LOCUS ACU35986.1 1222 aa PRT BCT 24-DEC-2013
DEFINITION Actinosynnema mirum DSM 43827 nitrate reductase, alpha
subunit protein.
ACCESSION CP001630-2012
PROTEIN_ID ACU35986.1
SOURCE Actinosynnema mirum DSM 43827
ORGANISM Actinosynnema mirum DSM 43827
Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
Actinosynnema.
REFERENCE 1 (bases 1 to 8248144)
AUTHORS Land,M., Lapidus,A., Mayilraj,S., Chen,F., Copeland,A., Del
Rio,T.G., Nolan,M., Lucas,S., Tice,H., Cheng,J.F., Chertkov,O.,
Bruce,D., Goodwin,L., Pitluck,S., Rohde,M., Goker,M., Pati,A.,
Ivanova,N., Mavromatis,K., Chen,A., Palaniappan,K., Hauser,L.,
Chang,Y.J., Jeffries,C.C., Brettin,T., Detter,J.C., Han,C.,
Chain,P., Tindall,B.J., Bristow,J., Eisen,J.A., Markowitz,V.,
Hugenholtz,P., Kyrpides,N.C. and Klenk,H.P.
TITLE Complete genome sequence of Actinosynnema mirum type strain (101)
JOURNAL Stand Genomic Sci 1 (1), 46-53 (2009)
PUBMED 21304636
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 8248144)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N.,
Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C.,
Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F.,
Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE The complete genome of Actinosynnema mirum DSM 43827
JOURNAL Unpublished
REFERENCE 3 (bases 1 to 8248144)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N.,
Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C.,
Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F.,
Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A.
CONSRTM US DOE Joint Genome Institute (JGI-PGF)
TITLE Direct Submission
JOURNAL Submitted (29-MAY-2009) US DOE Joint Genome Institute, 2800
Mitchell Drive, Walnut Creek, CA 94598-1698, USA
COMMENT URL -- http://www.jgi.doe.gov
JGI Project ID: 4082379
Source DNA and organism available from Hans-Peter Klenk at the
German Collection of Microorganisms and Cell Cultures (DSMZ)
(hans-peter.klenk@dsmz.de)
Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu)
David Bruce (microbe@cuba.jgi-psf.org)
Whole genome sequencing and draft assembly at JGI-PGF
Finishing done by JGI-LANL
Annotation by JGI-ORNL and JGI-PGF
The JGI and collaborators endorse the principles for the
distribution and use of large scale sequencing data adopted by the
larger genome sequencing community and urge users of this data to
follow them. It is our intention to publish the work of this
project in a timely fashion and we welcome collaborative
interaction on the project and analysis.
(http://www.genome.gov/page.cfm?pageID=10506376).
##Metadata-START##
Organism Display Name :: Actinosynnema mirum 101, DSM 43827
Culture Collection ID :: DSM 43827, ATCC 29888, NBRC 14064
GOLD Stamp ID :: Gc01024
Funding Program :: DOE-GEBA 2007
Assembly Method :: Newbler version 1.1.02.15, Phrap
Sequencing Depth :: 8.9x Sanger; 20x pyrosequence
Gene Calling Method :: Prodigal
Isolation Site :: "Blade of grass from Raritan River NJ,
USA"
Collection Date :: September 1976
Isolation Country :: USA
Latitude :: 40.491816
Longitude :: -74.322087
Oxygen Requirement :: Aerobe
Motility :: Nonmotile
Sporulation :: Sporulating
Temperature Range :: Mesophile
Temperature Optimum :: 10-30C
Gram Staining :: gram+
Biotic Relationship :: Free living
Diseases :: None
Habitat :: Soil
Phenotypes :: Nocardicin A producer
Energy Source :: Chemoorganotroph
##Metadata-END##
FEATURES Qualifiers
source /organism="Actinosynnema mirum DSM 43827"
/mol_type="genomic DNA"
/strain="DSM 43827"
/type_material="type strain of Actinosynnema mirum"
/db_xref="taxon:446462"
protein /locus_tag="Amir_2040"
/EC_number="1.7.99.4"
/inference="protein motif:TFAM:TIGR01580"
/note="KEGG: bcb:BCB4264_A2135 respiratory nitrate
reductase, alpha subunit; TIGRFAM: nitrate reductase,
alpha subunit; PFAM: molybdopterin oxidoreductase;
molybdopterin oxidoreductase Fe4S4 region; molydopterin
dinucleotide- binding region"
/transl_table=11
/db_xref="InterPro:IPR006468"
/db_xref="InterPro:IPR006655"
/db_xref="InterPro:IPR006656"
/db_xref="InterPro:IPR006657"
/db_xref="InterPro:IPR006963"
BEGIN
1 MDSTGVDRPR PRGRKFFARG EVSADGRTVH RIDQAEWERF YRDRWAHDKV VRSTHGVNCT
61 GSCSWQVFVK DGLITWEHQA TDYPSVGPDS PEYEPRGCPR GASFSWYTYS PSRVRYPYVR
121 GVLLKMWREA KARLGDPVLA WAEITGDPEK SRRYKRARGK GGLIRSTWDE VNELVAAAHV
181 HTAKAYGPDR VVGFSPIPAM SMASFAAGTR YHSLIGGTLL SFYDWYADLP IASPQIWGDQ
241 TDVPESADWW NSGYLVMWGS NIPVTRTPDA HFLTEARYKG TKVVAVSPDY ADNVKFADDW
301 LAPHPGTDGA LAMAMGHVVL TEFFRDRQVP YFSEYTRRYT DLPFLVRLEE RGDAFVPGKF
361 LTAADLGQDV ENAEFKTVLL NEETGEAVVP QGSLGHRWGE QGEGRWNLEL DGVTPALTLH
421 GDNAALTTEA VAIDLPRFDV GETEGGGIVR RGVPATRVNG MLVTTVFDLV MARYAVGREG
481 LPGEWPKSYE DTEAPGTPGW AEEITSVPAA TTARIAREFA RNAERTQGRS MIVVGAGANH
541 WFHSDTIYRA FLSLVLLTGC QGRNGGGWAH YVGQEKARPV TGMQHLAFAF DWQRPTRHMA
601 GTAFWYLATD QWRYEGFKAD ELSTPLGTGM LQGKSFADCN AQAARLGWLP SHPTFDRNPL
661 DLADEAKDAG VPVADHVVSE LKAGRLNFAA EDPDNPANFP RALTVWRANL LGSSGKGNEY
721 FLRHLLGADA QVRGAETAEG NRPQDVTWHD EAPEGKLDLL TAVDFRMTST GLFADVVLPA
781 ATWYEKHDLS STDMHPFVHA FNPAIAPPWE ARSDYDVFLG LADHYSELAK THLGKRTDVL
841 AVPLAHDTPD EMAQPGGRVL DWRNGDCEPI PGRTMPKLVV IERDFAATAD KMRSVGPLLE
901 KLGLTTKGIT YHPEPEIELL RRTNGVRGDN RPSLATAEHM CEAILALSAT TNGRLAVEGF
961 KQLEKRTGTK LADLAEENEG KRISYQEAKK GPQAVLTSPE WSGSETGGRR YSAFVINVER
1021 DKPWHTLTGR QQFFVDHDWL TELGEQLPVY RPPLNMHRRF GSQQLGEPGR AEVTVRYLTP
1081 HSKWSIHSEY QDNAHMLALS RGGPDIWMST QDAERIGVAD NDWVEAYNRN GVVVARAVVS
1141 HRMPEGTVYV YHAKDRNVNV PKTEIGGKRG GVHNSLTRLL LKPTHLVGGY AQFTYAFNYY
1201 GPTGNQRDEV TVVRRRSQEV EY
//