LOCUS ACU35375.1 623 aa PRT BCT 24-DEC-2013 DEFINITION Actinosynnema mirum DSM 43827 6-phosphogluconate dehydratase protein. ACCESSION CP001630-1401 PROTEIN_ID ACU35375.1 SOURCE Actinosynnema mirum DSM 43827 ORGANISM Actinosynnema mirum DSM 43827 Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae; Actinosynnema. REFERENCE 1 (bases 1 to 8248144) AUTHORS Land,M., Lapidus,A., Mayilraj,S., Chen,F., Copeland,A., Del Rio,T.G., Nolan,M., Lucas,S., Tice,H., Cheng,J.F., Chertkov,O., Bruce,D., Goodwin,L., Pitluck,S., Rohde,M., Goker,M., Pati,A., Ivanova,N., Mavromatis,K., Chen,A., Palaniappan,K., Hauser,L., Chang,Y.J., Jeffries,C.C., Brettin,T., Detter,J.C., Han,C., Chain,P., Tindall,B.J., Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C. and Klenk,H.P. TITLE Complete genome sequence of Actinosynnema mirum type strain (101) JOURNAL Stand Genomic Sci 1 (1), 46-53 (2009) PUBMED 21304636 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 8248144) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE The complete genome of Actinosynnema mirum DSM 43827 JOURNAL Unpublished REFERENCE 3 (bases 1 to 8248144) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE Direct Submission JOURNAL Submitted (29-MAY-2009) US DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4082379 Source DNA and organism available from Hans-Peter Klenk at the German Collection of Microorganisms and Cell Cultures (DSMZ) (hans-peter.klenk@dsmz.de) Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu) David Bruce (microbe@cuba.jgi-psf.org) Whole genome sequencing and draft assembly at JGI-PGF Finishing done by JGI-LANL Annotation by JGI-ORNL and JGI-PGF The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##Metadata-START## Organism Display Name :: Actinosynnema mirum 101, DSM 43827 Culture Collection ID :: DSM 43827, ATCC 29888, NBRC 14064 GOLD Stamp ID :: Gc01024 Funding Program :: DOE-GEBA 2007 Assembly Method :: Newbler version 1.1.02.15, Phrap Sequencing Depth :: 8.9x Sanger; 20x pyrosequence Gene Calling Method :: Prodigal Isolation Site :: "Blade of grass from Raritan River NJ, USA" Collection Date :: September 1976 Isolation Country :: USA Latitude :: 40.491816 Longitude :: -74.322087 Oxygen Requirement :: Aerobe Motility :: Nonmotile Sporulation :: Sporulating Temperature Range :: Mesophile Temperature Optimum :: 10-30C Gram Staining :: gram+ Biotic Relationship :: Free living Diseases :: None Habitat :: Soil Phenotypes :: Nocardicin A producer Energy Source :: Chemoorganotroph ##Metadata-END## FEATURES Qualifiers source /organism="Actinosynnema mirum DSM 43827" /mol_type="genomic DNA" /strain="DSM 43827" /type_material="type strain of Actinosynnema mirum" /db_xref="taxon:446462" protein /locus_tag="Amir_1423" /EC_number="4.2.1.12" /inference="protein motif:TFAM:TIGR01196" /note="KEGG: pap:PSPA7_1934 phosphogluconate dehydratase; TIGRFAM: 6-phosphogluconate dehydratase; PFAM: dihydroxy-acid and 6-phosphogluconate dehydratase" /transl_table=11 /db_xref="InterPro:IPR000581" /db_xref="InterPro:IPR004786" BEGIN 1 MSDLHPVVAE VTARIAARSA ASRSAYLERV NAAALEGRPA RGDLACSNLA HGFAGITGGD 61 KQALRAVRKP NVAIVSAYND MLSAHQPMDE YPSWIKEAAR GAGGIAQFAG GVPAMCDGVT 121 QGRAGMELSL YSRDVIAMST AIALSHDMFD STLLLGVCDK IVPGLLIGAL SFGHLPAILV 181 PAGPMESGLP NKEKARVRQL FAEGKATRED LLEAEAASYH SPGTCTFYGT ANSNQLVVEV 241 MGLHLPGATF VPPSTPLRKA LTEEAARRAV ALSRGEEFTP LGHVVDVKSV VNGVIALLAT 301 GGSTNHTMHL VAIASAAGVE LGWDDFAELS SVVPLLARVY PNGGADINHF QAAGGVQFLV 361 GTLLDAGLLH GDVRTVAGPG LDRYRREPVL VDGELVWRDG PTETLDAAVL RPASDPFSAD 421 GGLRMLSGNL GRAVIKVSAV AEEHRVVEAP ARVFTSQEAF GTAFKAGELD RDVVVVVRQQ 481 GPQANGMPEL HKLTPALGVL MDRGFKVALV TDGRMSGASG KIPAAIQVTP EAAVGGPLAR 541 VRDGDVLRVD AGACSLDVVV DPVELGAREL VDFPPDAQSW TGTGRELFAA LRRSVGPADR 601 GASVFGPIAA SHFVGLSNQE VSR //