LOCUS ACU37815.1 524 aa PRT BCT 24-DEC-2013 DEFINITION Actinosynnema mirum DSM 43827 drug resistance transporter, EmrB/QacA subfamily protein. ACCESSION CP001630-3841 PROTEIN_ID ACU37815.1 SOURCE Actinosynnema mirum DSM 43827 ORGANISM Actinosynnema mirum DSM 43827 Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae; Actinosynnema. REFERENCE 1 (bases 1 to 8248144) AUTHORS Land,M., Lapidus,A., Mayilraj,S., Chen,F., Copeland,A., Del Rio,T.G., Nolan,M., Lucas,S., Tice,H., Cheng,J.F., Chertkov,O., Bruce,D., Goodwin,L., Pitluck,S., Rohde,M., Goker,M., Pati,A., Ivanova,N., Mavromatis,K., Chen,A., Palaniappan,K., Hauser,L., Chang,Y.J., Jeffries,C.C., Brettin,T., Detter,J.C., Han,C., Chain,P., Tindall,B.J., Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C. and Klenk,H.P. TITLE Complete genome sequence of Actinosynnema mirum type strain (101) JOURNAL Stand Genomic Sci 1 (1), 46-53 (2009) PUBMED 21304636 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 8248144) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE The complete genome of Actinosynnema mirum DSM 43827 JOURNAL Unpublished REFERENCE 3 (bases 1 to 8248144) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE Direct Submission JOURNAL Submitted (29-MAY-2009) US DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4082379 Source DNA and organism available from Hans-Peter Klenk at the German Collection of Microorganisms and Cell Cultures (DSMZ) (hans-peter.klenk@dsmz.de) Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu) David Bruce (microbe@cuba.jgi-psf.org) Whole genome sequencing and draft assembly at JGI-PGF Finishing done by JGI-LANL Annotation by JGI-ORNL and JGI-PGF The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##Metadata-START## Organism Display Name :: Actinosynnema mirum 101, DSM 43827 Culture Collection ID :: DSM 43827, ATCC 29888, NBRC 14064 GOLD Stamp ID :: Gc01024 Funding Program :: DOE-GEBA 2007 Assembly Method :: Newbler version 1.1.02.15, Phrap Sequencing Depth :: 8.9x Sanger; 20x pyrosequence Gene Calling Method :: Prodigal Isolation Site :: "Blade of grass from Raritan River NJ, USA" Collection Date :: September 1976 Isolation Country :: USA Latitude :: 40.491816 Longitude :: -74.322087 Oxygen Requirement :: Aerobe Motility :: Nonmotile Sporulation :: Sporulating Temperature Range :: Mesophile Temperature Optimum :: 10-30C Gram Staining :: gram+ Biotic Relationship :: Free living Diseases :: None Habitat :: Soil Phenotypes :: Nocardicin A producer Energy Source :: Chemoorganotroph ##Metadata-END## FEATURES Qualifiers source /organism="Actinosynnema mirum DSM 43827" /mol_type="genomic DNA" /strain="DSM 43827" /type_material="type strain of Actinosynnema mirum" /db_xref="taxon:446462" protein /locus_tag="Amir_3948" /inference="protein motif:TFAM:TIGR00711" /note="TIGRFAM: drug resistance transporter, EmrB/QacA subfamily; PFAM: major facilitator superfamily MFS_1; KEGG: bph:Bphy_3357 EmrB/QacA family drug resistance transporter" /transl_table=11 /db_xref="InterPro:IPR001411" /db_xref="InterPro:IPR001958" /db_xref="InterPro:IPR004638" /db_xref="InterPro:IPR006311" /db_xref="InterPro:IPR007114" /db_xref="InterPro:IPR011701" BEGIN 1 MTDTAVPATG APSTSRRDVI QAMTGLMLGM FVSILASTVV SNALPRIISD LDGSQSVYTW 61 VVTAELLAMT ATVPLWGKLA DLYSRKLLIQ LSLGLFVVGS LLAGFSPNVE VLIVSRVVQG 121 LGAGGMTALS TIVMAAMIPP RELGRYAGLF GAVFGIGTVA GPLIGGFLVD TSWLGWRWCF 181 FIGVPFTLAA IVLLQRTLHL PLVRKDSVRI DYLGAALITG GVSTLLIWST LAGGSFEWVS 241 AWSAVLVGGG VLLLALAVWV ESRAADPVVP LRLFANRTVS LTTIASVLVG VAMFGGTVFL 301 SQYFQIGLGK TPTQAGLMSL PMVFGLFLSS TIAGQLITKW GRWKVFLVAG AAIMVVGMLL 361 LSTIDANTTV LVVGVHMAVL GVGVGMVMQN LVLAAQNDVP AKDLGATTST LTFFRSLGGS 421 IGVSALGAVL ANRVSTLMTE KLTALGVPAD MLASGGGHTA VPDLSALPEP IRLVVRDVYA 481 TATAELFLIG APIAAIALLA VLFIKEKPLK EQSGDERLAA ELPH //