFRE LGLTDVESVG GKNASLGEMV QHLSKAGVSV PDGFATTAEA YRRFLAHEGL
61 ADRINALLDD LDVDDVRRLA EVGRQIRDAV ESQPFPADLE GEIRTAYDEL VAAAGAEGVS
121 WAVRSSATAE DLPDASFAGQ QETFLNITGI DNILHAIKLV FASLYNDRAI AYRVHSNFDH
181 SGVALSAGVQ RMVRSDIGAS GVMFTIDTES GFPDAVFITS AYGLGEGVVQ GAVNPDEYYV
241 YKPALRAGRP AILKRGIGAK ATKMVYTEDA SVGRTTEFVP VDPADQVRFS LTDDEVTELA
301 QHAVRIEEHY GRPMDIEWAR DGIDGRIYVL QARPETVKSR SGSSTLRRFR MNERGPVVAE
361 GRAIGQKVGS GAVRVLSDVA SMHEFQPGEV LVADMTDPDW EPIMKRASAI VTNRGGRTCH
421 AAIIARELGI PAVVGSGNAT RVLEDGHEVT VSCAEGDTGF VYEGLLDFSV DETALDEMPE
481 VPVKIMMNVG TPEQAFEFSR LPHHGIGLAR LEFIINRQIG IHPRALLELD DQEPALRDEI
541 RGLIGAYDSP REFFVKRLSE GVAMLAAAFA PEPVIVRMSD FKSNEYANLV GGERYEPHEE
601 NPMIGYRGAS RYLSADFAEC FAMEAEALKF VRDEMGLTNV KIMIPFVRTV AEAKGVIDLL
661 AQHGLRRGEN DLQVIMMCEV PSNAVNADAF LEHFDGFSIG SNDMTQLTLG LDRDSALVAG
721 SFDERDPAVK KMLSMAIQAC RAQGKYVGIC GQGPSDHVDL AEWLLDEGIE TMSLNPDTVV
781 DTWLRLAKHA G
//