LOCUS ACM55864.1 864 aa PRT BCT 01-MAR-2021
DEFINITION Halorubrum lacusprofundi ATCC 49239 DNA topoisomerase
type IA central domain protein protein.
ACCESSION CP001365-258
PROTEIN_ID ACM55864.1
SOURCE Halorubrum lacusprofundi ATCC 49239
ORGANISM Halorubrum lacusprofundi ATCC 49239
Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria;
Haloferacales; Haloferacaceae; Halorubrum.
REFERENCE 1 (bases 1 to 2735295)
AUTHORS Dassarma,S., Capes,M.D., Karan,R. and Dassarma,P.
TITLE Amino acid substitutions in cold-adapted proteins from Halorubrum
lacusprofundi, an extremely halophilic microbe from antarctica
JOURNAL PLoS One 8 (3), e58587 (2013)
PUBMED 23536799
REFERENCE 2 (bases 1 to 2735295)
AUTHORS Karan,R., Capes,M.D., DasSarma,P. and DasSarma,S.
TITLE Cloning, overexpression, purification, and characterization of a
polyextremophilic beta-galactosidase from the Antarctic
haloarchaeon Halorubrum lacusprofundi
JOURNAL BMC Biotechnol 13, 3 (2013)
PUBMED 23320757
REMARK Publication Status: Online-Only
REFERENCE 3 (bases 1 to 2735295)
AUTHORS Anderson,I.J., DasSarma,P., Lucas,S., Copeland,A., Lapidus,A., Del
Rio,T.G., Tice,H., Dalin,E., Bruce,D.C., Goodwin,L., Pitluck,S.,
Sims,D., Brettin,T.S., Detter,J.C., Han,C.S., Larimer,F.,
Hauser,L., Land,M., Ivanova,N., Richardson,P., Cavicchioli,R.,
DasSarma,S., Woese,C.R. and Kyrpides,N.C.
TITLE Complete genome sequence of the Antarctic Halorubrum lacusprofundi
type strain ACAM 34
JOURNAL Stand Genomic Sci 11 (1), 70 (2016)
PUBMED 27617060
REMARK Publication Status: Online-Only
REFERENCE 4 (bases 1 to 2735295)
AUTHORS Laye,V.J., Karan,R., Kim,J.M., Pecher,W.T., DasSarma,P. and
DasSarma,S.
TITLE Key amino acid residues conferring enhanced enzyme activity at cold
temperatures in an Antarctic polyextremophilic beta-galactosidase
JOURNAL Proc Natl Acad Sci U S A 114 (47), 12530-12535 (2017)
PUBMED 29109294
REFERENCE 5 (bases 1 to 2735295)
AUTHORS Laye,V.J. and DasSarma,S.
TITLE An Antarctic Extreme Halophile and Its Polyextremophilic Enzyme:
Effects of Perchlorate Salts
JOURNAL Astrobiology 18 (4), 412-418 (2018)
PUBMED 29189043
REFERENCE 6 (bases 1 to 2735295)
AUTHORS DasSarma,S., DasSarma,P., Laye,V.J. and Schwieterman,E.W.
TITLE Extremophilic models for astrobiology: haloarchaeal survival
strategies and pigments for remote sensing
JOURNAL Extremophiles 24 (1), 31-41 (2020)
PUBMED 31463573
REFERENCE 7 (bases 1 to 2735295)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T.,
Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N.,
Ivanova,N., Anderson,I., DasSarma,S., Cavicchioli,R. and
Richardson,P.
CONSRTM US DOE Joint Genome Institute
TITLE Direct Submission
JOURNAL Submitted (14-JAN-2009) US DOE Joint Genome Institute, 2800
Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA
COMMENT URL -- http://www.jgi.doe.gov
JGI Project ID: 4001421
Source DNA available from Professor Shiladitya DasSarma
Archaea available from ATCC: ATCC 49239
Contacts: Ricardo Cavicchioli (r.cavicchioli@unsw.edu.au)
Shiladitya DasSarma (sdassarma@som.umaryland.edu)
David Bruce (microbe@cuba.jgi-psf.org)
Annotation done by JGI-ORNL and JGI-PGF
Finishing done by JGI-LANL
Finished microbial genomes have been curated to close all gaps with
greater than 98% coverage of at least two independent clones. Each
base pair has a minimum q (quality) value of 30 and the total error
rate is less than one per 50000.
The JGI and collaborators endorse the principles for the
distribution and use of large scale sequencing data adopted by the
larger genome sequencing community and urge users of this data to
follow them. it is our intention to publish the work of this
project in a timely fashion and we welcome collaborative
interaction on the project and analysis.
(http://www.genome.gov/page.cfm?pageID=10506376).
FEATURES Qualifiers
source /organism="Halorubrum lacusprofundi ATCC 49239"
/mol_type="genomic DNA"
/strain="ATCC 49239"
/culture_collection="ATCC:49239"
/type_material="type strain of Halorubrum lacusprofundi"
/db_xref="taxon:416348"
/chromosome="1"
protein /locus_tag="Hlac_0259"
/inference="protein motif:PFAM:PF01131"
/note="PFAM: TOPRIM domain protein; DNA topoisomerase type
IA central domain protein; DNA topoisomerase type IA zn
finger domain protein; SMART: Helix-hairpin-helix
DNA-binding class 1; DNA topoisomerase I ATP-binding; DNA
topoisomerase I DNA-binding; Toprim sub domain protein;
KEGG: hwa:HQ1267A DNA topoisomerase I"
/transl_table=11
/db_xref="InterPro:IPR000380"
/db_xref="InterPro:IPR003583"
/db_xref="InterPro:IPR003601"
/db_xref="InterPro:IPR003602"
/db_xref="InterPro:IPR006154"
/db_xref="InterPro:IPR006171"
/db_xref="InterPro:IPR013497"
/db_xref="InterPro:IPR013498"
BEGIN
1 MSRGPELIIT EKDNAARRIA DILSGESATA ERENDVNVYK WGGKRCIGLS GHVVGVDFPA
61 EYNDWRDVEP VELIDAPITK EPTQEGIVAA LRKLARNASR VVIATDYDRE GELIGKEAYE
121 LVREVNEDAP IDRVRFSSIT DNEVNEAFAN PDELDFDLAA AGEARQVIDL TWGAALTRFL
181 SLSARQLGED FISVGRVQGP TLKLIVDRER EIKAFDPEAY WELYGNLTKS GGDPFEARYF
241 YLNDEGNEAE RVWNGDVAEV LTEAFDAADE AVVDDVRRRT RTDDPPTPFN TTAFIRAAGS
301 LGHSAQRAMS LAEDLYTAGY VTYPRTDNTV YPEDLDPREL IEELSVASTF GKDAKSLLEQ
361 EEIEPTEGDE ETTDHPPIHP TGELPSASDL SEDEWEVYEL IVRRFLATCA EPATWERLRV
421 VALANDEATA IAERADGLAA LRNPEEASGP GDLVADGGLR LKANGKRLLE AGYHDVYPYR
481 SSDERIVPDV EVGETLALTD RRTDAKETQP PRRYGQSRLI EEMEKRGVGT KATRHRTLEK
541 LYDRNYIESD PPRPTRLAEA VVEAAEEFAE HVVSEEMTAQ LERDMQAIAA GEKGYDEVTE
601 ASRELLNRVF DDLTESREAV GDHLQKSLKA DKTLGPCPEC GSDLLVRKSR QGSYFVGCDG
661 YPDCEYTLPL PSSGKPLLLD ETCEEHELRH VKMLAGRKTF VHGCPQCKAD EADEQEDEVI
721 GACPECGEEH GGELAIKRLR SGSRLVGCTR YPDCDYSLPL PRRGEIEVTD EICEEHGLPH
781 LRVHSGDEPW ELGCPICNYR EFTARQEGSE LQTVEGIGEK TAEKLKDAGV DGVDDLKSID
841 PDELAADVDG VGADTVRDWQ AKAD
//