LOCUS ACU36265.1 760 aa PRT BCT 24-DEC-2013 DEFINITION Actinosynnema mirum DSM 43827 CheA signal transduction histidine kinase protein. ACCESSION CP001630-2291 PROTEIN_ID ACU36265.1 SOURCE Actinosynnema mirum DSM 43827 ORGANISM Actinosynnema mirum DSM 43827 Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae; Actinosynnema. REFERENCE 1 (bases 1 to 8248144) AUTHORS Land,M., Lapidus,A., Mayilraj,S., Chen,F., Copeland,A., Del Rio,T.G., Nolan,M., Lucas,S., Tice,H., Cheng,J.F., Chertkov,O., Bruce,D., Goodwin,L., Pitluck,S., Rohde,M., Goker,M., Pati,A., Ivanova,N., Mavromatis,K., Chen,A., Palaniappan,K., Hauser,L., Chang,Y.J., Jeffries,C.C., Brettin,T., Detter,J.C., Han,C., Chain,P., Tindall,B.J., Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C. and Klenk,H.P. TITLE Complete genome sequence of Actinosynnema mirum type strain (101) JOURNAL Stand Genomic Sci 1 (1), 46-53 (2009) PUBMED 21304636 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 8248144) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE The complete genome of Actinosynnema mirum DSM 43827 JOURNAL Unpublished REFERENCE 3 (bases 1 to 8248144) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE Direct Submission JOURNAL Submitted (29-MAY-2009) US DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4082379 Source DNA and organism available from Hans-Peter Klenk at the German Collection of Microorganisms and Cell Cultures (DSMZ) (hans-peter.klenk@dsmz.de) Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu) David Bruce (microbe@cuba.jgi-psf.org) Whole genome sequencing and draft assembly at JGI-PGF Finishing done by JGI-LANL Annotation by JGI-ORNL and JGI-PGF The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##Metadata-START## Organism Display Name :: Actinosynnema mirum 101, DSM 43827 Culture Collection ID :: DSM 43827, ATCC 29888, NBRC 14064 GOLD Stamp ID :: Gc01024 Funding Program :: DOE-GEBA 2007 Assembly Method :: Newbler version 1.1.02.15, Phrap Sequencing Depth :: 8.9x Sanger; 20x pyrosequence Gene Calling Method :: Prodigal Isolation Site :: "Blade of grass from Raritan River NJ, USA" Collection Date :: September 1976 Isolation Country :: USA Latitude :: 40.491816 Longitude :: -74.322087 Oxygen Requirement :: Aerobe Motility :: Nonmotile Sporulation :: Sporulating Temperature Range :: Mesophile Temperature Optimum :: 10-30C Gram Staining :: gram+ Biotic Relationship :: Free living Diseases :: None Habitat :: Soil Phenotypes :: Nocardicin A producer Energy Source :: Chemoorganotroph ##Metadata-END## FEATURES Qualifiers source /organism="Actinosynnema mirum DSM 43827" /mol_type="genomic DNA" /strain="DSM 43827" /type_material="type strain of Actinosynnema mirum" /db_xref="taxon:446462" protein /locus_tag="Amir_2325" /inference="protein motif:PFAM:PF01584" /note="PFAM: CheW domain protein; Signal transducing histidine kinase homodimeric; Hpt domain protein; ATP- binding region ATPase domain protein; SMART: CheW domain protein; ATP-binding region ATPase domain protein; Hpt domain protein; KEGG: aba:Acid345_1524 CheA signal transduction histidine kinases" /transl_table=11 /db_xref="InterPro:IPR002545" /db_xref="InterPro:IPR003594" /db_xref="InterPro:IPR004105" /db_xref="InterPro:IPR004358" /db_xref="InterPro:IPR005467" /db_xref="InterPro:IPR008207" BEGIN 1 MDDFDDVVQE FLVESYENLD QLDRDLVALE KEPKSHERLA SIFRTIHTIK GTSGFLAFTK 61 LEQVTHVGES LLVRLRDGTQ ELDKPSADVL LRMVDTVREL LAQIEQNGAE GDLDVQDVID 121 AVTACITGGP AAAPAAPAAA PEVAAVVEAP ATPEASDEEP EGEDAEHDEH DEPEHREEPS 181 APAAVNAPAP LVEAADEGGS KRSVADSSIR VDVNLLDALM RLVGELVLTR NQMIRAADAA 241 GEPNLSRAAQ RLNLITSELQ ESVMKTRMQP IKQLWSKLPR VVRDLSSACG REVELVMEGA 301 ETELDRSLLE AVKDPLTHLV RNAVDHGIEP PETRVANGKS REGTLTLRAY HEHGQVIVEV 361 CDDGAGIHPD KVGATAVKRG VITQSQLESM DNDEILKLVF RPGFSTAAKV SNVSGRGVGM 421 DVVRANIEAI GGAVDLKSVP GKGTTWRLNI PLTLAIVQAL IVDSGDQRYA IPQMAVHELV 481 YVDGQSGSVV EYVSGAPVYR LRGKLLPLVR LSEVLGGPST GKGSDSEIYV AVLKAEGRQF 541 GLVVDRVLNT EEVVVKPLAS RFKDVGCYSG ATILGEGQVS LILDVQSLAR RAHLSSLERT 601 VGAGQDEQVT ASSNGDRLLI TKVADRRIAI PLSMVTRLED FPAKNVERVG TREVVQYRDQ 661 ILPITRLSQL LGAWSETDRE VISAIVYSEG GHSVALAVDS ILDIVESDDV TRSNLDDDGL 721 AGSAVVQQQV TELLDVRRAI LAADPQFFNA DYAGFDMAGA //