LOCUS ACU34910.1 772 aa PRT BCT 24-DEC-2013 DEFINITION Actinosynnema mirum DSM 43827 ATP-dependent helicase HrpB protein. ACCESSION CP001630-936 PROTEIN_ID ACU34910.1 SOURCE Actinosynnema mirum DSM 43827 ORGANISM Actinosynnema mirum DSM 43827 Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae; Actinosynnema. REFERENCE 1 (bases 1 to 8248144) AUTHORS Land,M., Lapidus,A., Mayilraj,S., Chen,F., Copeland,A., Del Rio,T.G., Nolan,M., Lucas,S., Tice,H., Cheng,J.F., Chertkov,O., Bruce,D., Goodwin,L., Pitluck,S., Rohde,M., Goker,M., Pati,A., Ivanova,N., Mavromatis,K., Chen,A., Palaniappan,K., Hauser,L., Chang,Y.J., Jeffries,C.C., Brettin,T., Detter,J.C., Han,C., Chain,P., Tindall,B.J., Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C. and Klenk,H.P. TITLE Complete genome sequence of Actinosynnema mirum type strain (101) JOURNAL Stand Genomic Sci 1 (1), 46-53 (2009) PUBMED 21304636 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 8248144) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE The complete genome of Actinosynnema mirum DSM 43827 JOURNAL Unpublished REFERENCE 3 (bases 1 to 8248144) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Klenk,H.-P. and Eisen,J.A. CONSRTM US DOE Joint Genome Institute (JGI-PGF) TITLE Direct Submission JOURNAL Submitted (29-MAY-2009) US DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4082379 Source DNA and organism available from Hans-Peter Klenk at the German Collection of Microorganisms and Cell Cultures (DSMZ) (hans-peter.klenk@dsmz.de) Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu) David Bruce (microbe@cuba.jgi-psf.org) Whole genome sequencing and draft assembly at JGI-PGF Finishing done by JGI-LANL Annotation by JGI-ORNL and JGI-PGF The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). ##Metadata-START## Organism Display Name :: Actinosynnema mirum 101, DSM 43827 Culture Collection ID :: DSM 43827, ATCC 29888, NBRC 14064 GOLD Stamp ID :: Gc01024 Funding Program :: DOE-GEBA 2007 Assembly Method :: Newbler version 1.1.02.15, Phrap Sequencing Depth :: 8.9x Sanger; 20x pyrosequence Gene Calling Method :: Prodigal Isolation Site :: "Blade of grass from Raritan River NJ, USA" Collection Date :: September 1976 Isolation Country :: USA Latitude :: 40.491816 Longitude :: -74.322087 Oxygen Requirement :: Aerobe Motility :: Nonmotile Sporulation :: Sporulating Temperature Range :: Mesophile Temperature Optimum :: 10-30C Gram Staining :: gram+ Biotic Relationship :: Free living Diseases :: None Habitat :: Soil Phenotypes :: Nocardicin A producer Energy Source :: Chemoorganotroph ##Metadata-END## FEATURES Qualifiers source /organism="Actinosynnema mirum DSM 43827" /mol_type="genomic DNA" /strain="DSM 43827" /type_material="type strain of Actinosynnema mirum" /db_xref="taxon:446462" protein /locus_tag="Amir_0951" /inference="protein motif:TFAM:TIGR01970" /note="KEGG: rce:RC1_1438 ATP-dependent helicase protein, putative; TIGRFAM: ATP-dependent helicase HrpB; PFAM: Helicase ATP-dependent domain protein; helicase domain protein; helicase-associated domain protein; DEAD/DEAH box helicase domain protein; SMART: DEAD-like helicase; helicase domain protein" /transl_table=11 /db_xref="InterPro:IPR001650" /db_xref="InterPro:IPR007502" /db_xref="InterPro:IPR010225" /db_xref="InterPro:IPR011545" /db_xref="InterPro:IPR013689" /db_xref="InterPro:IPR014001" /db_xref="InterPro:IPR014021" BEGIN 1 MELPDLPVRA VLPELIETLS GWGAAVLVAP PGTGKTTLVP LALEGRVVIA EPRRIAARAA 61 ASRMAALLGE PVGATVGYSV RGDRKVSRST RIEVVTSGLL VRRLQHDPEL SGVGTVVLDE 121 CHERHLDADL LLALLLDARA GLRPDLRLLA TSATVAADRL AELMGGVPVL TAHARTYPVE 181 LFHVPPQKNE RSEAVVARAI RRAVAETPGD VLVFLPGVPE IRRVAALFQG GDLRVDVLHG 241 RLTAAEQDAV LRVGREQRVL LSTSIAESSL TVPGVRAVVD AGLSRVPRVD HRRGLAGLAT 301 VRVSAAVADQ RAGRAGREAP GRAYRCWPEH EQSTLPRYPE PEIRSADLTS LALELACWGT 361 PDGEGLSWWD APPRGALEAA KAVLGNLGAL RDGRVTERGE RMSALGLHPR LARALLDGGE 421 LAGSRTATEV VAILDEDRGS GEDITGRSAD RREVGRLSKL IPAASGSGDQ ALVVALAHPE 481 RLARRRAPGS PVYLMAGGTA AELPQGSRLA DAEWLAIAVA DREPGRIHAR IRLAARADEQ 541 LAVQAAPALL GEREEIAWDG DVVARRVRHL GAIPLSTTQL TGTAHLQQAL RAGLAAEGLG 601 LLSWGPAAVR TRQRLAFLHE VLGDPWPEVT DAALLPLVDL GNARRKSDLA KLKADHVLQA 661 AMPWPAAAKF DALAPDRLEV PSGSRIAIDY STTPPTLPVK VQEVFGWTET PTVAGIPLVL 721 HLLTPAGRPT AVTGDLKSFW QTGYPQVRAE LRGRYPKHRW PEDPMTAPPA RR //