LOCUS ACM56110.1 903 aa PRT BCT 01-MAR-2021
DEFINITION Halorubrum lacusprofundi ATCC 49239 pyruvate phosphate
dikinase PEP/pyruvate-binding protein.
ACCESSION CP001365-504
PROTEIN_ID ACM56110.1
SOURCE Halorubrum lacusprofundi ATCC 49239
ORGANISM Halorubrum lacusprofundi ATCC 49239
Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria;
Haloferacales; Haloferacaceae; Halorubrum.
REFERENCE 1 (bases 1 to 2735295)
AUTHORS Dassarma,S., Capes,M.D., Karan,R. and Dassarma,P.
TITLE Amino acid substitutions in cold-adapted proteins from Halorubrum
lacusprofundi, an extremely halophilic microbe from antarctica
JOURNAL PLoS One 8 (3), e58587 (2013)
PUBMED 23536799
REFERENCE 2 (bases 1 to 2735295)
AUTHORS Karan,R., Capes,M.D., DasSarma,P. and DasSarma,S.
TITLE Cloning, overexpression, purification, and characterization of a
polyextremophilic beta-galactosidase from the Antarctic
haloarchaeon Halorubrum lacusprofundi
JOURNAL BMC Biotechnol 13, 3 (2013)
PUBMED 23320757
REMARK Publication Status: Online-Only
REFERENCE 3 (bases 1 to 2735295)
AUTHORS Anderson,I.J., DasSarma,P., Lucas,S., Copeland,A., Lapidus,A., Del
Rio,T.G., Tice,H., Dalin,E., Bruce,D.C., Goodwin,L., Pitluck,S.,
Sims,D., Brettin,T.S., Detter,J.C., Han,C.S., Larimer,F.,
Hauser,L., Land,M., Ivanova,N., Richardson,P., Cavicchioli,R.,
DasSarma,S., Woese,C.R. and Kyrpides,N.C.
TITLE Complete genome sequence of the Antarctic Halorubrum lacusprofundi
type strain ACAM 34
JOURNAL Stand Genomic Sci 11 (1), 70 (2016)
PUBMED 27617060
REMARK Publication Status: Online-Only
REFERENCE 4 (bases 1 to 2735295)
AUTHORS Laye,V.J., Karan,R., Kim,J.M., Pecher,W.T., DasSarma,P. and
DasSarma,S.
TITLE Key amino acid residues conferring enhanced enzyme activity at cold
temperatures in an Antarctic polyextremophilic beta-galactosidase
JOURNAL Proc Natl Acad Sci U S A 114 (47), 12530-12535 (2017)
PUBMED 29109294
REFERENCE 5 (bases 1 to 2735295)
AUTHORS Laye,V.J. and DasSarma,S.
TITLE An Antarctic Extreme Halophile and Its Polyextremophilic Enzyme:
Effects of Perchlorate Salts
JOURNAL Astrobiology 18 (4), 412-418 (2018)
PUBMED 29189043
REFERENCE 6 (bases 1 to 2735295)
AUTHORS DasSarma,S., DasSarma,P., Laye,V.J. and Schwieterman,E.W.
TITLE Extremophilic models for astrobiology: haloarchaeal survival
strategies and pigments for remote sensing
JOURNAL Extremophiles 24 (1), 31-41 (2020)
PUBMED 31463573
REFERENCE 7 (bases 1 to 2735295)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T.,
Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N.,
Ivanova,N., Anderson,I., DasSarma,S., Cavicchioli,R. and
Richardson,P.
CONSRTM US DOE Joint Genome Institute
TITLE Direct Submission
JOURNAL Submitted (14-JAN-2009) US DOE Joint Genome Institute, 2800
Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA
COMMENT URL -- http://www.jgi.doe.gov
JGI Project ID: 4001421
Source DNA available from Professor Shiladitya DasSarma
Archaea available from ATCC: ATCC 49239
Contacts: Ricardo Cavicchioli (r.cavicchioli@unsw.edu.au)
Shiladitya DasSarma (sdassarma@som.umaryland.edu)
David Bruce (microbe@cuba.jgi-psf.org)
Annotation done by JGI-ORNL and JGI-PGF
Finishing done by JGI-LANL
Finished microbial genomes have been curated to close all gaps with
greater than 98% coverage of at least two independent clones. Each
base pair has a minimum q (quality) value of 30 and the total error
rate is less than one per 50000.
The JGI and collaborators endorse the principles for the
distribution and use of large scale sequencing data adopted by the
larger genome sequencing community and urge users of this data to
follow them. it is our intention to publish the work of this
project in a timely fashion and we welcome collaborative
interaction on the project and analysis.
(http://www.genome.gov/page.cfm?pageID=10506376).
FEATURES Qualifiers
source /organism="Halorubrum lacusprofundi ATCC 49239"
/mol_type="genomic DNA"
/strain="ATCC 49239"
/culture_collection="ATCC:49239"
/type_material="type strain of Halorubrum lacusprofundi"
/db_xref="taxon:416348"
/chromosome="1"
protein /locus_tag="Hlac_0508"
/inference="protein motif:PFAM:PF01326"
/note="PFAM: pyruvate phosphate dikinase
PEP/pyruvate-binding; PEP-utilising protein mobile region;
KEGG: rci:RCIX1188 phosphoenolpyruvate synthase"
/transl_table=11
/db_xref="InterPro:IPR002192"
/db_xref="InterPro:IPR008279"
BEGIN
1 MTQNPQPHYV RRFDSLGADD LGVAGGKGVN LGVLVDAGLP VPSGFVVTTA AYRTVTDDAE
61 IREAIKQLDS HDSRDSGALA TTATEIRSLI RDRPVGEPIT RAIADALDGD ASTTYAVRSS
121 ATAEDLATAS FAGQHDTHLG VTADAIVDRV RGCMASLFTD RAVAYRARNG ISHTEVEMAV
181 VVQEMVDADA AGVLFTADPE TGKRTVATVD ATHGLGDTVV AGEVSADHAR IARETGAVIE
241 YEVGEKATEL RLTQEGTTSR DTQMGRRETR VLTDDQLRAL VDVGERIEAL FGEPQDIEWA
301 LADGEFVVLQ SRPITSLVSL PVPRPDDDRL HVYLSLGHGQ AMTDPMPPLA LDLWETVYGE
361 VMNGFTGSNR VWITRTEGRA YMDITPFLGF RQTREGIVET IAAVSRSAAE GTEQLLAERR
421 GEFDIDMSLA TLPALLRTAA SVLPILARLF WQGIAPFVRG AAGIDEFVTE LNGWAREQAA
481 DILADDEPAE LVENVFEGFS TEFVSEVSPK SMRVVIGPLA GTVIEWVVPD ADATIVEAAA
541 RGSEAEVGTR MTLALGNIAD IARETPAVEQ AILDGRPYEA VCAVDGSEAF VEAFDEFIVE
601 FGHRAVGEFD PSRPRWRDDP SAPLGIVRGN LAGEKPGAHQ SRLRERKREA QAAIDELQAS
661 AKRGLLGPVR GPLVSHLLRT YRSHIHLRDE PKHAVAHLFA AWHEAIQRAG EHLVLEDALD
721 DPNDVWFLRR GELFSLIEDP DSEVPDIAVR KREHERHGRI DIPPLITSEG EIPRVERTDV
781 DEHTLVGTGV SAGVVEGVAR IVVDPAHTTL QSGEILVCPS SDPAWTPLFA TAGGLVTEVG
841 GRLTHGALVA REYGLPAVVS VADATEVISD GQRIRVNGDT GTVELLDEQT SDMTEASKTT
901 ESG
//