LOCUS ACU36557.1 790 aa PRT BCT 24-DEC-2013 DEFINITION Actinosynnema mirum DSM 43827 major facilitator superfamily MFS_1 protein. ACCESSION CP001630-2583 PROTEIN_ID ACU36557.1 SOURCE Actinosynnema mirum DSM 43827 ORGANISM Actinosynnema mirum DSM 43827 Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae; Actinosynnema. REFERENCE 1 (bases 1 to 8248144) AUTHORS Land,M., Lapidus,A., Mayilraj,S., Chen,F., Copeland,A., Del Rio,T.G., Nolan,M., Lucas,S., Tice,H., Cheng,J.F., Chertkov,O., Bruce,D., Goodwin,L., Pitluck,S., Rohde,M., Goker,M., Pati,A., Ivanova,N., Mavromatis,K., Chen,A., Palaniappan,K., Hauser,L., Chang,Y.J., Jeffries,C.C., Brettin,T., Detter,J.C., Han,C., Chain,P., Tindall,B.J., Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C. and Klenk,H.P. TITLE Complete genome sequence of Actinosynnema mirum type strain (101) JOURNAL Stand Genomic Sci 1 (1), 46-53 (2009) PUBMED 21304636 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 8248144) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE The complete genome of Actinosynnema mirum DSM 43827 JOURNAL Unpublished REFERENCE 3 (bases 1 to 8248144) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE Direct Submission JOURNAL Submitted (29-MAY-2009) US DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4082379 Source DNA and organism available from Hans-Peter Klenk at the German Collection of Microorganisms and Cell Cultures (DSMZ) (hans-peter.klenk@dsmz.de) Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu) David Bruce (microbe@cuba.jgi-psf.org) Whole genome sequencing and draft assembly at JGI-PGF Finishing done by JGI-LANL Annotation by JGI-ORNL and JGI-PGF The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##Metadata-START## Organism Display Name :: Actinosynnema mirum 101, DSM 43827 Culture Collection ID :: DSM 43827, ATCC 29888, NBRC 14064 GOLD Stamp ID :: Gc01024 Funding Program :: DOE-GEBA 2007 Assembly Method :: Newbler version 1.1.02.15, Phrap Sequencing Depth :: 8.9x Sanger; 20x pyrosequence Gene Calling Method :: Prodigal Isolation Site :: "Blade of grass from Raritan River NJ, USA" Collection Date :: September 1976 Isolation Country :: USA Latitude :: 40.491816 Longitude :: -74.322087 Oxygen Requirement :: Aerobe Motility :: Nonmotile Sporulation :: Sporulating Temperature Range :: Mesophile Temperature Optimum :: 10-30C Gram Staining :: gram+ Biotic Relationship :: Free living Diseases :: None Habitat :: Soil Phenotypes :: Nocardicin A producer Energy Source :: Chemoorganotroph ##Metadata-END## FEATURES Qualifiers source /organism="Actinosynnema mirum DSM 43827" /mol_type="genomic DNA" /strain="DSM 43827" /type_material="type strain of Actinosynnema mirum" /db_xref="taxon:446462" protein /locus_tag="Amir_2622" /inference="protein motif:PFAM:PF07690" /note="PFAM: major facilitator superfamily MFS_1; Riboflavin kinase; flavin reductase domain protein FMN- binding; KEGG: xfa:XF1765 drug:proton antiporter" /transl_table=11 /db_xref="InterPro:IPR001411" /db_xref="InterPro:IPR002563" /db_xref="InterPro:IPR007114" /db_xref="InterPro:IPR011701" /db_xref="InterPro:IPR015865" BEGIN 1 MSTSTPRGGD WLALASLCLG FFLLLVDSTI ISVALPALMA DLGADEGTAI WVNSSYLFAY 61 AVPLLVAGRL GDRYGARAVY LAGLVLFILA SLACGLVSDP LALVAWRVVQ GLGAALMTPQ 121 SMTIIRRLFA FPALGVAMGV WASVGGVASV SGPLLGGVLV GTWGWESIFL VNVPIGLLAL 181 AAAWVWLPRA EPLRTTIPVT GVLVSGLGVF GVVAGIHGGV LPPWVPGWAA VLVGLVLVGC 241 VVFAQRGEPD RALVPVPLFR DRGFVMASVG AAMSSFTVGA AMIPVMLHLQ GARGLDSMSA 301 ALVLIPMGVV CAATAPFAGR SVSARGPRFT ALIGSISLAV SVLLAAVLVA ASAPIWVFAA 361 ALTVFGLANA FVWAPMSVAA LNSAPDRLMG AASGAFNAIK QVGAVVGSAV TAALLAVTSQ 421 GAALAALGVA ALVCVLASAL LGRNAVEPLG RVSGVVVHGA GRGKDLGFPT ANLDFDASTR 481 VPEDGVYAGR FTLLDGPDAP VVLPALVSIG SNETFDGSAR TVEAHVLDFD GDLYDRRAEV 541 ELESWIRGQV GFSSVEALVE QMRRDEVVGR AHFSSSGPAG SVASSAPASA ADRWNDRVGG 601 EEPRSGVLVA ERAGGPVTEP YTDDVVALRQ AFSAFPSGVA AIAALVDGEP VSLVVSSFSV 661 GTSQEPPLVL FAVQLSSTTW PVLSTAPLLG VSVLGEGHAG KVRQLASTKD RENRFGGLET 721 AVSDSGAVFL EGAPLWLECA VEHTYPAGDH EIVVLRVRGL RSDLAQRPLV WHRSAFTTLA 781 DERERERVAG //