LOCUS ACU35757.1 784 aa PRT BCT 24-DEC-2013 DEFINITION Actinosynnema mirum DSM 43827 Kojibiose phosphorylase protein. ACCESSION CP001630-1783 PROTEIN_ID ACU35757.1 SOURCE Actinosynnema mirum DSM 43827 ORGANISM Actinosynnema mirum DSM 43827 Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae; Actinosynnema. REFERENCE 1 (bases 1 to 8248144) AUTHORS Land,M., Lapidus,A., Mayilraj,S., Chen,F., Copeland,A., Del Rio,T.G., Nolan,M., Lucas,S., Tice,H., Cheng,J.F., Chertkov,O., Bruce,D., Goodwin,L., Pitluck,S., Rohde,M., Goker,M., Pati,A., Ivanova,N., Mavromatis,K., Chen,A., Palaniappan,K., Hauser,L., Chang,Y.J., Jeffries,C.C., Brettin,T., Detter,J.C., Han,C., Chain,P., Tindall,B.J., Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C. and Klenk,H.P. TITLE Complete genome sequence of Actinosynnema mirum type strain (101) JOURNAL Stand Genomic Sci 1 (1), 46-53 (2009) PUBMED 21304636 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 8248144) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE The complete genome of Actinosynnema mirum DSM 43827 JOURNAL Unpublished REFERENCE 3 (bases 1 to 8248144) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE Direct Submission JOURNAL Submitted (29-MAY-2009) US DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4082379 Source DNA and organism available from Hans-Peter Klenk at the German Collection of Microorganisms and Cell Cultures (DSMZ) (hans-peter.klenk@dsmz.de) Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu) David Bruce (microbe@cuba.jgi-psf.org) Whole genome sequencing and draft assembly at JGI-PGF Finishing done by JGI-LANL Annotation by JGI-ORNL and JGI-PGF The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##Metadata-START## Organism Display Name :: Actinosynnema mirum 101, DSM 43827 Culture Collection ID :: DSM 43827, ATCC 29888, NBRC 14064 GOLD Stamp ID :: Gc01024 Funding Program :: DOE-GEBA 2007 Assembly Method :: Newbler version 1.1.02.15, Phrap Sequencing Depth :: 8.9x Sanger; 20x pyrosequence Gene Calling Method :: Prodigal Isolation Site :: "Blade of grass from Raritan River NJ, USA" Collection Date :: September 1976 Isolation Country :: USA Latitude :: 40.491816 Longitude :: -74.322087 Oxygen Requirement :: Aerobe Motility :: Nonmotile Sporulation :: Sporulating Temperature Range :: Mesophile Temperature Optimum :: 10-30C Gram Staining :: gram+ Biotic Relationship :: Free living Diseases :: None Habitat :: Soil Phenotypes :: Nocardicin A producer Energy Source :: Chemoorganotroph ##Metadata-END## FEATURES Qualifiers source /organism="Actinosynnema mirum DSM 43827" /mol_type="genomic DNA" /strain="DSM 43827" /type_material="type strain of Actinosynnema mirum" /db_xref="taxon:446462" protein /locus_tag="Amir_1809" /EC_number="2.4.1.230" /inference="protein motif:PRIAM:2.4.1.230" /note="PFAM: glycoside hydrolase family 65 central catalytic; glycoside hydrolase family 65 domain protein; KEGG: mex:Mext_0562 kojibiose phosphorylase" /transl_table=11 /db_xref="InterPro:IPR005194" /db_xref="InterPro:IPR005195" /db_xref="InterPro:IPR005196" BEGIN 1 MSGYRVQPWK LRWEGLSLTD LRQTESTFAL ANGHIGMRGT LEEGEPRGLP GTYLNGFYEE 61 HDLPYGESGY GYPEEGQTVV NVTDGKVLRL LVEDEPLDLR YGRTIAHEQE LDFRSGTLRR 121 RTEWESPTGK RVIVRTERLV SLTQRAVAAI RYEVEAAQDV QLVVQSDLLA NEPVEERSGD 181 PRVAKALDSP LVADFAYARG KRAVLAHRTR TSGLRMAAAM DHEVDANGSV RTEVEADEDL 241 ARFTVAVDVA AGEKLTIVKY LGYGWSGQRS VPALRAQVEA ALAGALQTGW DGLLEEQRDF 301 LDEFWNSADV EVDGDPELQQ AVRFALFHVL QAAVRGETRA IAGKGLTGPG YDGHAFWDTE 361 TFVLPVLTYT VPEAARDALA WRHSTVEDAR ERARILGLGG AAFPWRSISG AECSAYWPAS 421 TAAFHVSGDV AYAVAQYAQA TGDEEFERAA GAEILVETAR LWMSLGHHVP GDGFRIDGVT 481 GPDEYTAVVD NNVYTNLMAQ RNLRVAAEVC ERLPDVAQHL GVEDDEPQAW LRAAEAMTLP 541 YDDELGVHQQ SEGFTKHAEW DFEATEADDY PLLLNYPYFD LYRKQVVKQA DLVLAMHLRG 601 DAFTAAQKAR NFAYYEARTV RDSSLSAATQ AVLAAEVGAL ELAYDYLSEA AFTDLHDLHH 661 NVHNGLHMAS LAGVWLGLVA GFGGMRDHSG GLEFAPRLPV GLDRFSFRMT YRGSGLCVVV 721 EEGKTTYRIV SGPGLRIRHH GEEVEVEPEN PLVLQVPAAP EPPEGITQPA GRAPRRRTPG 781 TAQD //