LOCUS ACU36431.1 2386 aa PRT BCT 24-DEC-2013
DEFINITION Actinosynnema mirum DSM 43827 Erythronolide synthase protein.
ACCESSION CP001630-2457
PROTEIN_ID ACU36431.1
SOURCE Actinosynnema mirum DSM 43827
ORGANISM Actinosynnema mirum DSM 43827
Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
Actinosynnema.
REFERENCE 1 (bases 1 to 8248144)
AUTHORS Land,M., Lapidus,A., Mayilraj,S., Chen,F., Copeland,A., Del
Rio,T.G., Nolan,M., Lucas,S., Tice,H., Cheng,J.F., Chertkov,O.,
Bruce,D., Goodwin,L., Pitluck,S., Rohde,M., Goker,M., Pati,A.,
Ivanova,N., Mavromatis,K., Chen,A., Palaniappan,K., Hauser,L.,
Chang,Y.J., Jeffries,C.C., Brettin,T., Detter,J.C., Han,C.,
Chain,P., Tindall,B.J., Bristow,J., Eisen,J.A., Markowitz,V.,
Hugenholtz,P., Kyrpides,N.C. and Klenk,H.P.
TITLE Complete genome sequence of Actinosynnema mirum type strain (101)
JOURNAL Stand Genomic Sci 1 (1), 46-53 (2009)
PUBMED 21304636
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 8248144)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N.,
Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C.,
Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F.,
Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE The complete genome of Actinosynnema mirum DSM 43827
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 8248144)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N.,
Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C.,
Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F.,
Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE Direct Submission
JOURNAL Submitted (29-MAY-2009) US DOE Joint Genome Institute, 2800
Mitchell Drive, Walnut Creek, CA 94598-1698, USA
COMMENT URL -- http://www.jgi.doe.gov
JGI Project ID: 4082379
Source DNA and organism available from Hans-Peter Klenk at the
German Collection of Microorganisms and Cell Cultures (DSMZ)
(hans-peter.klenk@dsmz.de)
Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu)
David Bruce (microbe@cuba.jgi-psf.org)
Whole genome sequencing and draft assembly at JGI-PGF
Finishing done by JGI-LANL
Annotation by JGI-ORNL and JGI-PGF
The JGI and collaborators endorse the principles for the
distribution and use of large scale sequencing data adopted by the
larger genome sequencing community and urge users of this data to
follow them. It is our intention to publish the work of this
project in a timely fashion and we welcome collaborative
interaction on the project and analysis.
(http://www.genome.gov/page.cfm?pageID=10506376).
##Metadata-START##
Organism Display Name :: Actinosynnema mirum 101, DSM 43827
Culture Collection ID :: DSM 43827, ATCC 29888, NBRC 14064
GOLD Stamp ID :: Gc01024
Funding Program :: DOE-GEBA 2007
Assembly Method :: Newbler version 1.1.02.15, Phrap
Sequencing Depth :: 8.9x Sanger; 20x pyrosequence
Gene Calling Method :: Prodigal
Isolation Site :: "Blade of grass from Raritan River NJ,
USA"
Collection Date :: September 1976
Isolation Country :: USA
Latitude :: 40.491816
Longitude :: -74.322087
Oxygen Requirement :: Aerobe
Motility :: Nonmotile
Sporulation :: Sporulating
Temperature Range :: Mesophile
Temperature Optimum :: 10-30C
Gram Staining :: gram+
Biotic Relationship :: Free living
Diseases :: None
Habitat :: Soil
Phenotypes :: Nocardicin A producer
Energy Source :: Chemoorganotroph
##Metadata-END##
FEATURES Qualifiers
source /organism="Actinosynnema mirum DSM 43827"
/mol_type="genomic DNA"
/strain="DSM 43827"
/type_material="type strain of Actinosynnema mirum"
/db_xref="taxon:446462"
protein /locus_tag="Amir_2493"
/EC_number="2.3.1.94"
/inference="protein motif:PRIAM:2.3.1.94"
/note="PFAM: Beta-ketoacyl synthase; Acyl transferase; KR
domain protein; short-chain dehydrogenase/reductase SDR;
2- nitropropane dioxygenase NPD;
phosphopantetheine-binding; KEGG: sus:Acid_1972
beta-ketoacyl synthase"
/transl_table=11
/db_xref="InterPro:IPR002198"
/db_xref="InterPro:IPR004136"
/db_xref="InterPro:IPR006163"
/db_xref="InterPro:IPR013968"
/db_xref="InterPro:IPR014030"
/db_xref="InterPro:IPR014031"
/db_xref="InterPro:IPR014043"
BEGIN
1 MRVVRSELVI ALNPVHRSAP RLTAAAARAG ALAVLELPAD VDGAVRALAD TARWTPGRFG
61 VRVRPDSVVP ALPEAAEVVL LADPARSPAE FADREVLVEV TDLAEARAAL AAGAVGLVAR
121 GRESGGRVGE LSTFVLLQQL LAEVRDVPVW AAGGIGPHTA AAAVAGGAAG VLLDTQLALY
181 DDAGLPVATT EALRGLDGSE TAVHQGVHFL HRRGPGATAL PEDPAALLHH DPARGLLPVG
241 QDVALATAFP RRWPTVAAAV RGVRDAITGA FADDAARTAL LGDQSALRTA GEARAENPQG
301 AGTASTATTP PGDRPDARSS AEPPALQRES ALGTALPVVQ GPMTRVSDSP AFAAAVADAG
361 GLPFVALALS GADQTRALLE RTRAQLGGKP WGVGILGFAA EDVRAAQLAV VEEVRPSHAI
421 IAGGRPAQAA ALEAKGVSTF LHVPSPGLLR QFLAAGARKF VFEGAECGGH VGPRNSFPLW
481 ESQVDVLLEH GAGAGTAVLF AGGVHDARSA AMVAALAAPL VRAGVEVGVL MGTAYLFTAE
541 AVDAGAIGEV FQRHVVTATR TDLLQTAPGH ATRCVASGFT GDFAALRADL ARDGVPDREA
601 WERLERFNVG RLRVASKGVE RVGDELRDVD HDRQAAEGMF MAGEVSVLRS ATTTIAALHA
661 DVTAGSAAHL AERGESVRAA TARRTAPEPK PLRVAVVGMA CMFPGAPDLP SFWANVLAGR
721 DSVTEVPPER WDPELYYDPD GDGERTPSKW GGFLPPIGFD PLAYGIPPAS LGAVEPVQLL
781 ALEASRRALV DAGYPHASTD HSRTSVVFGA ESGSDLSGAT TLRTTLPAYL GSVPPALDAQ
841 LPKLTEDSFP GVLANVIAGR VANRLDLGGA NYTVDAACAS SLTAVDVACK ELVTGSSDLV
901 LCGGADLHNG INDFLMFASV HALSPSGRSA TFDHAADGIA LGEGVACVVL KRLEDAERDG
961 DRVYAVVDGV GSASDGRALG LTAPRPEGQR AALTRAYRAA GISPAQVGLV EAHGTGTVVG
1021 DRTELRTLTE VFTEAGARPG SCAVGSVKSQ IGHTKCAAGL AGLIKTALAL HHGVTPPTLH
1081 LTRPNPAWDR DTSPFAFHSA ARPWAADPAD RVAGVSAFGF GGTNFHVVLR AHDQPPARHA
1141 LQEWPVELFL LRGNDSGAAI VERADRHPGA RLRDLARFAA ARADVAAGRG EPVRTAILAG
1201 SVAELRAKIA GTDAEGVFTA DAAEPGKLAF LFPGQGSQKP GMLAELLVAF PELHDHLRLG
1261 ARWADAMYPP TAFDEETAAL QRERVTDTAT AQPALGVVEL AATELLATFG VRPDLVAGHS
1321 YGELAALAAA GVFTPADLLT ASAARAEAIL DQVRSGDPGT MVAVTAPADV VERLVDGLPV
1381 VAANRNSPKQ TVLSGPTAAI REAEERLRAA KLGTKAIPVA CAFHSPLVAG AGEGFRRVLD
1441 TMPLRAPEIP VWANRTAAPY PADADAVRAE LAAQIGAPVR FAEQLEAMHD AGARVFVEVG
1501 PGRVLRGLVR AVLGDRPHRV VTLDGGLTGF LTALAVLAVS GADVLAGPLV RGRDAVDPAD
1561 APPVPGWTVD GHLVRRADGS IQPGALRPAH RVTAELIVPE QQAQQQTGTP DALVAEFLRT
1621 SREMISAQRD VLLTYLGAAG VPAPVVLHQV AQPVTAAAVA RPVVEQAVVA QAAVAVVRSA
1681 PVVDVLRAVV EVVAERTGYP VEMVEPDLDL EADLSVDSIK RVEIAGELAQ KLGLALDADS
1741 GLDDLAAART AGALADALRT RLPDAAPVAP VAVAAVVASG PDVLRTIVEV VAERTGYPVE
1801 MVEPELDLEA DLSVDSIKRA EIAGEVATRL GLDVGDGSRV EELSAARTAA AIAALITGAA
1861 SAAIPGAATT SATPAAALTA VTAAGAPAPS TGAPAAASTG VLSAARGGAP AAAPTPAEAT
1921 VIAPKRLVFR EVPLPLPATT DLTGARVHLV GVEQLTGADR EIPARLAALG ATLTSTPAGA
1981 VVVHLGALDP AEPPLPEEFP RFQAILTAGP TALLAVDRRG EGSVTGLRGF FRTLAREYPD
2041 LPCALLESAD PVAAIAAEIT TPSGHPVVLH GGNSRHALEL AFEDLGPVAS SGAGPAGDGT
2101 AEALAAGLDR DAVVLLVGGA RGITARFAKT LAAASRCRLE LVGRTPLPSG PEDPDLAAAT
2161 DRTALRGAFL SRGMTSPAEV ERAVSAALAA REVRATLAEL TGLGSEARYH AVDVLDAEAL
2221 RTTVKRVHDE HGRLDAVVHA AGVIEDKLVA DKSVESFRRV FGTKVDGART LLDAVDTLPE
2281 PPRHAVLFGS IAAALGNRGQ CDYAAANDAL EELARRWSKP GRRGLTVHWG PWAAAGGGMV
2341 TPELMRSYAA RGIALIDPDE GPLSLLRELA YGADDRHAVV YTASGW
//