LOCUS ACM55755.1 1366 aa PRT BCT 01-MAR-2021
DEFINITION Halorubrum lacusprofundi ATCC 49239 DNA polymerase II,
large subunit DP2 protein.
ACCESSION CP001365-149
PROTEIN_ID ACM55755.1
SOURCE Halorubrum lacusprofundi ATCC 49239
ORGANISM Halorubrum lacusprofundi ATCC 49239
Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria;
Haloferacales; Haloferacaceae; Halorubrum.
REFERENCE 1 (bases 1 to 2735295)
AUTHORS Dassarma,S., Capes,M.D., Karan,R. and Dassarma,P.
TITLE Amino acid substitutions in cold-adapted proteins from Halorubrum
lacusprofundi, an extremely halophilic microbe from antarctica
JOURNAL PLoS One 8 (3), e58587 (2013)
PUBMED 23536799
REFERENCE 2 (bases 1 to 2735295)
AUTHORS Karan,R., Capes,M.D., DasSarma,P. and DasSarma,S.
TITLE Cloning, overexpression, purification, and characterization of a
polyextremophilic beta-galactosidase from the Antarctic
haloarchaeon Halorubrum lacusprofundi
JOURNAL BMC Biotechnol 13, 3 (2013)
PUBMED 23320757
REMARK Publication Status: Online-Only
REFERENCE 3 (bases 1 to 2735295)
AUTHORS Anderson,I.J., DasSarma,P., Lucas,S., Copeland,A., Lapidus,A., Del
Rio,T.G., Tice,H., Dalin,E., Bruce,D.C., Goodwin,L., Pitluck,S.,
Sims,D., Brettin,T.S., Detter,J.C., Han,C.S., Larimer,F.,
Hauser,L., Land,M., Ivanova,N., Richardson,P., Cavicchioli,R.,
DasSarma,S., Woese,C.R. and Kyrpides,N.C.
TITLE Complete genome sequence of the Antarctic Halorubrum lacusprofundi
type strain ACAM 34
JOURNAL Stand Genomic Sci 11 (1), 70 (2016)
PUBMED 27617060
REMARK Publication Status: Online-Only
REFERENCE 4 (bases 1 to 2735295)
AUTHORS Laye,V.J., Karan,R., Kim,J.M., Pecher,W.T., DasSarma,P. and
DasSarma,S.
TITLE Key amino acid residues conferring enhanced enzyme activity at cold
temperatures in an Antarctic polyextremophilic beta-galactosidase
JOURNAL Proc Natl Acad Sci U S A 114 (47), 12530-12535 (2017)
PUBMED 29109294
REFERENCE 5 (bases 1 to 2735295)
AUTHORS Laye,V.J. and DasSarma,S.
TITLE An Antarctic Extreme Halophile and Its Polyextremophilic Enzyme:
Effects of Perchlorate Salts
JOURNAL Astrobiology 18 (4), 412-418 (2018)
PUBMED 29189043
REFERENCE 6 (bases 1 to 2735295)
AUTHORS DasSarma,S., DasSarma,P., Laye,V.J. and Schwieterman,E.W.
TITLE Extremophilic models for astrobiology: haloarchaeal survival
strategies and pigments for remote sensing
JOURNAL Extremophiles 24 (1), 31-41 (2020)
PUBMED 31463573
REFERENCE 7 (bases 1 to 2735295)
AUTHORS Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T.,
Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N.,
Ivanova,N., Anderson,I., DasSarma,S., Cavicchioli,R. and
Richardson,P.
CONSRTM US DOE Joint Genome Institute
TITLE Direct Submission
JOURNAL Submitted (14-JAN-2009) US DOE Joint Genome Institute, 2800
Mitchell Drive B310, Walnut Creek, CA 94598-1698, USA
COMMENT URL -- http://www.jgi.doe.gov
JGI Project ID: 4001421
Source DNA available from Professor Shiladitya DasSarma
Archaea available from ATCC: ATCC 49239
Contacts: Ricardo Cavicchioli (r.cavicchioli@unsw.edu.au)
Shiladitya DasSarma (sdassarma@som.umaryland.edu)
David Bruce (microbe@cuba.jgi-psf.org)
Annotation done by JGI-ORNL and JGI-PGF
Finishing done by JGI-LANL
Finished microbial genomes have been curated to close all gaps with
greater than 98% coverage of at least two independent clones. Each
base pair has a minimum q (quality) value of 30 and the total error
rate is less than one per 50000.
The JGI and collaborators endorse the principles for the
distribution and use of large scale sequencing data adopted by the
larger genome sequencing community and urge users of this data to
follow them. it is our intention to publish the work of this
project in a timely fashion and we welcome collaborative
interaction on the project and analysis.
(http://www.genome.gov/page.cfm?pageID=10506376).
FEATURES Qualifiers
source /organism="Halorubrum lacusprofundi ATCC 49239"
/mol_type="genomic DNA"
/strain="ATCC 49239"
/culture_collection="ATCC:49239"
/type_material="type strain of Halorubrum lacusprofundi"
/db_xref="taxon:416348"
/chromosome="1"
protein /locus_tag="Hlac_0150"
/EC_number="2.7.7.7"
/inference="protein motif:TFAM:TIGR00354"
/note="KEGG: hsl:OE4293R DNA-directed DNA polymerase type
III subunit 2 (intein-containing); TIGRFAM: DNA polymerase
II, large subunit DP2; PFAM: DNA polymerase II large
subunit DP2-like; SMART: Hedgehog/intein hint domain
protein"
/transl_table=11
/db_xref="InterPro:IPR003587"
/db_xref="InterPro:IPR004475"
/db_xref="InterPro:IPR006141"
/db_xref="InterPro:IPR016033"
BEGIN
1 MRPDDERYFA QIEDRLDEAW DVAEAAKEQG HDPKPKIEIP IARDMADRVE NILGIDGVAE
61 RVRELEGEMS REEAALELVT DFVEGTVGDY DSRAGKVEGA VRTAVALLTE GVVAAPIEGI
121 DRVELLENDD GTEFVNVYYA GPIRSAGGTA QALSVLVADY ARSLLDIDEY KPRDVEVERY
181 AEEISLYDKE TGLQYSPKDK ESKFIAQHMP VMLDGEATGD EEVSGFRDLE RVDTNSARGG
241 MCLVAAEGIA LKAPKIQRYT RDLDEVDWPW LQDLIDGTIG KDGGGASDEA PENAGAEGDD
301 DSEDTAGNES DGDDADDADS ETDGPAGPLR PKSSQKFLRD LIAGRPVFTH PSEAGGFRLR
361 YGRARNHGFA TGGVHPATMH LVDDFLAAGT QIKTERPGKA HGVIPVDSIE GPTVRLANGE
421 VRRIDDPEEA KEIRNGVEKV LDLGEYLVNY GEFVENNHPL APASYVYEWW VQEFETAGAD
481 VQAMRDDPHV DLEHPDVDEA LAWAREYDCP LHPEYTYLWH DVSVDAFEAL ADAVAAGDVV
541 EGVLAIERTE TTQHALESLL VQHVATPDAL RIPTWRPLAR SLGIDDGLRK TWSADDLSAA
601 AREWDDGENA VKAINEVAPF EIRERAPTRI GNRMGRPEKS ESRDLSPAVH TLFPINEAGG
661 PQRDVAEAAG TMDDSGHRGR LDLEVSDRVC PDCGEHTYRA QCPDCGVHTD LHYECGDCGT
721 VCEPDEAGRV ECPRCEWEVT AATYQEVDLN DAYRSALETV GERESAFEIL KGVQGLTSRN
781 KTPEPIEKGV LRAKNGVTSF KDGTVRYDMT DLPVTSVRAE ELDITADHLR EIGYETDIDG
841 EPLRHDDQLV ELRVQDIVLS DGAAEHMLKT AGFVDDLLEQ FYGLDRFYEF NERDDLVGEL
901 VFGMAPHTSA ATVGRVVGFT SAAVGYAHPY FHAAKRRNCF HPETEIEYRD DAGWHRETIE
961 KFVEERLNDP QTDDFGTLVN ELDGNVEVPS IDEHGNKSTQ PVTAVSKHLS QDHLVQVETQ
1021 RGRSIRVTPD HTMLRVADGG VQKVAANELE IGDSVPTTTL RHEMSVGATV DVTTDGGIEA
1081 DTVASVDFLE SDIEHTYNLT VAETHTLAAN DLLVAQCDGD EDCVMLLMDG LLNFSKTFLP
1141 DQRGGRMDAP LVMSSRIDPS EIDDEAHNVD IAREYPREFY DATLEMADPE SVEDLIQIGE
1201 DTLGTDEEYH GFGHTHDTTD IAMGPDLSAY KTLGDMMEKM DAQLELARKL RAVDETDVAE
1261 RVIEYHFLPD IIGNLRAFSR QKTRCLDCGE KYRRMPLSGD CRECGGRVNL TVHEGSVSKY
1321 VDTAIEVADR FGCRPYTKQR LKVLDQSLES IFEDDTNKQS GIADFM
//