LOCUS ABU78928.1 753 aa PRT BCT 31-JAN-2014 DEFINITION Cronobacter sakazakii ATCC BAA-894 hypothetical protein protein. ACCESSION CP000783-3619 PROTEIN_ID ABU78928.1 SOURCE Cronobacter sakazakii ATCC BAA-894 ORGANISM Cronobacter sakazakii ATCC BAA-894 Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Cronobacter. REFERENCE 1 (bases 1 to 4368373) AUTHORS Kucerova,E., Clifton,S.W., Xia,X.Q., Long,F., Porwollik,S., Fulton,L., Fronick,C., Minx,P., Kyung,K., Warren,W., Fulton,R., Feng,D., Wollam,A., Shah,N., Bhonagiri,V., Nash,W.E., Hallsworth-Pepin,K., Wilson,R.K., McClelland,M. and Forsythe,S.J. TITLE Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species JOURNAL PLoS ONE 5 (3), E9556 (2010) PUBMED 20221447 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 4368373) AUTHORS McClelland,M., Sanderson,E.K., Porwollik,S., Spieth,J., Clifton,W.S., Fulton,B., Wollam,A., Shah,N., Pepin,K., Bhonagiri,V., Nash,W., Johnson,M., Thiruvilangam,P. and Wilson,R. TITLE Direct Submission JOURNAL Submitted (25-JUL-2007) Genetics, Genome Sequencing Center, 4444 Forest Park Parkway, St. Louis, MO 63108, USA COMMENT C. sakazakii--Cronobacter sakazakii is rarely encountered in clinical specimens, and is more prevalent in the environment and in food. However, Enterobacter sakazakii is strongly implicated in food borne diseases causing severe meningitis or enteritis, especially in neonates and infants (Nazarowec-White and Farber, Int J FoodMicrobiol. 1997 Feb;34(2):103-13). The strain of Enterobacter sakazakii being sequenced was isolated from powdered milk formula fed to a hospitalized neonate that developed an infection (Centers for Disease Control and Prevention). It is available from the American Type Culture Collection as ATCC BAA-894 or from the Salmonella Genetic Stock Centre as SGSC4695. The genome was sequenced to 8X coverage, using plasmid and fosmid libraries, and was finished to an error rate of less than 1 per 10,000 bases. Automated annotation was performed and manual annotation will continue in the labs of Michael McClelland and Kenneth Sanderson. The National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH) has funded this project. Coding sequences below are predicted using GeneMark v3.3 and Glimmer2 v2.13. Intergenic regions not spanned by GeneMark and Glimmer2 were blasted against NCBI's non-redundant (NR) database and predictions generated based on protein alignments. RNA genes were determined using tRNAscan-SE 1.23 or Rfam v8.0. This sequence was finished as follows unless otherwise noted: all regions were double stranded, sequenced with an alternate chemistries or covered by high quality data(i.e., phred quality >=30);an attempt was made to resolve all sequencing problems, such as compressions and repeats; all regionswere covered by sequence from more than one m13 subclone. FEATURES Qualifiers source /organism="Cronobacter sakazakii ATCC BAA-894" /mol_type="genomic DNA" /strain="ATCC BAA-894" /culture_collection="ATCC:BAA-894" /db_xref="taxon:290339" protein /locus_tag="ESA_03731" /inference="protein motif:BlastProDom:IPR002629" /inference="protein motif:HMMPfam:IPR002629" /inference="protein motif:HMMPfam:IPR013215" /inference="protein motif:HMMTigr:IPR006276" /inference="protein motif:superfamily:IPR011254" /note="KEGG: ecj:JW3805 0. metE; 5-methyltetrahydropteroyltriglutamate- homocysteine S-methyltransferase K00549; COG: COG0620 Methionine synthase II (cobalamin-independent); Psort location: Cytoplasmic, score:8.96" /transl_table=11 /db_xref="InterPro:IPR002629" /db_xref="InterPro:IPR006276" /db_xref="InterPro:IPR011254" /db_xref="InterPro:IPR013215" BEGIN 1 MTIHNHTLGF PRVGLRRELK KAQESYWAGK STREELLAVG RELRARHWEQ QKAAGIDLLP 61 VGDFAWYDHV LTTSLLLGNV PARHQNADGT VDIDTLFRIG RGRAPTGEPA AAAEMTKWFN 121 TNYHYMVPEF TKGQQFSLTW TQLLDEVDEA LALGHHVKPV LLGPVTYLWL GKVKGEQFDR 181 LSLLNDILPV YQQVIAELAK RGIQWVQIDE PALVLELPQA WLDAFKPAYE ALKGQTKLLL 241 TTYFEGVSDN LDTITALPVQ GLHVDLVHGH DDVNELHRRL PQEWLLSAGV INGRNVWRAD 301 LTEKYAQLKA IAGQRELWVG SSCSLLHSPI DLSVETRLDA EVKSWFAFAL QKCEELALLR 361 DALNSGDTTK IEQWSAPIQA RKHSARVHNP AVEQRLKAIT PQDSQRAHAY PVRAEAQRAR 421 FNLPAWPTTT IGSFPQTTEI RGLRLDFKKG NLDAANYRTG IAEHIKQAIA EQERLGLDVL 481 VHGEAERNDM VEYFGEHLDG FVFTQNGWVQ SYGSRCVKPP VVIGDISRPA PITVEWAKYA 541 QSLTDKPVKG MLTGPVTILC WSFPREDVSR ETIAKQIALA LRDEVADLEA AGIGIIQIDE 601 PALREGLPLK RSDWDAYLAW GVEAFRLNAA VAKDDTQIHT HMCYCEFNDI MDSIAALDAD 661 VITIETSRSD MELLESFEEF EYPNEIGPGV YDIHSPNVPD VAWIEALLKK AAQRIPQERL 721 WVNPDCGLKT RGWPETRAAL ANMVKAAQNL RQA //