LOCUS ACU40787.1 603 aa PRT BCT 24-DEC-2013 DEFINITION Actinosynnema mirum DSM 43827 Phosphoenolpyruvate carboxykinase (GTP) protein. ACCESSION CP001630-6813 PROTEIN_ID ACU40787.1 SOURCE Actinosynnema mirum DSM 43827 ORGANISM Actinosynnema mirum DSM 43827 Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae; Actinosynnema. REFERENCE 1 (bases 1 to 8248144) AUTHORS Land,M., Lapidus,A., Mayilraj,S., Chen,F., Copeland,A., Del Rio,T.G., Nolan,M., Lucas,S., Tice,H., Cheng,J.F., Chertkov,O., Bruce,D., Goodwin,L., Pitluck,S., Rohde,M., Goker,M., Pati,A., Ivanova,N., Mavromatis,K., Chen,A., Palaniappan,K., Hauser,L., Chang,Y.J., Jeffries,C.C., Brettin,T., Detter,J.C., Han,C., Chain,P., Tindall,B.J., Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C. and Klenk,H.P. TITLE Complete genome sequence of Actinosynnema mirum type strain (101) JOURNAL Stand Genomic Sci 1 (1), 46-53 (2009) PUBMED 21304636 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 8248144) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE The complete genome of Actinosynnema mirum DSM 43827 JOURNAL Unpublished REFERENCE 3 (bases 1 to 8248144) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE Direct Submission JOURNAL Submitted (29-MAY-2009) US DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4082379 Source DNA and organism available from Hans-Peter Klenk at the German Collection of Microorganisms and Cell Cultures (DSMZ) (hans-peter.klenk@dsmz.de) Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu) David Bruce (microbe@cuba.jgi-psf.org) Whole genome sequencing and draft assembly at JGI-PGF Finishing done by JGI-LANL Annotation by JGI-ORNL and JGI-PGF The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##Metadata-START## Organism Display Name :: Actinosynnema mirum 101, DSM 43827 Culture Collection ID :: DSM 43827, ATCC 29888, NBRC 14064 GOLD Stamp ID :: Gc01024 Funding Program :: DOE-GEBA 2007 Assembly Method :: Newbler version 1.1.02.15, Phrap Sequencing Depth :: 8.9x Sanger; 20x pyrosequence Gene Calling Method :: Prodigal Isolation Site :: "Blade of grass from Raritan River NJ, USA" Collection Date :: September 1976 Isolation Country :: USA Latitude :: 40.491816 Longitude :: -74.322087 Oxygen Requirement :: Aerobe Motility :: Nonmotile Sporulation :: Sporulating Temperature Range :: Mesophile Temperature Optimum :: 10-30C Gram Staining :: gram+ Biotic Relationship :: Free living Diseases :: None Habitat :: Soil Phenotypes :: Nocardicin A producer Energy Source :: Chemoorganotroph ##Metadata-END## FEATURES Qualifiers source /organism="Actinosynnema mirum DSM 43827" /mol_type="genomic DNA" /strain="DSM 43827" /type_material="type strain of Actinosynnema mirum" /db_xref="taxon:446462" protein /locus_tag="Amir_6994" /EC_number="4.1.1.32" /inference="protein motif:PRIAM:4.1.1.32" /note="PFAM: phosphoenolpyruvate carboxykinase (GTP); KEGG: pzu:PHZ_c2232 phosphoenolpyruvate carboxykinase (GTP)" /transl_table=11 /db_xref="InterPro:IPR008209" BEGIN 1 MTAVTIPGLD QAPTDHQELL AWVREIAELT SPDRVVWADG TQREWAELTG KLVDAGTFVP 61 LEKKPNSFWA ASDPTDVARV EERTFICSVD PADSGPTNNW VEPAEMKATM TELYRGSMRG 121 RTMYVIPFCM GPLTAEKPML GVEITDSEYV VVSMHIMTRM GTRALELFGD GVPYVKALHS 181 LGAPLAPGQD DVAWPCNDTK YITHFPEERT IWSFGSGYGG NALLGKKCYS LRIASVMARD 241 EGWLAEHMLI LKLTSPESKV YYIAAAFPSA CGKTNLAMLQ PTIPGWKVET LGDDIAWMRF 301 GEDGRLYAVN PENGFFGVAP GTDYHTNPNA MRTIEKGNSL FTNVALTDDG DVWWEGMGEP 361 PAHLTSWKKA DWTPGSDEPS SHPNSRYCTP IEQCDIKAPE WDDPNGVPIS AIFFGGRRAT 421 TVPLITESRD WQHGVFMGAT LSSETTAAAV GQVGVVRRDP MAMLPFIGYH AGDYFQHWID 481 TGKGADADKL PKVFYVNWFR RGEDKRFLWP GFGENSRVLK WAVERIEGRA AARETPIGHV 541 PTAEALDLSE LGAPVEDVEA SLAFDADEWR AELPLIEEWF AKIGDKVPTA LRDELEALKQ 601 RLG //