LOCUS ABU77790.1 618 aa PRT BCT 31-JAN-2014 DEFINITION Cronobacter sakazakii ATCC BAA-894 hypothetical protein protein. ACCESSION CP000783-2481 PROTEIN_ID ABU77790.1 SOURCE Cronobacter sakazakii ATCC BAA-894 ORGANISM Cronobacter sakazakii ATCC BAA-894 Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Cronobacter. REFERENCE 1 (bases 1 to 4368373) AUTHORS Kucerova,E., Clifton,S.W., Xia,X.Q., Long,F., Porwollik,S., Fulton,L., Fronick,C., Minx,P., Kyung,K., Warren,W., Fulton,R., Feng,D., Wollam,A., Shah,N., Bhonagiri,V., Nash,W.E., Hallsworth-Pepin,K., Wilson,R.K., McClelland,M. and Forsythe,S.J. TITLE Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species JOURNAL PLoS ONE 5 (3), E9556 (2010) PUBMED 20221447 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 4368373) AUTHORS McClelland,M., Sanderson,E.K., Porwollik,S., Spieth,J., Clifton,W.S., Fulton,B., Wollam,A., Shah,N., Pepin,K., Bhonagiri,V., Nash,W., Johnson,M., Thiruvilangam,P. and Wilson,R. TITLE Direct Submission JOURNAL Submitted (25-JUL-2007) Genetics, Genome Sequencing Center, 4444 Forest Park Parkway, St. Louis, MO 63108, USA COMMENT C. sakazakii--Cronobacter sakazakii is rarely encountered in clinical specimens, and is more prevalent in the environment and in food. However, Enterobacter sakazakii is strongly implicated in food borne diseases causing severe meningitis or enteritis, especially in neonates and infants (Nazarowec-White and Farber, Int J FoodMicrobiol. 1997 Feb;34(2):103-13). The strain of Enterobacter sakazakii being sequenced was isolated from powdered milk formula fed to a hospitalized neonate that developed an infection (Centers for Disease Control and Prevention). It is available from the American Type Culture Collection as ATCC BAA-894 or from the Salmonella Genetic Stock Centre as SGSC4695. The genome was sequenced to 8X coverage, using plasmid and fosmid libraries, and was finished to an error rate of less than 1 per 10,000 bases. Automated annotation was performed and manual annotation will continue in the labs of Michael McClelland and Kenneth Sanderson. The National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH) has funded this project. Coding sequences below are predicted using GeneMark v3.3 and Glimmer2 v2.13. Intergenic regions not spanned by GeneMark and Glimmer2 were blasted against NCBI's non-redundant (NR) database and predictions generated based on protein alignments. RNA genes were determined using tRNAscan-SE 1.23 or Rfam v8.0. This sequence was finished as follows unless otherwise noted: all regions were double stranded, sequenced with an alternate chemistries or covered by high quality data(i.e., phred quality >=30);an attempt was made to resolve all sequencing problems, such as compressions and repeats; all regionswere covered by sequence from more than one m13 subclone. FEATURES Qualifiers source /organism="Cronobacter sakazakii ATCC BAA-894" /mol_type="genomic DNA" /strain="ATCC BAA-894" /culture_collection="ATCC:BAA-894" /db_xref="taxon:290339" protein /locus_tag="ESA_02545" /inference="protein motif:BlastProDom:IPR001127" /inference="protein motif:BlastProDom:IPR001996" /inference="protein motif:HMMPfam:IPR001127" /inference="protein motif:HMMPfam:IPR001996" /inference="protein motif:HMMPfam:IPR003352" /inference="protein motif:HMMTigr:IPR001127" /inference="protein motif:HMMTigr:IPR011297" /inference="protein motif:ScanRegExp:IPR001127" /inference="protein motif:superfamily:IPR011055" /inference="similar to AA sequence:INSD:CAA84286.1" /note="KEGG: bsu:BG10934 6.0e-157 bglP, sytA, n17C; PTS beta-glucoside-specific enzyme IIBCA component K02755:K02756:K02757; COG: COG2190 Phosphotransferase system IIA components; Psort location: CytoplasmicMembrane, score:10.00" /transl_table=11 /db_xref="InterPro:IPR001127" /db_xref="InterPro:IPR001996" /db_xref="InterPro:IPR003352" /db_xref="InterPro:IPR011055" /db_xref="InterPro:IPR011297" BEGIN 1 MNYPHTARQI ITLLGGEANI LSLYHCITRL RFSLVSLEKV DRAALEHLDG VMGVNLSGDQ 61 FQVIIGSEVA PLCQAILAQL PGLEAKKAAA PPKRRNPISM VLEGLAGIFS PIIPAIAGAG 121 ILKGVLSLCL ALGWVQASNQ TYQILMAISD GVFFFMPLVL AFSAGNKFGA NPYVAVALAA 181 TLFHPMLTTL LKSGAPVAFL GLPVSSVSYA SSVIPILLAV LLLSYVERFI DRLMPAVLKT 241 MFVPLLSLVI VAPVTLIAIG PAGILLGNAL SGGIIWLVAN MGWLAGVIVG GTLSLMIITG 301 MHYVLVPIVI NNISKLGYDP FKILFYVANM GQAGAAFGVF LRARNKKIKT LALSTSFSAA 361 MGITEPAMYG INIRFKRPFA AALIGGACGG AFAMALGVKT YAFALSGIPG IPALVGPTFL 421 WALASLAIAF CAAALITVML GFEEPAEAPA EPEVVAGLSP VALAREEQLF APVSGRLTPL 481 SSLSDPVFAD EIFGKGIAIV PESGELLSPV NGRIEAIFDS NHALTLRSDC GAEVLIHIGI 541 DTVKLGGQHF TRHVENGAFV EAGQPLISFD LAALNALHID PSVIVIVTNT HCYGEISPLK 601 QGDVAPREAF LKLTAAAA //