LOCUS ACU36630.1 7110 aa PRT BCT 24-DEC-2013
DEFINITION Actinosynnema mirum DSM 43827 short-chain dehydrogenase/
reductase SDR protein.
ACCESSION CP001630-2656
PROTEIN_ID ACU36630.1
SOURCE Actinosynnema mirum DSM 43827
ORGANISM Actinosynnema mirum DSM 43827
Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
Actinosynnema.
REFERENCE 1 (bases 1 to 8248144)
AUTHORS Land,M., Lapidus,A., Mayilraj,S., Chen,F., Copeland,A., Del
Rio,T.G., Nolan,M., Lucas,S., Tice,H., Cheng,J.F., Chertkov,O.,
Bruce,D., Goodwin,L., Pitluck,S., Rohde,M., Goker,M., Pati,A.,
Ivanova,N., Mavromatis,K., Chen,A., Palaniappan,K., Hauser,L.,
Chang,Y.J., Jeffries,C.C., Brettin,T., Detter,J.C., Han,C.,
Chain,P., Tindall,B.J., Bristow,J., Eisen,J.A., Markowitz,V.,
Hugenholtz,P., Kyrpides,N.C. and Klenk,H.P.
TITLE Complete genome sequence of Actinosynnema mirum type strain (101)
JOURNAL Stand Genomic Sci 1 (1), 46-53 (2009)
PUBMED 21304636
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 8248144)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N.,
Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C.,
Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F.,
Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE The complete genome of Actinosynnema mirum DSM 43827
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 8248144)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N.,
Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C.,
Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F.,
Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE Direct Submission
JOURNAL Submitted (29-MAY-2009) US DOE Joint Genome Institute, 2800
Mitchell Drive, Walnut Creek, CA 94598-1698, USA
COMMENT URL -- http://www.jgi.doe.gov
JGI Project ID: 4082379
Source DNA and organism available from Hans-Peter Klenk at the
German Collection of Microorganisms and Cell Cultures (DSMZ)
(hans-peter.klenk@dsmz.de)
Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu)
David Bruce (microbe@cuba.jgi-psf.org)
Whole genome sequencing and draft assembly at JGI-PGF
Finishing done by JGI-LANL
Annotation by JGI-ORNL and JGI-PGF
The JGI and collaborators endorse the principles for the
distribution and use of large scale sequencing data adopted by the
larger genome sequencing community and urge users of this data to
follow them. It is our intention to publish the work of this
project in a timely fashion and we welcome collaborative
interaction on the project and analysis.
(http://www.genome.gov/page.cfm?pageID=10506376).
##Metadata-START##
Organism Display Name :: Actinosynnema mirum 101, DSM 43827
Culture Collection ID :: DSM 43827, ATCC 29888, NBRC 14064
GOLD Stamp ID :: Gc01024
Funding Program :: DOE-GEBA 2007
Assembly Method :: Newbler version 1.1.02.15, Phrap
Sequencing Depth :: 8.9x Sanger; 20x pyrosequence
Gene Calling Method :: Prodigal
Isolation Site :: "Blade of grass from Raritan River NJ,
USA"
Collection Date :: September 1976
Isolation Country :: USA
Latitude :: 40.491816
Longitude :: -74.322087
Oxygen Requirement :: Aerobe
Motility :: Nonmotile
Sporulation :: Sporulating
Temperature Range :: Mesophile
Temperature Optimum :: 10-30C
Gram Staining :: gram+
Biotic Relationship :: Free living
Diseases :: None
Habitat :: Soil
Phenotypes :: Nocardicin A producer
Energy Source :: Chemoorganotroph
##Metadata-END##
FEATURES Qualifiers
source /organism="Actinosynnema mirum DSM 43827"
/mol_type="genomic DNA"
/strain="DSM 43827"
/type_material="type strain of Actinosynnema mirum"
/db_xref="taxon:446462"
protein /locus_tag="Amir_2695"
/inference="protein motif:PFAM:PF00106"
/note="PFAM: short-chain dehydrogenase/reductase SDR;
Beta- ketoacyl synthase; Male sterility domain; Acyl
transferase; KR domain protein; phosphopantetheine-
binding; NAD-dependent epimerase/dehydratase; KEGG:
met:M446_0170 beta-ketoacyl synthase"
/transl_table=11
/db_xref="InterPro:IPR000169"
/db_xref="InterPro:IPR001509"
/db_xref="InterPro:IPR002198"
/db_xref="InterPro:IPR006162"
/db_xref="InterPro:IPR006163"
/db_xref="InterPro:IPR013120"
/db_xref="InterPro:IPR013968"
/db_xref="InterPro:IPR014030"
/db_xref="InterPro:IPR014031"
/db_xref="InterPro:IPR014043"
BEGIN
1 MSQPENAEPA TTAAPLARLA GLPPAEQERV LLGVVHEQVV AVLKAALPDA PDRVAPERPF
61 KELGFDSLAA VDLHRRLSAA TGLELPVTLV FDHPTPLAVA RLARRLALGE RAVPVVREPV
121 ARRERDDEPI AVVGIGCRYP GGSDTPEKLW DVVAGGRHVI SDFPEDRGWD LDALFDPEPG
181 KPGKSYVRKG GFLTEAAEFD AAFFGISPRE ALAMDPQQRL VLETAWEALE HAGVDPLSLR
241 GSRTGVYIGV EPQEYGPRLH QAPEGLDGYL LTGNAPSVVS GRLAYSLGLE GPTVSIDTAC
301 SGSLVALHLA VRALRDGDCT AALAGGVAVM VHPGAFTAFS TQRGLAPDGV CKPFAAAADG
361 TGWAEGVGVV VLERLSDALR AGHRVLGVIK GTAVNQDGAS NGLTAPSGPA QQRVILQALA
421 DAGLSTSDVD TVEAHGTGTR LGDPIEAQAL LATYGQDRET PLWLGSIKSN IGHSQAAAGV
481 AGLIKVVYAM RERVLPRTLH VDAPSPHVDW SAGDIELLTE QRPWDSPDRP RRAGISSFGV
541 SGTNAHVVIE EPPAAPPVAP AKPVKAAKAT RKSQKAKAAA PVPVPLLLSG RGEAALRGQA
601 TRLAEFLAGD AAATPDLVDT AHELALRRAA LEHRAVLHAT DRADALRGLA ALAAGQDAPG
661 LVRGAATAGP TAFLFTGQGS QRLSAGRELY RAHPAFAKAL DAACGWLDLQ LDRPLGEVMF
721 AEPGTEAAAL LDRTAYAQSA LFALETALYR LVESWGLRPD YVAGHSLGGI TAAHVAGVLS
781 LEDAALLVGA RGRLMQALPE GGAMVSVRAP ESEVLPLLEG REAEVRVAAV NGPAATVISG
841 VETAVLEVAG VLADRGVKTK RLTVSHAFHS PLMEPALADF RRVAEVMTYS APTIPVVSDV
901 TGELATAEQL RSPEYWVDHV RQAVRFRDVV AELAARGVAT FLELGPDAVL TAMAQDCVAE
961 IDTDTDTDFA FAALLRRDRD EVAEVRAAVA LAHTRGQSVR WAELLPQGSG ARVDLPRYAF
1021 QRERYWLTGG SSAGGAVELG QLAADHPLVG AVVGLAGGDR VVLTGRVSPR THPWLADHVI
1081 SGSVLLPGTA FVELAVRAGD EVGCAVLEEL TLEAPLVLPE RGGVVLQVVV DAPDDGGRRT
1141 VAVHSRPEHA PEDAGWTRHA GGVLSAQAPE PGERLETWPP RDAEPVDAST AYADLAALGY
1201 GYGPVFQGLR KAWRRGTGPS AEVYAEVALP ESVAASAASF GLHPALLDAA LHAVDVAGTA
1261 PGDPGEVRVP FAWNDVVLHA TGASAVRVRI SPAGSDAVSL TITDPAGAPV AAVGSFVSRA
1321 VTAEQLAAAR REHHESLLRQ EWVSRPVPAG QVRPAAVLGA GHADLAALGA ALTSGATVPE
1381 TVVYHVPVST GDVLVDVRAA LDSVLTVVRQ WLADERFGPS RLVVVTRGVR DADLAHTPVW
1441 GLVRAAQAEN PDRFTLVDLD DAPESAAQLL PAVATGEPEL SVRDGKVSVP RLARVPVEEP
1501 AADPWAGEGT TLVTGGTGGL GALVAKHLAT RGARRLVLTS RRGPDAPGAA DLVAELADLG
1561 ATARVVACDV ADRAAVADLL AGITDLTAVV HTAGVVADGT VGALTAERVD EVLRPKADAA
1621 WHLHELTRGR DLTAFVLYSS LAALLDNPGQ GNYAAANLFL NALAERRRAE GLPATALTWG
1681 LWLGESGMGS VLDTTALHRI EASGMPGLTP EENLALLDAA LTTTEPVLAP VRLDPTALRA
1741 RPDGVPALLR VLAPTAARRA AGGAAQSGGD SLGARLAELP EADRDRVLLD LVRAHVAAVL
1801 GHGGGDAIEP KRAFSEIGFD SLAAVELRNR LTTATGLRLP ATLVFDYPTP LALVGHLKDK
1861 ALGDRAAAAP VQRATATAVD DEPIAVVGMS CRFPGGVTTP EELWRLVADG VDGISRFPDD
1921 RGWDVDSLYD PEPGKPGRTY SVEGGFLDDA LDFDAEFFGI SPREATAMDP QQRLLLETAW
1981 EALERAGIDP TSLKGSPTGV FAGIMYHDYA LRLKDIPEDL AGYLGNGSLA SVASGRVSYV
2041 LGLEGPAVSV DTACSSSLVS IHLAAQALRG GECSLALAGG VTVMATPDTF LDFSLQRGLA
2101 ADGRSKSFSA DADGTAWGEG VGVLVLERLS DARRNGHEVL AVVKGSAINQ DGASNGLTAP
2161 NGPSQQRVIR AALSMAGLSH TEVDAVEAHG TGTRLGDPIE AQALLATYGQ DRTEPLWLGS
2221 IKSNVGHTQA AAGVAGVIKM VMAMREGVLP RTLHVGERTP QVDWSEGAVE LLTQARPWPE
2281 TGRPRRAGVS SFGISGTNAH VVLEQAPAAE PVAPVEPVEP QSAESQPVEQ RPSGLVPWVL
2341 SARTGDALRE QAARLREHVG SASPRDVGWS LATTRAALER RAVVVGEDAG QLGAGLSALA
2401 SGEAAASVVD GEALSDPRVV FVFPGQGSQW AGMAVELLDS SPVFAERMRE CAAALSPHVD
2461 WNLVDVVRDA PGTPPLNRVD VLQPVLWAIT VSLAELWKHH GVRPDAVVGH SQGEIAAAVV
2521 SGALSLQDGA AIVALRSVLI GEELSGRSGM LSIQLPLDEV LPRLRGGLTI GALNGARSVV
2581 VSGDPENLAA LQAELTAEGV RARPVKIDYA SHSPHVEVIR ERLLAVIADV APRAATTPFC
2641 STVTGGFLDG ATLDADYWYS NLREKVDFVG ATRALLGSGH TVFVECSPHP VLPSAVQETA
2701 DEDDREVVTV PTLRREDGGP RRLLLSLAQA WTRGVDVDWT TLLTGPAAPR RVALPTYAFQ
2761 HRRFWLDATD GPGDVSSAGL GATGHPLLGA TTTTAGNDGV LLTGRLALDT HPWLADHAAS
2821 GVVLLPGTAF VELAVRAGDQ VGCDRVDELT LEAPLVLPAR GGTVLQVAVG APDATGRRAV
2881 SVHSRPEDAD SSWTRHASGF LDHAPRTSPE RLTAWPPPGA AKLDVDALYA ELAAGGYAYG
2941 PVFRAVRAAW RQGDVVFAEV ALPEHADAAG FGLHPALLDA ALHATGAREG GAEDGLIDLP
3001 FAWTGVTLHA SGATGLRVRL TPEGGGAVSL LLADPTGAPV ATVDALVTRP IPAEALTGGS
3061 GQDSLYRVDW TPVPAAGPVT GQVVVLDGPD LSALEQVPDW VVLPVSGQAA RSTVDDVLSV
3121 LREWIADERF AAARLALVTT GGVPAAGHAV DPVVAPVWGL VRAAQAENPD RFALLDLDVA
3181 LSDSPENLAG AVTTPLGLGE PEVAVRGDAL LVPRLARATD RPRAPFAWTA EDTVLITGGT
3241 GGLGALLAGH LAGQGVRQLV LTSRRGPDAP GATELVARLA DLGATARVVA CDVADRDSVA
3301 ELLADVDGLT AVVHTAGALA DGLVSAMTPE QVDAVWLPKA EGARNLHELT SDLRAFVVFS
3361 SAAGVVDGTG QGNYAAANTY LDALVEQRRA TGLPGTSIAW GLWEHRSGMT GHLTEGDLAR
3421 MARSGVRGLP AAEGLALFDA ALALDAPLLV PVRLDLAALR TRGEELPRLF HGLVRPTRRA
3481 ATAAGADRTP RDELLGLSAE DRGRVLLDLV RSHVAAVLGH GSGAEIEPRR AFGELGFDSL
3541 AAVELRNRLT TATGLRLPAT LVFDHPTPVA LVAHLSESLG GAAPSAPTPV AATRADDGDP
3601 IAIVAMGCRY PGDVTTPEEL WKLVADGVDA IGGFPVDRGW DTDGIYDPEP GKPGRTYVRE
3661 GGFLNDALGF DAGFFGISPR EALAMDPQQR LLLETAWEVI ERAGIDPTSL KGTPTGVFAG
3721 VMYHDYGSRL KEVPEDLAGY LGNGSIASVL SGRVSYVLGL EGPAVSVDTA CSSSLVTIHL
3781 AAQALRAGEC SLALAGGVTV MSAPDTFVDF SLQRGLARNG RSKSFSSDAD GTGWGEGVGL
3841 VLLERLSDAR RNGHEVLAVL RGSAVNQDGA SNGLTAPNGP SQQRVIRAAL ANAGLSHTEV
3901 DAVEAHGTGT PLGDPIEAQA LLATYGQDRE IPLLLGSIKS NFGHTQAAAG VAGVIKMVMA
3961 MREGQVPATL HVSERTDQVD WSEGAVELLT EARPWPETGR PRRAGVSSFG ISGTNAHIVL
4021 EQAPEPATRA VPAPEAVAPE AIEPDAAVPT GALLPLPVSG RGADAVRAQA GLLAAHLAAH
4081 PDLAPLDVAH SLVTTRAALE QRAVVVGEDL ERLTAGLSAL ASGEAAASVV DGEVLSDPRV
4141 VFVFPGQGSQ WVGMAAELLD ASPVFAARMA ECAQALSEFV DWDLIAVARG DEDAPSLERV
4201 DVVQPVLWAM MVSLAELWRA NGVRPDAVVG HSQGEIAAAV ASGALSTRDG AAIVALRSLA
4261 IAEDLAGKGG MLSVQLPVDE VRPRLAEGLS IAVVNGARSV VVSGDPAGLD ALQAELRAEG
4321 ARAKRVPVDY ASHSAHVESI RERLLDVLAD LSPRTSTTPF CSTVTAEFTD TTTLDAGYWY
4381 TNLRETVDFV GATRTLLAGG HAVFVECSPH AVLTSAVLET AEEDGREVAA IGSLRRDDGG
4441 PRRVLLSLAQ AWTRGVDVDW TGLLADRATA AGATPRRIAL PTYAFQHRRY WLDATSATGD
4501 VTSAGLAATA HPLLGATIAV AGADEVLLTG RLAADTHPWL AHHAFAGTTL LPGTAFVELA
4561 IRAGDQVGCD RVDELTLEAP LALPERGGVH VQLRIGDPDR DGGRELTVHS RAEGSEAPWT
4621 RHATGRLIRS ETTPDFDLVA WPPPGAQPVD LAGAYPALDA QGYGYGPAFQ GLRAAWRLGD
4681 EVFAEVELPE DVDPAGYGLH PALLDAALHP VLVVESGEDK SPQLPFSWTG VSLFAEGARA
4741 LRVRLTPTGG DGLAIRVADQ AGAPVAQVAD LLSREVVVPS AGVPDALFRI DWTKVEAAPT
4801 TGKLLVLGDA LGKYVPKVPD LASIGADVPE WVVLPIGSAD TSRSAVDLAL ATAQEWLADD
4861 RFASSRLVVL TRGGAAVGDV PVDPAVTAVH GLIRAAAAEN PDRFALVDTD GWPVHWDGLL
4921 AALGSGEPEV AVRGDDLLAP RLVRATPEGP AFAWTAEDTV LVTGGTGGLG AVLARHLVER
4981 GVRQLVLTSR RGPDAPGATE LVAELADLGA TARVVACDVA DRDSVAELLA GVDGLTAVVH
5041 TAAVLDDGLV TGLTPERLDA AWNPKAEGAR HLHELTGDLR AFVLFSSAAG VYDGAAQGNY
5101 AAANTYVDGL AQARRAAGLP GVSLAWGFWS QRSGMTDHLS DVDLARMGRS GVTGLSTEEG
5161 LALFDASLAS AEPLLVPMRL DLAALRERDA LPALLKSLVR KQVRRSGVTG EEDGGSALTR
5221 RLRALPPADR TRVVLDLVRS HVAAVLGHDG AASVDPRRAF RDLGFDSLAA VELRNRLVAA
5281 TGLRLPATLV FDHPNPELLA ALLETELVGD AEPAADLPVA PTRAVDDDPI AVVGIGCRFP
5341 GDVTTPEDLW RLLDGGVDAI SAFPTDRGWD LDALFDPEPG KVGKSCSLEG GFLHDAADFD
5401 AGFFGISPRE ALATDPQQRL LLETAWEALE RAGIDPVSLR GSATGVFAGV MYNDYATRLR
5461 SVPEDLAGYV GNGSLGSVAS GRVSYVLGLE GPAVSVDTAC SSSLVSLHLA AQALRAGECS
5521 LALAGGVSVM ATPDTFVDFS LQRGLAPGGR AKSFSADADG TAMSEGVGML VLERLSDARA
5581 NGHEVLAVLR GSAVNQDGAS NGLTAPNGPS QQRVMRSALS VAGLRPSEVD AVEAHGTGTV
5641 LGDPIEAQAV IATYGQDRAE PLWLGSIKSN IGHTQAAAGV AGVIKMIMAI RAGVLPRTLH
5701 ADQRTDRVDW SGGAVELLTE SRPWPETDHP RRAGVSSFGI SGTNAHVIVE QAPTAPASTP
5761 SGQDAPTRAT PAPIVLAAAD EGALRAQAEN LLALLADGVD RTDLAHATAT TRAALEHRAA
5821 IVPGDPAEVL KSLEALATTG SGPGVVRGAR STGALALLFT GQGAQRAGMG RELRAAWPVF
5881 AEAFDEACAL LDAELDTPLA QVLDGDDPDL VQQTRYSQPA LFAFEVALHR LLQSWGVRAD
5941 LLAGHSIGEL AAAHVAGVFS LADAARLVAA RGRLMQSMRP DGGMVSVLAT EERVRADLVG
6001 LAGRVDVAAV NGPASTVVSG DADALADLVA TWKAEGLKTR ALAVSHAFHS PHTDEVLGAF
6061 REVATTIAYA EPTTPVVSTL TGRLAEPGEL TDPEYWVRHV RGAVRFHDAV RELESRGATT
6121 FLEVGPDAVL TALGRDCLLD EAAALLTATQ RADHPETTAL AAAVGALFVR GANPDWAALF
6181 AGSGARPVDL PTYPFQRRRY WLDATVGGVG SGAGHPLAGE VITTAEGDGV LLTGRLSPRT
6241 HAWLADHTVS GEVLLPGTAF VELAVQAGDQ VGCGRVEELT LQSPLVLPER GGVVLQVAVG
6301 GPDESGRRTV TAHSRPDAEG AQWTRHATGS LVPDTTIATT DADLTGEPGR WAPPDATELD
6361 LTGHYAELAA RGYGYGPAFQ GLRAAWRRGE EVFAEVALPE EVDASGYGLH PALLDATLHT
6421 AELLEDGPGE LTVPFAWKDV VLRATGASAL RVRLTRTGPT AYSVALADPD GAPVAEVGSL
6481 AVRPLPAAEG PSVGDALFRL DWTPVLGAAD TPARLAVLGA PGRFPAEVAY PDLDAVAAAG
6541 PQGPDSVLVP LRTTGDDPAA AGRAAVEGLL PVLRRWAEDE RFAGKRLVVV TTGAVSVDGE
6601 DSPGLAHAAL WGLLRSAQVE HPDLVGVVDV DGDPASDLAL PAALTAGEPQ LAVRGGRAFV
6661 PRLVRAGLPA TAPDFGTGPV LVTGGLSGVG ATVARHLVTA RGVTELLLTS RRGQDAPGAA
6721 ELVAELTGLG ASVEVAACDV ADRDAVAALL EGRELSAVVH SAGVVDDALL SGLTPEQVAA
6781 VWRPKAEAAW RLHELTRGHD LTAFVLFSSL VSVVGGAGQS AYAAANGFLD ALAAHRRSLG
6841 LPATSVAWGL WTGVGSAMTG ELGERDLRRM ASSGVLGLRP AEGLALLDAA VASDRADLVA
6901 ARLDLAAVRQ ASGAVPPVFR ALVRGAVRRV AGTGAPTSGP SVAEQVAALP PARRRDHLVE
6961 LVRGLVAGVL GHDGAHAVDA GKGFLELGFD SLAAVELRNR VAEVVGVRLS ATLVYDHPTP
7021 AAVAELLLSE LGGAEESAPS LEAELARLET ALETAAPDEE ERVRVESRLR ALVARWSGAG
7081 GAVAEAADVL DAVTADELFD ILDEELEATD
//