LOCUS ACU37091.1 3355 aa PRT BCT 24-DEC-2013
DEFINITION Actinosynnema mirum DSM 43827 Acyl transferase protein.
ACCESSION CP001630-3117
PROTEIN_ID ACU37091.1
SOURCE Actinosynnema mirum DSM 43827
ORGANISM Actinosynnema mirum DSM 43827
Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
Actinosynnema.
REFERENCE 1 (bases 1 to 8248144)
AUTHORS Land,M., Lapidus,A., Mayilraj,S., Chen,F., Copeland,A., Del
Rio,T.G., Nolan,M., Lucas,S., Tice,H., Cheng,J.F., Chertkov,O.,
Bruce,D., Goodwin,L., Pitluck,S., Rohde,M., Goker,M., Pati,A.,
Ivanova,N., Mavromatis,K., Chen,A., Palaniappan,K., Hauser,L.,
Chang,Y.J., Jeffries,C.C., Brettin,T., Detter,J.C., Han,C.,
Chain,P., Tindall,B.J., Bristow,J., Eisen,J.A., Markowitz,V.,
Hugenholtz,P., Kyrpides,N.C. and Klenk,H.P.
TITLE Complete genome sequence of Actinosynnema mirum type strain (101)
JOURNAL Stand Genomic Sci 1 (1), 46-53 (2009)
PUBMED 21304636
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 8248144)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N.,
Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C.,
Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F.,
Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE The complete genome of Actinosynnema mirum DSM 43827
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 8248144)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N.,
Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C.,
Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F.,
Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE Direct Submission
JOURNAL Submitted (29-MAY-2009) US DOE Joint Genome Institute, 2800
Mitchell Drive, Walnut Creek, CA 94598-1698, USA
COMMENT URL -- http://www.jgi.doe.gov
JGI Project ID: 4082379
Source DNA and organism available from Hans-Peter Klenk at the
German Collection of Microorganisms and Cell Cultures (DSMZ)
(hans-peter.klenk@dsmz.de)
Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu)
David Bruce (microbe@cuba.jgi-psf.org)
Whole genome sequencing and draft assembly at JGI-PGF
Finishing done by JGI-LANL
Annotation by JGI-ORNL and JGI-PGF
The JGI and collaborators endorse the principles for the
distribution and use of large scale sequencing data adopted by the
larger genome sequencing community and urge users of this data to
follow them. It is our intention to publish the work of this
project in a timely fashion and we welcome collaborative
interaction on the project and analysis.
(http://www.genome.gov/page.cfm?pageID=10506376).
##Metadata-START##
Organism Display Name :: Actinosynnema mirum 101, DSM 43827
Culture Collection ID :: DSM 43827, ATCC 29888, NBRC 14064
GOLD Stamp ID :: Gc01024
Funding Program :: DOE-GEBA 2007
Assembly Method :: Newbler version 1.1.02.15, Phrap
Sequencing Depth :: 8.9x Sanger; 20x pyrosequence
Gene Calling Method :: Prodigal
Isolation Site :: "Blade of grass from Raritan River NJ,
USA"
Collection Date :: September 1976
Isolation Country :: USA
Latitude :: 40.491816
Longitude :: -74.322087
Oxygen Requirement :: Aerobe
Motility :: Nonmotile
Sporulation :: Sporulating
Temperature Range :: Mesophile
Temperature Optimum :: 10-30C
Gram Staining :: gram+
Biotic Relationship :: Free living
Diseases :: None
Habitat :: Soil
Phenotypes :: Nocardicin A producer
Energy Source :: Chemoorganotroph
##Metadata-END##
FEATURES Qualifiers
source /organism="Actinosynnema mirum DSM 43827"
/mol_type="genomic DNA"
/strain="DSM 43827"
/type_material="type strain of Actinosynnema mirum"
/db_xref="taxon:446462"
protein /locus_tag="Amir_3180"
/inference="protein motif:PFAM:PF00698"
/note="PFAM: Acyl transferase; short-chain
dehydrogenase/reductase SDR; Beta-ketoacyl synthase; KR
domain protein; Erythronolide synthase docking; NAD-
dependent epimerase/dehydratase; phosphopantetheine-
binding; KEGG: met:M446_0170 beta-ketoacyl synthase"
/transl_table=11
/db_xref="InterPro:IPR001509"
/db_xref="InterPro:IPR002198"
/db_xref="InterPro:IPR006162"
/db_xref="InterPro:IPR006163"
/db_xref="InterPro:IPR013968"
/db_xref="InterPro:IPR014030"
/db_xref="InterPro:IPR014031"
/db_xref="InterPro:IPR014043"
/db_xref="InterPro:IPR015083"
BEGIN
1 MSDEKKLRDY LKRALAENER VQQRLRALEA ANREPIAVVA TACRFPGGVA SPEDLWDLVA
61 RGGDVISGFP TDRGWDLDAL FDGDPEAQGR SYVREGGFLR EATEFDAAFF GISPREALAM
121 DPQQRVLLET AWEVVERAGI DPTSLRGSRT GVFTGVVYYD FATRLHPVPE GMEGHLGTGI
181 APSVISGRVA YVLGLHGPAV TLDTGCSSSL VGLHLAAQAL RSGECSLALA GGVTVMSLPG
241 AFPEFSRQRG LAPDGRCKSF ADAADGTAWS EGVGLVLLER LSDAERAGHP VLAVLRGSAV
301 NSDGASNGLT APNGVAQQRV IRSALEFAGL RPSDVDLVEA HGTGTRLGDP IEAQAILATY
361 GQDRAEPVLL GSVKSNLGHT QGAAGAAGLI KVVEALRRGE VPRTLHVDRP SGQVDWSAGN
421 AALATEHVPW PDRGRPRRAA VSAFGVSGTN AHVVLEGVPA PEVPPRDDRA LPLPLSARAE
481 PALRALAGRV GALLDGPVAL ADVAGSLADG RARLAHRAVV VAEDRAGART ALDALAAGEP
541 HPDLVTGVAD VDGRTAFVFP GQGAAWVGMG ARLLTESAVF ADSVDRCAAA LAPHTDVDLR
601 AALTGGPLDR VETVQVAAFA VAVSLAELWR AHGVEPDAVL GHSQGEIAAA CVAGALTLED
661 AALVVALRSR AVAEHLSGRG AMLSVALPPH EVGSFGLAVA AVNGPESVVL SGPVADVEAA
721 LAALTASGVR ARRVPVDYAS HSPGVEVLRE VLATALAPVR PRRARVPFLS STTGAWLEGP
781 ELDAGYWFAN LRGAVGFHAA TTTLLDAGHR AFVEVSAHPV LTGAVQDSAD GRAVVAGTLR
841 RDQGGLHRFR LSAAELHARG VPVDWNAGSG DRWRRVDLPT TPFHRQRCWP DARRAATDQP
901 GLRAANHPLL DTVVALAGGD GVVCAGSAST RAHPWLADHA VSGTALLPGT GWVELAVRAG
961 DEVGLPVLDE LVVEQPLPLG PQTTPLQVRV GPLDATARRP VELHSGSASG GEWTRHARGH
1021 LTAATPTTAG GPVEWPPPGA REVDLTGFYE RQAATGYGYG PAFRGLRRCW TRDGEVFAEV
1081 VLPGEPGAFG VHPALLDAAL HAAAVTAPEG VPVLLPFTWT GVALHATGAR ALRVRLTTPR
1141 AGELALTASD GDGRPVVTVE SLVLRPAGDL TATRTPRSLF EVVWTEAPAQ APTPTPTSPR
1201 PVLRHDVPAA SAEDAVCSVL AVVQGFLADP RHADSVLLVV TRGGEGPDGA TAATSAVHGL
1261 VRSAQAEEPG RIVLVDTDGS APDDLITALA AGPEPQLAVR AGRVTAPRLR RAAPAGAAPA
1321 APALAPEGTV LVTGGTGVLG AAVARHLVTA HGARHLLLLS RRGPDAPGAA DLVAELTGAG
1381 AHVVVRAVDV ADRAALAAAL DAVPAAHPLT GVVHLAAALD DGTITALTPE RVRRVLAPKA
1441 TAAAHLHELT SDLALFALFS SASGVLGAPG QGNYAAANAY LDGLARERHA AGLPATSIAW
1501 GLWAEATGLT GSLSGTDRER MAASGVRPLT EAEGLALFDA ALAGPAFVAA GLAGSATGQV
1561 PPVLRDVVRA TRPAAAAVRP ARTPADLLAL VRAEAASVLG HPAGEPVDAD RAFKEAGFDS
1621 LTAVELRNRL TAATGVRLPA TLVFDHPTPA ALADHLVERM GAPVAPRPER AAKAASGEPI
1681 AIVSIGCRLP GGVTSPEGLW RLLLDGVDAT TGPPDDRGWD PATPYRGGFL DGATTFDAGF
1741 FGIGPREALA MDPQQRLLLE TSWELFERAG IDPTSLRGTD TGVFAGVMNQ DYLSRAAGAP
1801 PDAEGFLSTG NSGSVVSGRL AYTYGLEGPA LTVDTACSSS LVAVHLAVRS LRSGECSLAL
1861 AGGVTVMATT GLFADFDRQG GLAADGRCKA FSADADGTAF GEGAGLVLLE RLSDARRNGH
1921 AVLAVLRGTA VNSDGASNGL TAPNGPAQQR VIRAALADAG LSPAEVDAVE AHGTGTRLGD
1981 PIEAQALLAT YGQGRGEPVL LGSVKSNLGH TQGAAGVTGL IKLVLALRHG VVPATLHAGR
2041 ASAEVDWDAG AAELATGARP WPRTGRPRRA AVSSFGISGT NAHAVLEQAP EDVDGGVVEV
2101 APAGVPRDGR ARAADLATAG ARGSEADGAA PPVGAAAGSG VTGARESEVE PGGVDPAAAS
2161 TDAAQAAAAQ SGGTAAREPE TGVAADPGGP GSTVVPADAA RAADVAGADP APLAPLVVSA
2221 RDERALAAQV ERITALPHDP VDVGWSLVTT RATLRHRAVL LDGEVVASGV PAPGGTAWLF
2281 TGQGGYRPGT GRALRARFPA FRDAFDATCA ELDRALDGRA GHPVRDVALG ERPGLDGTLH
2341 AQAAAFALQT ALAAQLGAFG LAPDAVAGHS VGEIAAAHVA GAFPLDAACA LVAERGVLMR
2401 DLRPGAMVAV EVTEEEALDA IGDLPVAVAA VNGPEAVVLS GDAEAVVAVA ARLGGRRKRL
2461 PVDRAFHSPH VDPVLDALRG LATGVPTTPL ISTTTGGPLT RVGDDHWADH ARGAVRFADA
2521 LRALVDSGVT MAVELGPDAV LTPHAAEVLA RATPTLRADH DEVASLLTAL AVLHARGAAV
2581 DWRPAFEGAG ARRVDLPTYP FQRTRYWLDA APRATATGHP LVDALDVLPG DEVLLSARWH
2641 AGRAGLGATP LAALLEPVIR AGDEVGCPVV AELHLATPVP LDAGPRHVRV RVAAPDADGR
2701 RAVTVHSRPD DEPAWTGHAT GVLAPAGHPE PQSHEAGALG AGADGHGADI PAARNDGPWP
2761 SHGADSPVGG TNGAWLPHDS GALAAGANGA RSTPDATGPA LGAELVLPDG AEPDGFGLHP
2821 ALWEALLPHP ARCADVVLHA TGATALRVRG TSPLLAVDGA GDPVVSVGGV TRAVASPRAV
2881 TRGSLFEVVE VPLPTPAPTG PSTALSADLR GATPAEALDV LQRFAAGDAP RLVVRTDRPD
2941 DPWQCAVRGL VRSAQAEEPG RILLLVQDGD GPEPPPTTRE PEVVVRDGAL RVPRLRRTAP
3001 RHDGVRALPD TVLITGGTGA LGSTLARHLA AGGVRTLVLA SRRGPTAPGA PELAADLARL
3061 GARAHLVAAD LTDRAALAAL LAEHRPGGVV HTAGVLDDGV LSALTPARLD AVLAPKALVA
3121 AHLDELTRDP ATTLFVLFSS AAGVLGTPGQ ANYAAANAFL DGLALRRRAA GLHALSLAWG
3181 PWRSGMAAGV DASRAGLHPL DDAEATALFD AALADGRTLL APLSLAATPQ DVDVPPLLRQ
3241 LVPVRRRSAR AEVAGDPVDR VRDAADHELP ELLLALVRTA TATVLGHRGP ESVDPDEPFW
3301 DTGFTSLTAV ELRNLLAERT GVRVTAAAVY EEPTPRALSA HLRAALRAPH PSGTV
//