LOCUS ABV14388.1 523 aa PRT BCT 31-JAN-2014 DEFINITION Citrobacter koseri ATCC BAA-895 hypothetical protein protein. ACCESSION CP000822-3209 PROTEIN_ID ABV14388.1 SOURCE Citrobacter koseri ATCC BAA-895 ORGANISM Citrobacter koseri ATCC BAA-895 Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Citrobacter. REFERENCE 1 (bases 1 to 4720462) AUTHORS McClelland,M., Sanderson,E.K., Porwollik,S., Spieth,J., Clifton,W.S., Latreille,P., Courtney,L., Wang,C., Pepin,K., Bhonagiri,V., Nash,W., Johnson,M., Thiruvilangam,P. and Wilson,R. CONSRTM The Citrobacter koseri Genome Sequencing Project TITLE Direct Submission JOURNAL Submitted (29-AUG-2007) Genetics, Genome Sequencing Center, 4444 Forest Park Parkway, St. Louis, MO 63108, USA COMMENT Citrobacter (diversus) koseri--Citrobacter cells are isolated from water, sewage, soils, and food, as well as from the feces of man and other animals, where they may be normal inhabitants. They can be found in urine, sputum, and other clinical specimens. They can sometimes be opportunistic pathogens particularly in immunocompromised patients in hospitals or in infants (Pepperell et al., Antimicrob Agents Chemother. 2002 Nov;46(11):3555-60. and references therein). The strain of Citrobacter koseri being sequenced, strain CDC 4225-83, was isolated in 1983 in Maryland, where it caused neonatal meningitis. It was provided by Caroline Mohr and Melissa Campbell of CDC. The strain is available from the American Type Culture Collection as ATCC BAA-895 or from the Salmonella Genetic Stock Centre as SGSC4696. The genome was sequenced to 8X coverage, using plasmid and fosmid libraries and was finished to an error rate of less than 1 per 10,000 bases. Automated annotation was performed and manual annotation will continue in the labs of Michael McClelland and Kenneth Sanderson. The National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH) has funded this project. Coding sequences below are predicted using GeneMark v3.3 and Glimmer2 v2.13.Intergenic regions not spanned by GeneMark and Glimmer2 were blasted against NCBI's non-redundant (NR) database and predictions generated based on protein alignments. RNA genes were determined usingtRNAscan-SE 1.23 or Rfam v8.0. This sequence was finished as follows unless otherwise noted: all regions were double stranded, sequenced with an alternate chemistries or covered by high quality data (i.e., phred quality >=30); an attempt was made to resolve all sequencing problems, such as compressions and repeats; all regions were covered by sequence from more than one m13 subclone. FEATURES Qualifiers source /organism="Citrobacter koseri ATCC BAA-895" /mol_type="genomic DNA" /strain="ATCC BAA-895" /db_xref="ATCC:BAA-895" /db_xref="taxon:290338" protein /locus_tag="CKO_03304" /inference="protein motif:HMMPfam:IPR000891" /inference="protein motif:HMMPfam:IPR013709" /inference="protein motif:HMMTigr:IPR005671" /inference="protein motif:ScanRegExp:IPR002034" /inference="similar to AA sequence:INSD:ABI99561.1" /note="KEGG: ecp:ECP_0076 6.8e-268 2-isopropylmalate synthase K01649; COG: COG0119 Isopropylmalate/homocitrate/citramalate synthases; Psort location: Cytoplasmic, score:8.96" /transl_table=11 /db_xref="InterPro:IPR000891" /db_xref="InterPro:IPR002034" /db_xref="InterPro:IPR005671" /db_xref="InterPro:IPR013709" BEGIN 1 MSQQVIIFDT TLRDGEQALQ ASLSVKEKLQ IALALERMGV DVMEVGFPVS SPGDFESVQT 61 IARQVKNSRV CALARCVEKD IDVAAESLKV ADAFRIHTFI ATSPMHIATK LRSTLDEVIE 121 RAIYMVKRAR NYTDDVEFSC EDAGRTPIDD LARVVEAAIN AGAKTINIPD TVGYTMPFEF 181 GGIISGLYER VPNIDKAIIS VHTHDDLGLG VGNALAAVHA GARQVEGAMN GIGERAGNCS 241 LEEVIMAIKV RQDILNVHTR INHQEIWRTS QLVSQICNMP IPANKAIVGS GAFAHSSGIH 301 QDGVLKNREN YEIMTPESIG LNQIQLNLTS RSGRAAVKHR MDEMGYKESE YNLDNLYDAF 361 LKLADKKGQV FDYDLEALAF INKQQEEPEH FRLDYFSVQS GSNDIATASV KLACGDDIKA 421 EAANGNGPVD AIYQAINRIT DYNIELVKYS LSAKGHGKDA LGQVDIVANY NGRRFHGVGL 481 ATDIVESSAK AMVHVLNNIW RAAEVEKELQ RKAQNKENNK ETV //