LOCUS ACM55782.1 929 aa PRT BCT 01-MAR-2021 DEFINITION Halorubrum lacusprofundi ATCC 49239 DNA polymerase B region protein. ACCESSION CP001365-176 PROTEIN_ID ACM55782.1 SOURCE Halorubrum lacusprofundi ATCC 49239 ORGANISM Halorubrum lacusprofundi ATCC 49239 Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Haloferacaceae; Halorubrum. REFERENCE 1 (bases 1 to 2735295) AUTHORS Dassarma,S., Capes,M.D., Karan,R. and Dassarma,P. TITLE Amino acid substitutions in cold-adapted proteins from Halorubrum lacusprofundi, an extremely halophilic microbe from antarctica JOURNAL PLoS One 8 (3), e58587 (2013) PUBMED 23536799 REFERENCE 2 (bases 1 to 2735295) AUTHORS Karan,R., Capes,M.D., DasSarma,P. and DasSarma,S. TITLE Cloning, overexpression, purification, and characterization of a polyextremophilic beta-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi JOURNAL BMC Biotechnol 13, 3 (2013) PUBMED 23320757 REMARK Publication Status: Online-Only REFERENCE 3 (bases 1 to 2735295) AUTHORS Anderson,I.J., DasSarma,P., Lucas,S., Copeland,A., Lapidus,A., Del Rio,T.G., Tice,H., Dalin,E., Bruce,D.C., Goodwin,L., Pitluck,S., Sims,D., Brettin,T.S., Detter,J.C., Han,C.S., Larimer,F., Hauser,L., Land,M., Ivanova,N., Richardson,P., Cavicchioli,R., DasSarma,S., Woese,C.R. and Kyrpides,N.C. TITLE Complete genome sequence of the Antarctic Halorubrum lacusprofundi type strain ACAM 34 JOURNAL Stand Genomic Sci 11 (1), 70 (2016) PUBMED 27617060 REMARK Publication Status: Online-Only REFERENCE 4 (bases 1 to 2735295) AUTHORS Laye,V.J., Karan,R., Kim,J.M., Pecher,W.T., DasSarma,P. and DasSarma,S. TITLE Key amino acid residues conferring enhanced enzyme activity at cold temperatures in an Antarctic polyextremophilic beta-galactosidase JOURNAL Proc Natl Acad Sci U S A 114 (47), 12530-12535 (2017) PUBMED 29109294 REFERENCE 5 (bases 1 to 2735295) AUTHORS Laye,V.J. and DasSarma,S. TITLE An Antarctic Extreme Halophile and Its Polyextremophilic Enzyme: Effects of Perchlorate Salts JOURNAL Astrobiology 18 (4), 412-418 (2018) PUBMED 29189043 REFERENCE 6 (bases 1 to 2735295) AUTHORS DasSarma,S., DasSarma,P., Laye,V.J. and Schwieterman,E.W. TITLE Extremophilic models for astrobiology: haloarchaeal survival strategies and pigments for remote sensing JOURNAL Extremophiles 24 (1), 31-41 (2020) PUBMED 31463573 REFERENCE 7 (bases 1 to 2735295) AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Anderson,I., DasSarma,S., Cavicchioli,R. and Richardson,P. CONSRTM US DOE Joint Genome Institute TITLE Direct Submission JOURNAL Submitted (14-JAN-2009) US DOE Joint Genome Institute, 2800 Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA COMMENT URL -- http://www.jgi.doe.gov JGI Project ID: 4001421 Source DNA available from Professor Shiladitya DasSarma Archaea available from ATCC: ATCC 49239 Contacts: Ricardo Cavicchioli (r.cavicchioli@unsw.edu.au) Shiladitya DasSarma (sdassarma@som.umaryland.edu) David Bruce (microbe@cuba.jgi-psf.org) Annotation done by JGI-ORNL and JGI-PGF Finishing done by JGI-LANL Finished microbial genomes have been curated to close all gaps with greater than 98% coverage of at least two independent clones. Each base pair has a minimum q (quality) value of 30 and the total error rate is less than one per 50000. The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. it is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376). FEATURES Qualifiers source /organism="Halorubrum lacusprofundi ATCC 49239" /mol_type="genomic DNA" /strain="ATCC 49239" /culture_collection="ATCC:49239" /type_material="type strain of Halorubrum lacusprofundi" /db_xref="taxon:416348" /chromosome="1" protein /locus_tag="Hlac_0177" /inference="protein motif:PFAM:PF00136" /note="PFAM: DNA polymerase B exonuclease; DNA polymerase B region; SMART: DNA-directed DNA polymerase B; KEGG: hsl:OE1777F DNA-directed DNA polymerase type II" /transl_table=11 /db_xref="InterPro:IPR006133" /db_xref="InterPro:IPR006134" /db_xref="InterPro:IPR006172" BEGIN 1 MTQSGLSDFS SPGDADDGTR DSMETEREDM AAQEAAVVAG GGRGHVSDVV DVDEAKFPES 61 TGTVELMITQ IDYAVEGYGS DEYPVVHVFG RRPVDDGDDA VEHLRVLGVE PYFYVPTADL 121 NGDPTEEYDV VIGTREENVE GEPYESIRGE PLTRIVTRTP RDVGNIRDDF ATTFEADILF 181 PNRLLIDNGL NGGVRVQERR LDDGSDAIQV HEGHLEPAEI DAEMRVNTFD IEVDDRRGFP 241 EDGEEPIICL TSHDSYDDEY VVWLYDAPEA EIPPPEDLPD YEGIVGDGGG EIDFEVRTFE 301 TEAAMLDAFI EYIDDTDPDL LTGWNFEDFD APYVLDRLEV LDDGSQYDLS IDRLSRIGEV 361 WRSGWGGPDI KGRVVFDLLY AYKRTMFTEL ESYRLDAVGE RELGVGKERY TGDIGDLWEQ 421 DPERLLEYSI RDVELCVEID RKQDVIAFWD EVRTFVGCKI EDAPTPGDTV DMYVLHKAFG 481 KFALPTKGQQ ESEEFEGGAV FDPITGVKEM VTVQDLKSLY PMCMVTINAG PETKVDPGKY 541 DGEMYVAPNG THFQKEPDGI MREMVDELLS EREEKKGLRN DHDPGTEPYE QYDRQQGAVK 601 VIMNSLYGVT GWDRFRLYDK EGAAAVTATG REVIDFTEEA ANELNYQVSY GDTDSVMLSL 661 SNMSKEEAIE TSFEIEDHIN ERYDDFARDE LNADVHRFQI EFEKLYRRFF QAGKKKRYAG 721 HIIWKEGKDV DDIDITGFEY KRSDIAQITK EVQQNVIETI VTGDDIDEDL EEIKSYLVDV 781 IARVLGGDMD LDEIGIPGGI GKKLDAYDTP TAQVRGAKYA NLMLGTNFGS GSKPKRLYIE 841 KVHPDFWQRM EDEEGLDPQR DHLYGEFKRD PDVICFEYAD QVPDEFKVDW EKMLDKTLQG 901 PIERVIEALG LSWEEVKTGQ EQTGLGSFM //