LOCUS ACU36987.1 612 aa PRT BCT 24-DEC-2013
DEFINITION Actinosynnema mirum DSM 43827 asparagine synthase (glutamine-
hydrolyzing) protein.
ACCESSION CP001630-3013
PROTEIN_ID ACU36987.1
SOURCE Actinosynnema mirum DSM 43827
ORGANISM Actinosynnema mirum DSM 43827
Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
Actinosynnema.
REFERENCE 1 (bases 1 to 8248144)
AUTHORS Land,M., Lapidus,A., Mayilraj,S., Chen,F., Copeland,A., Del
Rio,T.G., Nolan,M., Lucas,S., Tice,H., Cheng,J.F., Chertkov,O.,
Bruce,D., Goodwin,L., Pitluck,S., Rohde,M., Goker,M., Pati,A.,
Ivanova,N., Mavromatis,K., Chen,A., Palaniappan,K., Hauser,L.,
Chang,Y.J., Jeffries,C.C., Brettin,T., Detter,J.C., Han,C.,
Chain,P., Tindall,B.J., Bristow,J., Eisen,J.A., Markowitz,V.,
Hugenholtz,P., Kyrpides,N.C. and Klenk,H.P.
TITLE Complete genome sequence of Actinosynnema mirum type strain (101)
JOURNAL Stand Genomic Sci 1 (1), 46-53 (2009)
PUBMED 21304636
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 8248144)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N.,
Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C.,
Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F.,
Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE The complete genome of Actinosynnema mirum DSM 43827
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 8248144)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N.,
Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C.,
Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F.,
Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE Direct Submission
JOURNAL Submitted (29-MAY-2009) US DOE Joint Genome Institute, 2800
Mitchell Drive, Walnut Creek, CA 94598-1698, USA
COMMENT URL -- http://www.jgi.doe.gov
JGI Project ID: 4082379
Source DNA and organism available from Hans-Peter Klenk at the
German Collection of Microorganisms and Cell Cultures (DSMZ)
(hans-peter.klenk@dsmz.de)
Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu)
David Bruce (microbe@cuba.jgi-psf.org)
Whole genome sequencing and draft assembly at JGI-PGF
Finishing done by JGI-LANL
Annotation by JGI-ORNL and JGI-PGF
The JGI and collaborators endorse the principles for the
distribution and use of large scale sequencing data adopted by the
larger genome sequencing community and urge users of this data to
follow them. It is our intention to publish the work of this
project in a timely fashion and we welcome collaborative
interaction on the project and analysis.
(http://www.genome.gov/page.cfm?pageID=10506376).
##Metadata-START##
Organism Display Name :: Actinosynnema mirum 101, DSM 43827
Culture Collection ID :: DSM 43827, ATCC 29888, NBRC 14064
GOLD Stamp ID :: Gc01024
Funding Program :: DOE-GEBA 2007
Assembly Method :: Newbler version 1.1.02.15, Phrap
Sequencing Depth :: 8.9x Sanger; 20x pyrosequence
Gene Calling Method :: Prodigal
Isolation Site :: "Blade of grass from Raritan River NJ,
USA"
Collection Date :: September 1976
Isolation Country :: USA
Latitude :: 40.491816
Longitude :: -74.322087
Oxygen Requirement :: Aerobe
Motility :: Nonmotile
Sporulation :: Sporulating
Temperature Range :: Mesophile
Temperature Optimum :: 10-30C
Gram Staining :: gram+
Biotic Relationship :: Free living
Diseases :: None
Habitat :: Soil
Phenotypes :: Nocardicin A producer
Energy Source :: Chemoorganotroph
##Metadata-END##
FEATURES Qualifiers
source /organism="Actinosynnema mirum DSM 43827"
/mol_type="genomic DNA"
/strain="DSM 43827"
/type_material="type strain of Actinosynnema mirum"
/db_xref="taxon:446462"
protein /locus_tag="Amir_3071"
/EC_number="6.3.5.4"
/inference="protein motif:TFAM:TIGR01536"
/note="KEGG: scl:sce4939 hypothetical protein; TIGRFAM:
asparagine synthase (glutamine- hydrolyzing); PFAM:
asparagine synthase; glutamine amidotransferase class-II"
/transl_table=11
/db_xref="InterPro:IPR000583"
/db_xref="InterPro:IPR001962"
/db_xref="InterPro:IPR006426"
BEGIN
1 MCGITGWVSH DSDLTTRPGA GDALAGMTAT MACRGPDDSG TWWHPDAALG HRRLAVIDLP
61 GGRQPMVLPT PRGDLCLVYS GETYNHAELR AELTALGHTW RTASDTEVVL HGYLEWGERV
121 VEHLNGMYAF AVWDSRDRTL LLARDRMGVK PLYYRPTPDG VLFGSEPKAV LAHPLAERAV
181 DDDGLRELLL PTQRPGWSLW KDLVPVEPGT TVTVSRSGIR ARTYWSLRTT PHADDRDTTV
241 ATVRELLTDT VRRQLVADVP RCVLLSGGLD SSAITGLAAA ELTEPVRTFS VDFPGQEENF
301 APDDLRPTPD APYVRDVVGL VGSDHHDVLL DPAHLTDPDT RRAVLTARDA PTGAGDLDVS
361 LHLLFKAIRA DSTVALSGES ADEVFGGYRW FHDGSAEVDG FPWMVAFRES GSGAASPYRA
421 DLMRRLDLGS YVRDEYRAAV ARVEPLEGES AREARMRVVC HLHLTRFVRM LLDRKDRMSM
481 AVGLEVRVPF CDHRLVEYAY NAPWALKTFD GREKSLLRHA TAHVLPASVR DRVKSPYPST
541 QDPGYAAALQ RQVAQVLAEP GHPVFDLVDR ERARLLVGTD PARVGPAQRF ALDRVLDLSH
601 WFDLYRPRLL AS
//