LOCUS ACM57213.1 1215 aa PRT BCT 01-MAR-2021 DEFINITION Halorubrum lacusprofundi ATCC 49239 peptidase S8 and S53 subtilisin kexin sedolisin protein. ACCESSION CP001365-1607 PROTEIN_ID ACM57213.1 SOURCE Halorubrum lacusprofundi ATCC 49239 ORGANISM Halorubrum lacusprofundi ATCC 49239 Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Haloferacaceae; Halorubrum. REFERENCE 1 (bases 1 to 2735295) AUTHORS Dassarma,S., Capes,M.D., Karan,R. and Dassarma,P. TITLE Amino acid substitutions in cold-adapted proteins from Halorubrum lacusprofundi, an extremely halophilic microbe from antarctica JOURNAL PLoS One 8 (3), e58587 (2013) PUBMED 23536799 REFERENCE 2 (bases 1 to 2735295) AUTHORS Karan,R., Capes,M.D., DasSarma,P. and DasSarma,S. TITLE Cloning, overexpression, purification, and characterization of a polyextremophilic beta-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi JOURNAL BMC Biotechnol 13, 3 (2013) PUBMED 23320757 REMARK Publication Status: Online-Only REFERENCE 3 (bases 1 to 2735295) AUTHORS Anderson,I.J., DasSarma,P., Lucas,S., Copeland,A., Lapidus,A., Del Rio,T.G., Tice,H., Dalin,E., Bruce,D.C., Goodwin,L., Pitluck,S., Sims,D., Brettin,T.S., Detter,J.C., Han,C.S., Larimer,F., Hauser,L., Land,M., Ivanova,N., Richardson,P., Cavicchioli,R., DasSarma,S., Woese,C.R. and Kyrpides,N.C. TITLE Complete genome sequence of the Antarctic Halorubrum lacusprofundi type strain ACAM 34 JOURNAL Stand Genomic Sci 11 (1), 70 (2016) PUBMED 27617060 REMARK Publication Status: Online-Only REFERENCE 4 (bases 1 to 2735295) AUTHORS Laye,V.J., Karan,R., Kim,J.M., Pecher,W.T., DasSarma,P. and DasSarma,S. TITLE Key amino acid residues conferring enhanced enzyme activity at cold temperatures in an Antarctic polyextremophilic beta-galactosidase JOURNAL Proc Natl Acad Sci U S A 114 (47), 12530-12535 (2017) PUBMED 29109294 REFERENCE 5 (bases 1 to 2735295) AUTHORS Laye,V.J. and DasSarma,S. TITLE An Antarctic Extreme Halophile and Its Polyextremophilic Enzyme: Effects of Perchlorate Salts JOURNAL Astrobiology 18 (4), 412-418 (2018) PUBMED 29189043 REFERENCE 6 (bases 1 to 2735295) AUTHORS DasSarma,S., DasSarma,P., Laye,V.J. and Schwieterman,E.W. TITLE Extremophilic models for astrobiology: haloarchaeal survival strategies and pigments for remote sensing JOURNAL Extremophiles 24 (1), 31-41 (2020) PUBMED 31463573 REFERENCE 7 (bases 1 to 2735295) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Anderson,I., DasSarma,S., Cavicchioli,R. and Richardson,P. CONSRTM US DOE Joint Genome Institute TITLE Direct Submission JOURNAL Submitted (14-JAN-2009) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4001421 Source DNA available from Professor Shiladitya DasSarma Archaea available from ATCC: ATCC 49239 Contacts: Ricardo Cavicchioli (r.cavicchioli@unsw.edu.au) Shiladitya DasSarma (sdassarma@som.umaryland.edu) David Bruce (microbe@cuba.jgi-psf.org) Annotation done by JGI-ORNL and JGI-PGF Finishing done by JGI-LANL Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of at least two independent clones. Each base pair has a minimum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. it is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). FEATURES Qualifiers source /organism="Halorubrum lacusprofundi ATCC 49239" /mol_type="genomic DNA" /strain="ATCC 49239" /culture_collection="ATCC:49239" /type_material="type strain of Halorubrum lacusprofundi" /db_xref="taxon:416348" /chromosome="1" protein /locus_tag="Hlac_1628" /inference="protein motif:PFAM:PF00082" /note="PFAM: peptidase S8 and S53 subtilisin kexin sedolisin; KEGG: nph:NP2060A hypothetical protein" /transl_table=11 /db_xref="InterPro:IPR000209" BEGIN 1 MIPSSPFSTA TAATLVLVAC LIVAAVAPGA VAGGLVTSST EAVDSGRTDP AAVGSVSTAD 61 GDVIASDLRS ANGTVKLVVR FAGGTRIGTD DGEVGYSKGS STLSTNDLKT NAASAQADFE 121 SFAEGRSAVT VERSFWLANA MLVTVDTDRV PLDRLVDVPG VEGVHENFEV ELDSATTTTP 181 GDGGSQAIGT SGLPPAPTPE DVSTASTDTD ATYGVEMVRA PEVWETFGTR GKGATVAVID 241 TGIDPDHPDL TVSGWAEYDA DGNLVSDDVS DASDGDGHGT HVAGTVAGGN ASGTAIGVAP 301 NASLHGIKVF DDDGTNATFV RVVAGMEHAT QDPDVDVLQM SLGADGHLPY FIEPVRNTRS 361 AGKIAVVSAG NIGQGTSSSP GNVYDSLAVG AVNDSRGVAD FSSGETINTS SAWGSDAPAD 421 WPDEYVVPDV SAPGVSVYSA EPGGTYIRKD GTSMAAPHVS GVAALMLSAS SGDVSDDKLY 481 DTLRNTANHP SNAADPDDRY GAGIVDGFEA VSSVTSDEFT VTEFDGPKET DPGATVEASA 541 TVTNTGGGPG TGTVEYQFNG TVGNDTTVSL DPGEATTVSF TYVVPHETDP DEYSHGVYTE 601 DSSQASTITV REPPSYTVTD LTTPETVERG GPLTTTVNVT NDGEVAGDNR TVELRLVDPG 661 NASNASALGA ENVSLGPGNT TTVSLSGTVP SGFGTGETTV TVVSPEDDAS ASIRIAEAVG 721 TISGTVTDAE TNASLAEIDV VVKNGTEVVG TTITGSEGTY AIDVPATDLT VIASNATYAP 781 ADQTVALAGS GDTATANISL ALRNGTLSGV VGASDGLDLP SNATVTVTDE TGGAVAAVDA 841 ASDGTYSVDL RPGTYNATAD APDFDTQTVA DIAVSPNATA SESFALIPQR GSLSGMVTNA 901 TDSEAIPDAT ITVDGERTAT TDANGTYALD ALERGEREIT VSADGYAQKS QTLTFTANDT 961 RELNVSLSPR GAFVITQLSG DDEIEQGSSG GFGLTVRNDG RVTDDASVDV TLNRSGSVSP 1021 NPVVIGDVAV GDTESESVSV SLGSSAATGT YAVTATTPDS EKTLTFVAVS SDDAESDGGG 1081 TAGGGGGTAG AGPAPPPSTE DPDEPENATD DPTNETDDPT NATDDPTNAT DDPTDGEDDP 1141 VDGELADGDD ENNAEDDGID TESGVATDGE SGGDDTSDED ESADGTPGFG PAIGVLAIFG 1201 AAFLVGRRGG RGGRE //