LOCUS ABV11390.1 515 aa PRT BCT 31-JAN-2014 DEFINITION Citrobacter koseri ATCC BAA-895 hypothetical protein protein. ACCESSION CP000822-211 PROTEIN_ID ABV11390.1 SOURCE Citrobacter koseri ATCC BAA-895 ORGANISM Citrobacter koseri ATCC BAA-895 Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Citrobacter. REFERENCE 1 (bases 1 to 4720462) AUTHORS McClelland,M., Sanderson,E.K., Porwollik,S., Spieth,J., Clifton,W.S., Latreille,P., Courtney,L., Wang,C., Pepin,K., Bhonagiri,V., Nash,W., Johnson,M., Thiruvilangam,P. and Wilson,R. CONSRTM The Citrobacter koseri Genome Sequencing Project TITLE Direct Submission JOURNAL Submitted (29-AUG-2007) Genetics, Genome Sequencing Center, 4444 Forest Park Parkway, St. Louis, MO 63108, USA COMMENT Citrobacter (diversus) koseri--Citrobacter cells are isolated from water, sewage, soils, and food, as well as from the feces of man and other animals, where they may be normal inhabitants. They can be found in urine, sputum, and other clinical specimens. They can sometimes be opportunistic pathogens particularly in immunocompromised patients in hospitals or in infants (Pepperell et al., Antimicrob Agents Chemother. 2002 Nov;46(11):3555-60. and references therein). The strain of Citrobacter koseri being sequenced, strain CDC 4225-83, was isolated in 1983 in Maryland, where it caused neonatal meningitis. It was provided by Caroline Mohr and Melissa Campbell of CDC. The strain is available from the American Type Culture Collection as ATCC BAA-895 or from the Salmonella Genetic Stock Centre as SGSC4696. The genome was sequenced to 8X coverage, using plasmid and fosmid libraries and was finished to an error rate of less than 1 per 10,000 bases. Automated annotation was performed and manual annotation will continue in the labs of Michael McClelland and Kenneth Sanderson. The National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH) has funded this project. Coding sequences below are predicted using GeneMark v3.3 and Glimmer2 v2.13.Intergenic regions not spanned by GeneMark and Glimmer2 were blasted against NCBI's non-redundant (NR) database and predictions generated based on protein alignments. RNA genes were determined usingtRNAscan-SE 1.23 or Rfam v8.0. This sequence was finished as follows unless otherwise noted: all regions were double stranded, sequenced with an alternate chemistries or covered by high quality data (i.e., phred quality >=30); an attempt was made to resolve all sequencing problems, such as compressions and repeats; all regions were covered by sequence from more than one m13 subclone. FEATURES Qualifiers source /organism="Citrobacter koseri ATCC BAA-895" /mol_type="genomic DNA" /strain="ATCC BAA-895" /db_xref="ATCC:BAA-895" /db_xref="taxon:290338" protein /locus_tag="CKO_00225" /inference="protein motif:HMMPfam:IPR008258" /inference="protein motif:HMMSmart:IPR001638" /inference="protein motif:ScanRegExp:IPR000189" /inference="similar to AA sequence:REFSEQ:NP_461502.1" /note="KEGG: eci:UTI89_C2878 9.1e-241 yfhD; putative periplasmic binding transport protein K01238; COG: COG4623 Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein" /transl_table=11 /db_xref="InterPro:IPR000189" /db_xref="InterPro:IPR001638" /db_xref="InterPro:IPR008258" BEGIN 1 MKKLKINYLF IGILTLLLAA ALWPSIPWFG KADNRIAAIQ SRGELRVSTI ESPLTYARIN 61 GKKYGLDYEL AQQFASYLGV KLKITVRQNI SQLFDDLDNG NADLLAAGLV YNSERVKNYQ 121 PGPTYYSVSQ QLVYRVGQYR PRTLATVNES QLSIAPGHVV VNDLQALKET KFPDLSWKVD 181 DKKGTAALLS EVISGNLDYT IADSVAISLF QRVHPELAVA LDVTDEQPVT WFSQLDDDNT 241 LSAALLDFFN TINEDGSLAR MEEKYLGHGD DFDYVDTRTF LRAVDGVLPD LQPLFEKYAQ 301 EIDWRLLAAI SYQESHWDPL ATSPTGVRGL MMLTKNTAQS LGLTDRTDAE QSISGGVRYI 361 QDMMGKVPET VPEEERIWFA LAAYNMGYAH MLDARALTVK TRGNPDSWAD VKQRLPLLSQ 421 KQYYSKLTYG YARGHEAYAY VENIRKYQIS LVGYLQEKEK QAAEAIKLAQ DYPAVSPAEF 481 DTETFPFSSF LSQPSSNYLS HSPSLPFSLK KKDEN //