LOCUS ACU37468.1 2098 aa PRT BCT 24-DEC-2013
DEFINITION Actinosynnema mirum DSM 43827 amino acid adenylation domain
protein protein.
ACCESSION CP001630-3494
PROTEIN_ID ACU37468.1
SOURCE Actinosynnema mirum DSM 43827
ORGANISM Actinosynnema mirum DSM 43827
Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
Actinosynnema.
REFERENCE 1 (bases 1 to 8248144)
AUTHORS Land,M., Lapidus,A., Mayilraj,S., Chen,F., Copeland,A., Del
Rio,T.G., Nolan,M., Lucas,S., Tice,H., Cheng,J.F., Chertkov,O.,
Bruce,D., Goodwin,L., Pitluck,S., Rohde,M., Goker,M., Pati,A.,
Ivanova,N., Mavromatis,K., Chen,A., Palaniappan,K., Hauser,L.,
Chang,Y.J., Jeffries,C.C., Brettin,T., Detter,J.C., Han,C.,
Chain,P., Tindall,B.J., Bristow,J., Eisen,J.A., Markowitz,V.,
Hugenholtz,P., Kyrpides,N.C. and Klenk,H.P.
TITLE Complete genome sequence of Actinosynnema mirum type strain (101)
JOURNAL Stand Genomic Sci 1 (1), 46-53 (2009)
PUBMED 21304636
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 8248144)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N.,
Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C.,
Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F.,
Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE The complete genome of Actinosynnema mirum DSM 43827
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 8248144)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N.,
Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C.,
Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F.,
Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE Direct Submission
JOURNAL Submitted (29-MAY-2009) US DOE Joint Genome Institute, 2800
Mitchell Drive, Walnut Creek, CA 94598-1698, USA
COMMENT URL -- http://www.jgi.doe.gov
JGI Project ID: 4082379
Source DNA and organism available from Hans-Peter Klenk at the
German Collection of Microorganisms and Cell Cultures (DSMZ)
(hans-peter.klenk@dsmz.de)
Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu)
David Bruce (microbe@cuba.jgi-psf.org)
Whole genome sequencing and draft assembly at JGI-PGF
Finishing done by JGI-LANL
Annotation by JGI-ORNL and JGI-PGF
The JGI and collaborators endorse the principles for the
distribution and use of large scale sequencing data adopted by the
larger genome sequencing community and urge users of this data to
follow them. It is our intention to publish the work of this
project in a timely fashion and we welcome collaborative
interaction on the project and analysis.
(http://www.genome.gov/page.cfm?pageID=10506376).
##Metadata-START##
Organism Display Name :: Actinosynnema mirum 101, DSM 43827
Culture Collection ID :: DSM 43827, ATCC 29888, NBRC 14064
GOLD Stamp ID :: Gc01024
Funding Program :: DOE-GEBA 2007
Assembly Method :: Newbler version 1.1.02.15, Phrap
Sequencing Depth :: 8.9x Sanger; 20x pyrosequence
Gene Calling Method :: Prodigal
Isolation Site :: "Blade of grass from Raritan River NJ,
USA"
Collection Date :: September 1976
Isolation Country :: USA
Latitude :: 40.491816
Longitude :: -74.322087
Oxygen Requirement :: Aerobe
Motility :: Nonmotile
Sporulation :: Sporulating
Temperature Range :: Mesophile
Temperature Optimum :: 10-30C
Gram Staining :: gram+
Biotic Relationship :: Free living
Diseases :: None
Habitat :: Soil
Phenotypes :: Nocardicin A producer
Energy Source :: Chemoorganotroph
##Metadata-END##
FEATURES Qualifiers
source /organism="Actinosynnema mirum DSM 43827"
/mol_type="genomic DNA"
/strain="DSM 43827"
/type_material="type strain of Actinosynnema mirum"
/db_xref="taxon:446462"
protein /locus_tag="Amir_3578"
/inference="protein motif:TFAM:TIGR01733"
/note="TIGRFAM: amino acid adenylation domain protein;
PFAM: AMP-dependent synthetase and ligase;
phosphopantetheine-binding; condensation domain protein;
KEGG: mxa:MXAN_3636 non-ribosomal peptide synthetase"
/transl_table=11
/db_xref="InterPro:IPR000873"
/db_xref="InterPro:IPR001242"
/db_xref="InterPro:IPR006162"
/db_xref="InterPro:IPR006163"
/db_xref="InterPro:IPR010071"
BEGIN
1 MNEGAPHTAN GPAAEVSSAQ RRLWFLEQLA RGGSDNLLPL ALRVRGALDV PALERALAGV
61 VTRHEVLRTR FTAVDGEPVP VLDPPDAVVL RHLDAASPQE VFERELRRPL DLAVEPPVRA
121 VLARLGAEDH LLLLVVHHIA VDGWSWDVLL RELAAGYRGE HVPAPAHQYA DFAAWQNARR
181 TPERLARLLA HWTGRLTGVT PLALPTDRPR PEVWDGSGDV VRFVVPPDLL ARVDAAARER
241 RATRYQVLLA VYQSLLGHHS GRTDVATCTT MTDRGGPLDT ANLIGPFVNT VVLRTDLGGR
301 PSFDALLRRV RDGVLKDLSH AEAPFDRVVG ALGLERDLSR HPLAQASFTL LNTAHQGASL
361 QGLDAELVRT PLDGTDMEVF LDLTLMPDGS LLARLQYATA LFDRATAEQL ADGFLALLTA
421 VLDDPDAPVA DLAALLPPLP GRVLLDTWGR PAAVQVPEPL RVRGSGERAA LVCGADTVTY
481 DRLEALTGGL AALLREAGVR RGDAVGVCLP RGTWSVVAML AIWRAGGVHV PLDAALPVER
541 RAFMVAEAGV RHAVGDTGVA GVRHLDVADV DPDAGVDPVD PDPAELAYVI FTSGSTGLPK
601 AVGVEHGALA AHVTAARDRY AVTEADRVLA FSSFSFDASL DQLLPALTRG ATVVLRGDEP
661 WLPASVPALV AAHGLTVLNL PPTYWSELAL SLASGPVDSS TDLAGLRLLI LGGEEVPTGA
721 LAAWRGHAPH VRVLNAYGPT EAVVTATTHE VAGAQSGGAQ SGAAQSGGAQ SGRVPIGRPL
781 GGRRVLVVDA GGDPVGVGEP GELLVGGAEL ARGYLGRPAL TAERFVPDPA GGRLYRTGDL
841 VRWRADGELE FLGRADDQVK IRGYRVELGE VESALSGCAG VLAAAAAVKA DAHGGQSLVG
901 YVVPAGAPTA ATPTAAELRA ELARALPHYA VPAEIVVLDA LPVTTSGKLD RAALPDPDRS
961 AAPAGDHAEP RDDVEREIAR IWAEVLGVPR VGLDDGFFDL GGHSLLATMA VSRVAERLGR
1021 DVELRVLFEN PRIREFGPLV AAARPVATAR VVPVDRSGPL PMSFAQERLW FLDRVSDSGE
1081 DYVLWFSWRV GGPLDADAWE AALGDVVARH EVLRTALIEV DGHPVQLVRH DVPVPLERRA
1141 GHDVEQVRRE AKEFAAARFE LDRPPLVRSG LWRLGPDEHV LVLAFHHVAT DGWSKDVVIG
1201 DLARCYTARV AGRTPELPAL PVQYGDYAVW QRDLLDSGAL DGQLAHWSAA LDGVAALDLP
1261 TDHPRPATRS ARGGAVETPL PPELAEGVDA LAHRFGTTRF TVLLAVLQSV LARWSGQTDV
1321 AVGTPVAGRG RVELEGLVGF FVNTVVLRGD LSGDPTFAEL ITRARDTALT AFGNQDVPFE
1381 RLVERLRPER DLSRNPLFQV MFDVQESAVS TGLSLPGLDL TGFALPWESA KFDLTATFLA
1441 HPDRFSLDVE YSADLFDAAT ATRLAEHVVR ALRAAIADPD SVAARADLLS PGEHAALTAP
1501 PPPAPAAPLR LGGVPEATAL VCGERAVTYG ELDGLVGGLA SALVAGGVRR GDPVGVCLSR
1561 GPWSVAAMLA VWRAGGAYVP LDPALPEERL RYALAEAGAR VAVVDERTSG PLTRLGAQVV
1621 PVGSTTPGAH PAADPDPADP DPTDLAYVIF TSGSTGRPKA VGVDHGPLAA HVAAARELYA
1681 LTPDDRVLSF ASLSFDASLE QLLPALAAGA TVVLRPDEVW SVDELAAEVR GRGVTVAELT
1741 PSYWAQVVAR LGDLAPDLAG LRLLVTGGEA LPAEPLGRWF ELLPHVPVVN SYGPTEAVIA
1801 ATAHLVTGPV AGRVPIGTAL GARTLHVVDR HDRPVPDGVP GELLIGGPHL ARGYLGHPEL
1861 TAERFPANPF GPGRAYRTGD RVRRLPGGEL EFLGRVDDQV KIRGHRIEPG EAAAVLRECP
1921 GVRGAAVVVR DVGGEPALVG YATGEGLDPG GLTTWCRDRL PGYLVPTAVV LLDALPLTVQ
1981 GKLDTAALPD PTTTAPGEHV PPGTPTEVVL AQIWAEVLGV DRVGLHDDFF ALGGHSLRAV
2041 ATASRVRTAF DCPLAVRELF DNPTVALLAA EVERLLVEQI SAMSEDEIDL SLSVDAAF
//