LOCUS ACU37766.1 2827 aa PRT BCT 24-DEC-2013
DEFINITION Actinosynnema mirum DSM 43827 amino acid adenylation domain
protein protein.
ACCESSION CP001630-3792
PROTEIN_ID ACU37766.1
SOURCE Actinosynnema mirum DSM 43827
ORGANISM Actinosynnema mirum DSM 43827
Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
Actinosynnema.
REFERENCE 1 (bases 1 to 8248144)
AUTHORS Land,M., Lapidus,A., Mayilraj,S., Chen,F., Copeland,A., Del
Rio,T.G., Nolan,M., Lucas,S., Tice,H., Cheng,J.F., Chertkov,O.,
Bruce,D., Goodwin,L., Pitluck,S., Rohde,M., Goker,M., Pati,A.,
Ivanova,N., Mavromatis,K., Chen,A., Palaniappan,K., Hauser,L.,
Chang,Y.J., Jeffries,C.C., Brettin,T., Detter,J.C., Han,C.,
Chain,P., Tindall,B.J., Bristow,J., Eisen,J.A., Markowitz,V.,
Hugenholtz,P., Kyrpides,N.C. and Klenk,H.P.
TITLE Complete genome sequence of Actinosynnema mirum type strain (101)
JOURNAL Stand Genomic Sci 1 (1), 46-53 (2009)
PUBMED 21304636
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 8248144)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N.,
Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C.,
Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F.,
Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE The complete genome of Actinosynnema mirum DSM 43827
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 8248144)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N.,
Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C.,
Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F.,
Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE Direct Submission
JOURNAL Submitted (29-MAY-2009) US DOE Joint Genome Institute, 2800
Mitchell Drive, Walnut Creek, CA 94598-1698, USA
COMMENT URL -- http://www.jgi.doe.gov
JGI Project ID: 4082379
Source DNA and organism available from Hans-Peter Klenk at the
German Collection of Microorganisms and Cell Cultures (DSMZ)
(hans-peter.klenk@dsmz.de)
Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu)
David Bruce (microbe@cuba.jgi-psf.org)
Whole genome sequencing and draft assembly at JGI-PGF
Finishing done by JGI-LANL
Annotation by JGI-ORNL and JGI-PGF
The JGI and collaborators endorse the principles for the
distribution and use of large scale sequencing data adopted by the
larger genome sequencing community and urge users of this data to
follow them. It is our intention to publish the work of this
project in a timely fashion and we welcome collaborative
interaction on the project and analysis.
(http://www.genome.gov/page.cfm?pageID=10506376).
##Metadata-START##
Organism Display Name :: Actinosynnema mirum 101, DSM 43827
Culture Collection ID :: DSM 43827, ATCC 29888, NBRC 14064
GOLD Stamp ID :: Gc01024
Funding Program :: DOE-GEBA 2007
Assembly Method :: Newbler version 1.1.02.15, Phrap
Sequencing Depth :: 8.9x Sanger; 20x pyrosequence
Gene Calling Method :: Prodigal
Isolation Site :: "Blade of grass from Raritan River NJ,
USA"
Collection Date :: September 1976
Isolation Country :: USA
Latitude :: 40.491816
Longitude :: -74.322087
Oxygen Requirement :: Aerobe
Motility :: Nonmotile
Sporulation :: Sporulating
Temperature Range :: Mesophile
Temperature Optimum :: 10-30C
Gram Staining :: gram+
Biotic Relationship :: Free living
Diseases :: None
Habitat :: Soil
Phenotypes :: Nocardicin A producer
Energy Source :: Chemoorganotroph
##Metadata-END##
FEATURES Qualifiers
source /organism="Actinosynnema mirum DSM 43827"
/mol_type="genomic DNA"
/strain="DSM 43827"
/type_material="type strain of Actinosynnema mirum"
/db_xref="taxon:446462"
protein /locus_tag="Amir_3892"
/inference="protein motif:TFAM:TIGR01733"
/note="TIGRFAM: amino acid adenylation domain protein;
PFAM: AMP-dependent synthetase and ligase; Beta- ketoacyl
synthase; short-chain dehydrogenase/reductase SDR;
condensation domain protein; KR domain protein;
Thioesterase; phosphopantetheine-binding; KEGG:
pen:PSEEN2718 polyketide synthase (terminal component)"
/transl_table=11
/db_xref="InterPro:IPR000873"
/db_xref="InterPro:IPR001031"
/db_xref="InterPro:IPR001242"
/db_xref="InterPro:IPR002198"
/db_xref="InterPro:IPR006162"
/db_xref="InterPro:IPR006163"
/db_xref="InterPro:IPR010071"
/db_xref="InterPro:IPR013968"
/db_xref="InterPro:IPR014030"
/db_xref="InterPro:IPR014031"
BEGIN
1 MTSIARRVHD RALRRPDALA VSDGHRHLTY AELDAGAAAV AETLSRQGVR PGDAVAVGLP
61 RSLELVQAML GVLRLGGQVV PLDRQSPPAR RDVILADAGV VAVIEDELPG LTPVEAGTPA
121 EPAPASFLFY TSGTTGRPKG VEVRDAGVLR LAEPGWLDHG TRFACLANPA FDAISFEVWA
181 PLLTGGTCVI LDDETVRDPR AFADALVRER VDTMFVTATL FNAVAETVPG SFATVRRVVI
241 GGEQLNAPLI RRWYRDNPDS PTLLQNGYGP TETTTFALSH PIPRDLDAEV VPIGRPLPGT
301 GAVAVTPDGR VAAEGEVAEL HLSGAGVALG YRGLPEETGR KFVRLPWLDG GRARHYRTGD
361 LVRVLPGGVF AYVGRTDRQV KVRGFRIEPG ELEQRLLGHP AIRQVHVAVR RAATAELLAY
421 VVADELSFDD FEAHLAETVP AYMRPHHVFR LAGLPRNANG KVDEAALLAG GFPAWRPDTD
481 GDATAWEQEV LGVVAEILDV PDLRPGDTWL RSGGDSLKAL RLRFAIRERW GVDLAPSVVH
541 RGDLADLAAS ISAAGRAAYP EPGAPTTATT APATSEQQRL WLLQRRNPAS TAYHVPLAFR
601 VAGDPDLAAL RRDVRGLVER HPALRTAFEA APDGLRQVER AAYDPWTEPG GDFFTAPFDL
661 ASGRLLRAAW RPDGTFLLCL HHIAVDGWSL NVLFRELSGA RPRPGARTPF DFADWQARWF
721 ASPGYRAQLA ERADDAEPLG EASLAGRLRT TRVDRERVDR WAAELGLTRF QLLLGVFARS
781 LCAVTGRTRP RIATPVANRP VREFEDGVGM FANTVRLPLS VDPEEDLAAQ LRRLGDAAGA
841 ALDHQDVAFA DLDPAYAAFD YLFVLENTDF EALRLPGCSV SPLWIEAAEA KCALTLSVVE
901 RPDGFDCLWE YGEGHFTEAE ATALGEVFDR ALRGDARMPD HHDHGRGAEA DLVWETVAEG
961 FARQVALTPD APALTGGTTL TYAELDAHAA RLAAALPDDA TDVALYFRPS AEHVVALLAL
1021 ARLNITAVPL DPAYPPALLR EMLDQARPSC VLLAPGEEFD TDVERRTVTL TDGPVPDVPA
1081 RHAGRPLYTL FTSGSTGAPK GVRVPDRTLA NLVQWQDLPP AATQQFSMLS FDVSFQEVFT
1141 TLCGGGRLHL VRPEWRHDVP ALLEQLETAG IERIFLPYVA LQLLAEHGAR LGLHPSRLRD
1201 VITAGEQLVC TPAIRRWFAG LPGARLHNHY GPTETHVVSA LVLDGDPARW PERPAIGRPV
1261 ANAVLRVADA SGAPLPPGQV GELLIGGLAA NRCYLGDVAP EKFAELPGLH YRSGDLAHFD
1321 RAGLLHFDGR ADRQVKFSGH RLELGQVEAA LLRHPDVVNA VVLPQGDRLA AFLECRRTPS
1381 ARELADFLEP LLPPHARVDR FRVVEALPRT PSGKLDRDAA PGVRGTDLHR PSVTPSVTPS
1441 VTPSVTPSSE LAALFEEVTG APIGPDETFF AAGATSLSLM RFHLRCAEAG YRFTAADLFE
1501 HVTLRDLTRH LGTGPAEART SAGAAADEPV AVIGMAVRLP GAPDLASFWD LVVSGRRGVE
1561 EFPAAEGLVG ARSLLDDPLA FDPEHFGVSH RDAALMDPQQ RQLLMTCVEA LAHAGIARPD
1621 RVGIVAGCGE STYFQEMLRE ADPAALPDGF HLALHHEKDF LATKVAYHLD LTGPALTTQT
1681 ACSSSLVAVH LAAGMLRGGE ADVMLAGGVL IDRTLDGGYR YRPQHIFSPD GHCRPFSDDA
1741 DGTVGASGVG VVVLKPLSAA RRAGDTVYSV LTGSALTNDG AVKQGYSAPS PAGQRAAIRA
1801 ALRRAGRSGA DVGYVEAHGT GTRLGDPIEV SALREAMPEA SACALSSVKS QLGHLGAAAG
1861 VVGLIRATLA VHHGVLPPTA DFRAPNPALD LGPFRVPTRA EPWPAGPRVA GVSSFGIGGT
1921 NAHVVVESAP TPPRERREPV RCLVLSARSA EALRLDAARV ADHLSAHPES FVDVLRHLQS
1981 GRPHGRWRLA AAVEDAAEAA VWLRAAQPQE TPAPPLSRDP RPSPGSGASP AEVARVWLGG
2041 GAVDWGRSTA PAPWDFPPPA FVTRDFDFPR RAAVARLPEA EWLRQPTWIR LRRTAGATRT
2101 DRVLVISGEG PLTAPALDAA YPRVVRVHAA ERFARLGPDA FEADLADTAC LRRVLDSLPA
2161 QEIDWLHTPP LGITGPLGEA SVRHARWACV DTPAALLRAA HDRPLRTWWV SAGAAPATTG
2221 VTRPEAGLLA GVGAVGPQES GIPGHWVDLP GPDLAPLADL LTGPTPPARV ALRAGFWWAE
2281 DDAPVRAGEP LAVDGVHLVL GGTGGIGAAV AEELLTRGAG RVVLLARDPR LPDALTPWAD
2341 RVDLLAADLT TVTADAIPGP LAGIVHAAGN PDGALIATRG EGTQAVKLAG ALLVEQLVER
2401 DRPAQVVYCS SLSARFGGVG QLDYAAANGL LDALAHRDGP TARLSIAWDV WRDTGMAVTA
2461 LRTDARHQAH LAVGMSAEEG RRTFARALDA CLPHVLVATT GLDNARAFHE RETAPVTASP
2521 AGDAGAELTE EVRALLGVAE LRPDVPLHDL GADSLTLLEV VDAVKRLFGV DLDLAWLGPE
2581 VTMAGLLAKL AEAGDTGDVA VQVWREGAGR EVVCLVHPVG GDVHAYRPLV EALDPALGVC
2641 VIADPALGLP EPPGWSVEDR ARRYYAALRA TLPGRTWQLA GWSFGAWVAT GMAAEAEADG
2701 QPVRALHLID PPAPGASAAD YSDAQLEAVF ERELGASSGT AGEHAQRLAR ACRANLRSMG
2761 GHRLPLLTAT PSHVWLAVEP EPGSPVADRG GWRALLPEPS HWHQLPATHY GVVRAPHVTA
2821 VAEAIGL
//