LOCUS       AAI56683.1               730 aa    PRT              SYN 23-JAN-2008
DEFINITION  synthetic construct guanylate cyclase 1, soluble, alpha 2 protein.
ACCESSION   BC156682-1
PROTEIN_ID  AAI56683.1
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Strausberg,R.L., Feingold,E.A., Grouse,L.H., Derge,J.G.,
            Klausner,R.D., Collins,F.S., Wagner,L., Shenmen,C.M., Schuler,G.D.,
            Altschul,S.F., Zeeberg,B., Buetow,K.H., Schaefer,C.F., Bhat,N.K.,
            Hopkins,R.F., Jordan,H., Moore,T., Max,S.I., Wang,J., Hsieh,F.,
            Diatchenko,L., Marusina,K., Farmer,A.A., Rubin,G.M., Hong,L.,
            Stapleton,M., Soares,M.B., Bonaldo,M.F., Casavant,T.L.,
            Scheetz,T.E., Brownstein,M.J., Usdin,T.B., Toshiyuki,S.,
            Carninci,P., Prange,C., Raha,S.S., Loquellano,N.A., Peters,G.J.,
            Abramson,R.D., Mullahy,S.J., Bosak,S.A., McEwan,P.J.,
            McKernan,K.J., Malek,J.A., Gunaratne,P.H., Richards,S.,
            Worley,K.C., Hale,S., Garcia,A.M., Gay,L.J., Hulyk,S.W.,
            Villalon,D.K., Muzny,D.M., Sodergren,E.J., Lu,X., Gibbs,R.A.,
            Fahey,J., Helton,E., Ketteman,M., Madan,A., Rodrigues,S.,
            Sanchez,A., Whiting,M., Madan,A., Young,A.C., Shevchenko,Y.,
            Bouffard,G.G., Blakesley,R.W., Touchman,J.W., Green,E.D.,
            Dickson,M.C., Rodriguez,A.C., Grimwood,J., Schmutz,J., Myers,R.M.,
            Butterfield,Y.S., Krzywinski,M.I., Skalska,U., Smailus,D.E.,
            Schnerch,A., Schein,J.E., Jones,S.J. and Marra,M.A.
  CONSRTM   Mammalian Gene Collection Program Team
  TITLE     Generation and initial analysis of more than 15,000 full-length
            human and mouse cDNA sequences
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002)
   PUBMED   12477932
REFERENCE   2  (bases 1 to 2254)
  CONSRTM   NIH MGC Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2007) National Institutes of Health, Mammalian
            Gene Collection (MGC), Bethesda, MD 20892-2590, USA
  REMARK    NIH-MGC Project URL: http://mgc.nci.nih.gov
COMMENT     This clone is a part of a collection of full-length cDNA clones
            generated by Mammalian Gene Collection project. DNA synthesis was
            used to prepare the protein coding sequence (ORF) and flanking
            sequences, permitting directional cloning into the Gateway Entry
            vector, pENTR223.1 (Details are described in
            http://mgc.nci.nih.gov/Vectors/prot_pENTR223.1).
            
            Clone structure of the ORF and flanking sequences is as follows:
            5'- gtac aaa aaa gca gaa gGG CCG TCA AGG CCC ACC 5'ORF3' TAA GGC
            CTC ATG Ggc cca gct ttc ttg-3'.
            
            This clone is also a part of ORFeome collaboration clones
            (http://www.orfeomecollaboration.org/).
            Contact: MGC help desk
            Email: cgapbs-r@mail.nih.gov
            Tissue Procurement: Geneart
            cDNA Library Preparation: GeneArt
            cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL)
            DNA Sequencing by: Geneart - the gene of your choice:
            www.geneart.com
            
            Clone distribution: MGC clone distribution information can be found
            through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov
            Series: IRCW Plate: 12 Row: e Column: 12.
FEATURES             Qualifiers
     source          /organism="synthetic construct"
                     /mol_type="other DNA"
                     /db_xref="taxon:32630"
                     /clone="MGC:190368 IMAGE:100062120"
                     /clone_lib="NIH_MGC_426"
                     /lab_host="DH10B"
                     /focus
                     /note="Vector: pENTR223.1 with stop codon"
     source          /organism="Mus musculus"
                     /mol_type="other DNA"
                     /db_xref="taxon:10090"
     protein         /gene="Gucy1a2"
                     /gene_synonym="9430053E15"
                     /db_xref="GeneID:234889"
                     /db_xref="MGI:MGI:2660877"
BEGIN
        1 MSRRKISSES FSSLSSDYLE TSPEEEGECP LSRLCWNGSR SPPGPPGSRE AAKAATSAPA
       61 ASAAAASAAV AAEYKRAQRR GRVNLDSLGE SISLLTAPLP QTIHMTLKRT LQYYEHQVIG
      121 YRDAEKNFHN ISKRCSYADH SNKEEIEDVS GILQCTANVL GLQFQEIQER FGEEFFKICF
      181 DENERVLRAV GSTLQDFFNG FDALLEHIRT SFGKQATLES SSFLCKELPE GTLMLHYFHP
      241 HHTVGFAMLG MIKAAGKRIY HLNVEVEQIE NEKLCFDGSN PSNCSCLTFL IKECETTQIT
      301 KDNPQGTSQV PADLRISINT FCRAFPFHLM FDSNMVVLQL GEGLRKQLRY DTHRALKFED
      361 CFEIVSPMIN ATFDRVLLRL STPFVIRTKP EASGTENEDK VMEIKGQMIH VPESSAILFL
      421 GSPCVDKLDE LMGRGLHLSD IPIHDATRDV ILVGEQAKAQ DGLKKRMDKL KATLERTHQA
      481 LEEEKKKTVD LLYSIFPGDV AQQLWQGQQV QARKFDDVTM LFSDIVGFTA ICAQCTPMQV
      541 ISMLNELYTR FDHQCGLLDI YKVETIGDAY CVASGLHRKS LCHAKPIALM ALKMMELSEE
      601 VLTPDGKAIQ MRIGIHSGSV LAGVVGVRMP RYCLFGNNVT LASKFESGSH PRRINVSPTT
      661 YQLLKREDSF TFIPRSREEL PDNFPKEIPG VCYFLELRTD PKPPKPSLSS SRIKKVSYNI
      721 GTMFLRETSL
//