LOCUS ABU79351.1 649 aa PRT BCT 31-JAN-2014
DEFINITION Cronobacter sakazakii ATCC BAA-894 hypothetical protein protein.
ACCESSION CP000783-4041
PROTEIN_ID ABU79351.1
SOURCE Cronobacter sakazakii ATCC BAA-894
ORGANISM Cronobacter sakazakii ATCC BAA-894
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
Enterobacteriaceae; Cronobacter.
REFERENCE 1 (bases 1 to 4368373)
AUTHORS Kucerova,E., Clifton,S.W., Xia,X.Q., Long,F., Porwollik,S.,
Fulton,L., Fronick,C., Minx,P., Kyung,K., Warren,W., Fulton,R.,
Feng,D., Wollam,A., Shah,N., Bhonagiri,V., Nash,W.E.,
Hallsworth-Pepin,K., Wilson,R.K., McClelland,M. and Forsythe,S.J.
TITLE Genome sequence of Cronobacter sakazakii BAA-894 and comparative
genomic hybridization analysis with other Cronobacter species
JOURNAL PLoS ONE 5 (3), E9556 (2010)
PUBMED 20221447
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 4368373)
AUTHORS McClelland,M., Sanderson,E.K., Porwollik,S., Spieth,J.,
Clifton,W.S., Fulton,B., Wollam,A., Shah,N., Pepin,K.,
Bhonagiri,V., Nash,W., Johnson,M., Thiruvilangam,P. and Wilson,R.
TITLE Direct Submission
JOURNAL Submitted (25-JUL-2007) Genetics, Genome Sequencing Center, 4444
Forest Park Parkway, St. Louis, MO 63108, USA
COMMENT C. sakazakii--Cronobacter sakazakii is rarely encountered in
clinical specimens, and is more prevalent in the environment and in
food. However, Enterobacter sakazakii is strongly implicated in
food borne diseases causing severe meningitis or enteritis,
especially in neonates and infants (Nazarowec-White and Farber, Int
J FoodMicrobiol. 1997 Feb;34(2):103-13).
The strain of Enterobacter sakazakii being sequenced was isolated
from powdered milk formula fed to a hospitalized neonate that
developed an infection (Centers for Disease Control and
Prevention). It is available from the American Type Culture
Collection as ATCC BAA-894 or from the Salmonella Genetic Stock
Centre as SGSC4695. The genome was sequenced to 8X coverage, using
plasmid and fosmid libraries, and was finished to an error rate of
less than 1 per 10,000 bases. Automated annotation was performed
and manual annotation will continue in the labs of Michael
McClelland and Kenneth Sanderson. The National Institute of Allergy
and Infectious Diseases (NIAID), National Institutes of Health
(NIH) has funded this project.
Coding sequences below are predicted using GeneMark v3.3 and
Glimmer2 v2.13. Intergenic regions not spanned by GeneMark and
Glimmer2 were blasted against NCBI's non-redundant (NR) database
and predictions generated based on protein alignments. RNA genes
were determined using tRNAscan-SE 1.23 or Rfam v8.0. This sequence
was finished as follows unless otherwise noted: all regions were
double stranded, sequenced with an alternate chemistries or covered
by high quality data(i.e., phred quality >=30);an attempt was made
to resolve all sequencing problems, such as compressions and
repeats; all regionswere covered by sequence from more than one m13
subclone.
FEATURES Qualifiers
source /organism="Cronobacter sakazakii ATCC BAA-894"
/mol_type="genomic DNA"
/strain="ATCC BAA-894"
/culture_collection="ATCC:BAA-894"
/db_xref="taxon:290339"
protein /locus_tag="ESA_04170"
/inference="similar to AA sequence:REFSEQ:ZP_00415287.1"
/note="Psort location: Cytoplasmic, score:8.96"
/transl_table=11
BEGIN
1 MTRVVHAHDA LGVGKLKHHV GHQIAFREQA RTRRVIHIRA DLARNPAGER LNAVGFVAQR
61 AELLLEQHGF ETRQVIFQTF FAVGVEEELR IRQTRTHHFF VTGDNLARIF GLDIRHKDKV
121 RQQLAVGTVH REILLVAFHG IDQRFRRHRQ EFLFEFRGQY HRPFHQRGDF FKQAVAQIGI
181 AADFRRCGFG VLLNFSFTRV VVGDDFAALQ QDLRVLIGVV DSEFRLAHKA MTTDHAVGLN
241 TKNSRRQHFI VQQQRHGVNR AHELHVGRAP AHQFRNRQFR QRGAHHVRQQ RLRAFAFHMG
301 AIHQPLAFVG DQTLRLIDSN AAAARPAFRR FARVALCVKR RRDRRATFFD FAIRLRRRQI
361 RHFQRQTTRR RKPAHDAVGQ ARRVQFSSKV RSEGFRQAAQ GFWWQLFSAN LHQKSFLRHG
421 SLLFISVAHR EAQRLAGGVV GFGHGFGQRA DTQNVALALG HRDSLARVQQ VEAVGGFQNT
481 FISRQRQRVF QRQQLLSFFF VLLKAGEQEI HVGVFEVIGG LLHFVLMEHI AVSGFAKRAI
541 APDQVINAVH ALNVHGETLK AVSDFTGHRF ALQAADLLEV SELRHFHAVQ PHFPAEAPGA
601 ERRVFPVVFD EANIMNGRVH TQLFKRPQVQ LLNIVRRRLD DHLELVVVL
//