LOCUS ACU37199.1 3408 aa PRT BCT 24-DEC-2013
DEFINITION Actinosynnema mirum DSM 43827 Acyl transferase protein.
ACCESSION CP001630-3225
PROTEIN_ID ACU37199.1
SOURCE Actinosynnema mirum DSM 43827
ORGANISM Actinosynnema mirum DSM 43827
Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
Actinosynnema.
REFERENCE 1 (bases 1 to 8248144)
AUTHORS Land,M., Lapidus,A., Mayilraj,S., Chen,F., Copeland,A., Del
Rio,T.G., Nolan,M., Lucas,S., Tice,H., Cheng,J.F., Chertkov,O.,
Bruce,D., Goodwin,L., Pitluck,S., Rohde,M., Goker,M., Pati,A.,
Ivanova,N., Mavromatis,K., Chen,A., Palaniappan,K., Hauser,L.,
Chang,Y.J., Jeffries,C.C., Brettin,T., Detter,J.C., Han,C.,
Chain,P., Tindall,B.J., Bristow,J., Eisen,J.A., Markowitz,V.,
Hugenholtz,P., Kyrpides,N.C. and Klenk,H.P.
TITLE Complete genome sequence of Actinosynnema mirum type strain (101)
JOURNAL Stand Genomic Sci 1 (1), 46-53 (2009)
PUBMED 21304636
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 8248144)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N.,
Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C.,
Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F.,
Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE The complete genome of Actinosynnema mirum DSM 43827
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 8248144)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N.,
Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C.,
Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F.,
Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE Direct Submission
JOURNAL Submitted (29-MAY-2009) US DOE Joint Genome Institute, 2800
Mitchell Drive, Walnut Creek, CA 94598-1698, USA
COMMENT URL -- http://www.jgi.doe.gov
JGI Project ID: 4082379
Source DNA and organism available from Hans-Peter Klenk at the
German Collection of Microorganisms and Cell Cultures (DSMZ)
(hans-peter.klenk@dsmz.de)
Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu)
David Bruce (microbe@cuba.jgi-psf.org)
Whole genome sequencing and draft assembly at JGI-PGF
Finishing done by JGI-LANL
Annotation by JGI-ORNL and JGI-PGF
The JGI and collaborators endorse the principles for the
distribution and use of large scale sequencing data adopted by the
larger genome sequencing community and urge users of this data to
follow them. It is our intention to publish the work of this
project in a timely fashion and we welcome collaborative
interaction on the project and analysis.
(http://www.genome.gov/page.cfm?pageID=10506376).
##Metadata-START##
Organism Display Name :: Actinosynnema mirum 101, DSM 43827
Culture Collection ID :: DSM 43827, ATCC 29888, NBRC 14064
GOLD Stamp ID :: Gc01024
Funding Program :: DOE-GEBA 2007
Assembly Method :: Newbler version 1.1.02.15, Phrap
Sequencing Depth :: 8.9x Sanger; 20x pyrosequence
Gene Calling Method :: Prodigal
Isolation Site :: "Blade of grass from Raritan River NJ,
USA"
Collection Date :: September 1976
Isolation Country :: USA
Latitude :: 40.491816
Longitude :: -74.322087
Oxygen Requirement :: Aerobe
Motility :: Nonmotile
Sporulation :: Sporulating
Temperature Range :: Mesophile
Temperature Optimum :: 10-30C
Gram Staining :: gram+
Biotic Relationship :: Free living
Diseases :: None
Habitat :: Soil
Phenotypes :: Nocardicin A producer
Energy Source :: Chemoorganotroph
##Metadata-END##
FEATURES Qualifiers
source /organism="Actinosynnema mirum DSM 43827"
/mol_type="genomic DNA"
/strain="DSM 43827"
/type_material="type strain of Actinosynnema mirum"
/db_xref="taxon:446462"
protein /locus_tag="Amir_3293"
/inference="protein motif:PFAM:PF00698"
/note="PFAM: Acyl transferase; short-chain
dehydrogenase/reductase SDR; Beta-ketoacyl synthase; KR
domain protein; Erythronolide synthase docking;
phosphopantetheine-binding; NAD-dependent
epimerase/dehydratase; KEGG: mxa:MXAN_4527 polyketide
synthase"
/transl_table=11
/db_xref="InterPro:IPR001509"
/db_xref="InterPro:IPR002198"
/db_xref="InterPro:IPR006162"
/db_xref="InterPro:IPR006163"
/db_xref="InterPro:IPR013968"
/db_xref="InterPro:IPR014030"
/db_xref="InterPro:IPR014031"
/db_xref="InterPro:IPR014043"
/db_xref="InterPro:IPR015083"
BEGIN
1 MANDERLLSY LKRVTADLHR TRERLREAES GADEPIAIVG MACRFPGGVR TPDELWELVA
61 SGRDGIGPFP DDRGWDLGAL FDPDPDATGR SYVTEGGFLD DAALFDAGFF GISPREALAT
121 DPQQRVLLET AWETFEQAGI DPTSLSGQDV GVFTGVANGD YALTVDRVPE GFEGYLGIGG
181 AGSIASGRIS YSLGLEGPAV TLDTGCSSSL VAMHWAGHAL RARECSLALA GGVMVMATPG
241 GFVGFSRQRG LARDGRCKSF GDGADGTSWS EGVGLLLLER LSDARANGHE VLAVVRGSAI
301 NQDGASNGLT APNGPSQQRV IRAALDAAGL GYADVDAVEA HGTATVLGDP IEAQALLNTY
361 GRHRDGAQPL YLGSVKSNLG HTQAAAGVAG VIKAVQAMRH GVLPPTLNVG TPTTKVDWSS
421 GAVEVLAEAR PWPETGRPRR VGVSSFGVSG TNAHVILEQA PEHEPAPEEP GGRGPVAAGG
481 ATPWTLSGRT PAALADQARR LAGHVTADLR AEDVGFSLAT TRAHLEHRAV VVGSDGLAAL
541 AEGLPSALVT TGEADVSGKA VFVFPGQGAQ WIGMGAELID ASPVFAERLR ECAEALEPFV
601 DFDLVEVLRE RGSLERVDVV QPASWAVMVS LAALWRSLGV EPDAVVGHSQ GEIAAAAVSG
661 ALSLPDAAAV VALRSKAIAQ DLAGLGGMMS VALPADDVDL SGYPDRLWIA AHNGPTSTVV
721 AGDVDALREL HAHYEATEVR ARIIPVDYAS HTGHVDTIRE RLAEALSGVT PRAGEVPWLS
781 TVTGQWTIGE DADADYWFRN LRGAVGFHTA ITTLAEQGHR VFVEVSSHPV LTTAIEATLE
841 GTGPTAVTGT LRRDDGGPDR LLTSLATLHV RGVHVDWKAV FAGTGARRVP LPTYAFQHER
901 YWLDRGAAAG DVTGAGLADA AHPLLAAVAQ LPGTGGVLLS GRLSRATHPW LAEHVVNGTA
961 LVPGTALVEL ALRAGDEVDA PVLRELVITR PMPVPERGFL HVQVDVAGAA DDGARAVRIW
1021 SRAEDAASET ARWTEHATGS LAPDDAAPPA RASGAWPPEG AAAVDVDDFY DRLAGAGYEY
1081 GPLFQGLTAA WAGDGQAWAE VVLPGEAGGF GAHPALLDAA LHVGTFCLPG GPGSRTLLPF
1141 AWTGVRLHAT GATAVRVHAR ATGDDGLVVE LRDADGDPVV TVDALVLRDA DPADAQAPDV
1201 TADALWRVRW VEQPPAPAAP GWVLLGGPSG HAGFAALPVF ADPAAVAALP EGDRPAVVVV
1261 DTTAWREPGR DVPGAARAFV VRALELLVAW LREDALAGTR LVLVTSGAVP VRADAEVTDP
1321 AAAAVWGLAR SAQSEHPDRV WLLDVDEPGA APGALASPEP QLAVRAGAGF APRLARAEAA
1381 PGALPVDGPV LVTGGTGTLG ALVARHLVTA HGARNLHLVS RRGPDASGAR ELLDELRGLG
1441 AEVELSACDV ADRVALAALL GRVRPAAVVH AAGAVDDGLL TDLTADRFDA VLRPKVDAVA
1501 HLDDLLGDVP LVVFSSATGT LGTPGQANYA AANAVADALV QRRRARGLPG VSLAWGLWSD
1561 TSELTATMDA ADVARTRRGG VLGLDAARGL ALLDAALGAD DALLVPIHLD TAALRRGADP
1621 APPLLRGLVR RARRAAGAAR QAALPLVARL AGVDAAERRR ALVELVRAEA AAVLGHGGPD
1681 GIGQDQPFRE VGFDSLTAVE LRNRLGAATG LALPATVVFD HPTSARVAEH LRELLFGAET
1741 AQAPARREVV ADDDPIAVVG MACRFPGGVA DADGLWRLVA EERDGIGPFP DDRGWDLAAL
1801 FDQDPDHAGT SYVREGGFLD GATGFDAPFF GISPREALAM DPQQRLLLEV AWETFEQAGI
1861 NPRSAHGTDT GVFAGVIYHD YGEAAGELPE GAETYRSTGT SGSVVSGRVA YALGLTGPAL
1921 TIDTACSSSL VAIHLGARAL RAGECSMALV GGVTVMSTPG GFVSFSRQRG LAPDGRCKSF
1981 SEGADGTGFS EGVGLVLLER LSDARRNGHQ VLAVVRGSAV NQDGASNGLT APNGPSQQRV
2041 IRAALDAAGL GHADVDAVEA HGTGTTLGDP IEAQAVLATY GQDREQPLWL GTLKSNLGHT
2101 QAAAGIGSVI KMIQAMRHGV LPRTLHVTEP TTAVDWGAGA VELLTRAREW PETGRPRRAG
2161 VSSFGVSGTN AHVILEQAPE PVAVEAAPEA GVLPWVLSAR TPEALREQAD RLVAHLGGES
2221 SSAAVARSLV LGRAALEERA VVVGDRARAG EALRALAEGR PSPALVTGRT GVEGRVVFVF
2281 PGQGAQWVGM GRALLDASPV FAERLRECAA ALRPYTDWDL VEVITSGGAL EDVDVVQPAS
2341 WAVMVSLAAL WRSLGVEPDA VIGHSQGEIA AATVAGWLSL QDGAKIVALR SQLIDEELTG
2401 LGGMMSVALP AEDIDLSGYE GRLWVATVNG PSATVVAGDT GALEELRRGF GEAVRTRVIP
2461 VDYASHTGHV DAIRDQLARM LADVTPRPGE IPWLSTVTGE WITPGDDDAD YWFHNLRRTV
2521 HFADGITTLL DAGHRVFVEV SSHPVLAAAV QESAEAAGDA RVAVTGTLRR DDGGWDRVLT
2581 GLAELHVRGV DVDWTRVLPE AGRAPLPTYA FQHERYWPEP ARPAAAPGGG DDALWAVIEG
2641 GDAADLAGEL AVDEDELARV LPALTSWRRR SRARSALDGW RYRVDWVPVP TSGSGLPGGQ
2701 ALSGGQALSS GQALSSGQAL SSGQALSSGQ ALSSGQALSG GPRSSGGAGL SGGQGTPGGS
2761 GSPGGAALPG GPGSPGGAAL PGRVAVVVPA NDERARAVAG GLVARGVDVT VVAAVDATRD
2821 GLAKALPDRP DAVVSLLSWD AGADEPGAPG SATAALVQAL ADRGATGPLW CATGGAVSVA
2881 GEDADPDQAA VWGLGGVLAL DLPEAFGGLV DLPRQPTDAD LDAFAAALTA PGGEDQLAVR
2941 DGRLLARRLV RDGADAPEWT PRGAVLVTGG TGGLGTHVAR WLARSGAGHV VLASRSGPAA
3001 PGAAGLAAEV EALGARCSVV ALDVADRDAV AAVLADVERD GPLTAVVHAA GAGLAPTPVV
3061 ELTAGRYADV AAGKVEGARV LDEVLADRAL DAFVLFSSGA AVWGSGGQAP YAAANAFLDG
3121 LAARRRARGL VATSVAWGGW GGGLGMIGDG DAERWARLGI RTMDPEAALR GMALAVGSGR
3181 AASVVADVDW ARFAPGYALA RERPLLRGLP EVVALLAEPD EPAAAAVDAR GALAARLTGL
3241 DAAGQDELLA DLVRAQAAAV LGFADPGAVA ADRAFKDAGF DSVTAVELRN RLGAATGLRL
3301 PPTVVFDHPK PLALARVLRA ELVPQRGDGV TAAQVAHRED AIRRVLASVP LARFEELGVL
3361 GALVDLVPAA PPAGGAATAE RDDLADLAEL DLDGLVRRAM RGTTAGND
//