LOCUS AAC73447.1 1024 aa PRT BCT 17-NOV-2022 DEFINITION Escherichia coli str. K-12 substr. MG1655 beta-galactosidase protein. ACCESSION U00096-338 PROTEIN_ID AAC73447.1 SOURCE Escherichia coli str. K-12 substr. MG1655 ORGANISM Escherichia coli str. K-12 substr. MG1655 Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 (bases 1 to 4641652) AUTHORS Blattner,F.R., Plunkett,G. III, Bloch,C.A., Perna,N.T., Burland,V., Riley,M., Collado-Vides,J., Glasner,J.D., Rode,C.K., Mayhew,G.F., Gregor,J., Davis,N.W., Kirkpatrick,H.A., Goeden,M.A., Rose,D.J., Mau,B. and Shao,Y. TITLE The complete genome sequence of Escherichia coli K-12 JOURNAL Science 277 (5331), 1453-1462 (1997) PUBMED 9278503 REFERENCE 2 (bases 1 to 4641652) AUTHORS Hayashi,K., Morooka,N., Yamamoto,Y., Fujita,K., Isono,K., Choi,S., Ohtsubo,E., Baba,T., Wanner,B.L., Mori,H. and Horiuchi,T. TITLE Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110 JOURNAL Mol. Syst. Biol. 2, 2006 (2006) PUBMED 16738553 REFERENCE 3 (bases 1 to 4641652) AUTHORS Riley,M., Abe,T., Arnaud,M.B., Berlyn,M.K., Blattner,F.R., Chaudhuri,R.R., Glasner,J.D., Horiuchi,T., Keseler,I.M., Kosuge,T., Mori,H., Perna,N.T., Plunkett,G. III, Rudd,K.E., Serres,M.H., Thomas,G.H., Thomson,N.R., Wishart,D. and Wanner,B.L. TITLE Escherichia coli K-12: a cooperatively developed annotation snapshot--2005 JOURNAL Nucleic Acids Res. 34 (1), 1-9 (2006) PUBMED 16397293 REMARK Publication Status: Online-Only REFERENCE 4 (bases 1 to 4641652) AUTHORS Arnaud,M., Berlyn,M.K.B., Blattner,F.R., Galperin,M.Y., Glasner,J.D., Horiuchi,T., Kosuge,T., Mori,H., Perna,N.T., Plunkett,G. III, Riley,M., Rudd,K.E., Serres,M.H., Thomas,G.H. and Wanner,B.L. TITLE Workshop on Annotation of Escherichia coli K-12 JOURNAL Unpublished REMARK Woods Hole, Mass., on 14-18 November 2003 (sequence corrections) REFERENCE 5 (bases 1 to 4641652) AUTHORS Glasner,J.D., Perna,N.T., Plunkett,G. III, Anderson,B.D., Bockhorst,J., Hu,J.C., Riley,M., Rudd,K.E. and Serres,M.H. TITLE ASAP: Escherichia coli K-12 strain MG1655 version m56 JOURNAL Unpublished REMARK ASAP download 10 June 2004 (annotation updates) REFERENCE 6 (bases 1 to 4641652) AUTHORS Hayashi,K., Morooka,N., Mori,H. and Horiuchi,T. TITLE A more accurate sequence comparison between genomes of Escherichia coli K12 W3110 and MG1655 strains JOURNAL Unpublished REMARK GenBank accessions AG613214 to AG613378 (sequence corrections) REFERENCE 7 (bases 1 to 4641652) AUTHORS Perna,N.T. TITLE Escherichia coli K-12 MG1655 yqiK-rfaE intergenic region, genomic sequence correction JOURNAL Unpublished REMARK GenBank accession AY605712 (sequence corrections) REFERENCE 8 (bases 1 to 4641652) AUTHORS Rudd,K.E. TITLE A manual approach to accurate translation start site annotation: an E. coli K-12 case study JOURNAL Unpublished REFERENCE 9 (bases 1 to 4641652) AUTHORS Blattner,F.R. and Plunkett,G. III. TITLE Direct Submission JOURNAL Submitted (16-JAN-1997) Laboratory of Genetics, University of Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA REFERENCE 10 (bases 1 to 4641652) AUTHORS Blattner,F.R. and Plunkett,G. III. TITLE Direct Submission JOURNAL Submitted (02-SEP-1997) Laboratory of Genetics, University of Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA REFERENCE 11 (bases 1 to 4641652) AUTHORS Plunkett,G. III. TITLE Direct Submission JOURNAL Submitted (13-OCT-1998) Laboratory of Genetics, University of Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA REFERENCE 12 (bases 1 to 4641652) AUTHORS Plunkett,G. III. TITLE Direct Submission JOURNAL Submitted (10-JUN-2004) Laboratory of Genetics, University of Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA REMARK Sequence update by submitter REFERENCE 13 (bases 1 to 4641652) AUTHORS Plunkett,G. III. TITLE Direct Submission JOURNAL Submitted (07-FEB-2006) Laboratory of Genetics, University of Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA REMARK Protein updates by submitter REFERENCE 14 (bases 1 to 4641652) AUTHORS Rudd,K.E. TITLE Direct Submission JOURNAL Submitted (24-APR-2007) Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, 118 Gautier Bldg., Miami, FL 33136, USA REMARK Annotation update from ecogene.org as a multi-database collaboration REFERENCE 15 (bases 1 to 4641652) AUTHORS Rudd,K.E. TITLE Direct Submission JOURNAL Submitted (06-FEB-2013) Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, 118 Gautier Bldg., Miami, FL 33136, USA REMARK Sequence update by submitter REFERENCE 16 (bases 1 to 4641652) AUTHORS Blattner,F.R. and Plunkett,G. III. TITLE Direct Submission JOURNAL Submitted (26-SEP-2013) Laboratory of Genetics, University of Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA REMARK Sequence update by submitter REFERENCE 17 (bases 1 to 4641652) AUTHORS Blattner,F.R. and Plunkett,G. III. TITLE Direct Submission JOURNAL Submitted (15-NOV-2013) Laboratory of Genetics, University of Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA REMARK Protein update by submitter REFERENCE 18 (bases 1 to 4641652) AUTHORS Blattner,F.R. and Plunkett,G. III. TITLE Direct Submission JOURNAL Submitted (30-JUL-2014) Laboratory of Genetics, University of Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA REMARK Protein update by submitter COMMENT On Sep 26, 2013 this sequence version replaced U00096.2. On September 24, 2018 changes were made to proteins and annotation On September 23, 2020 changes were made to proteins and annotation On March 7, 2022 changes were made to the proteins and annotation. Current U00096 annotation updates are derived from EcoCyc https://ecocyc.org/. Suggestions for updates can be sent to biocyc-support@ai.sri.com. These updates are being generated from a collaboration that includes EcoCyc, the University of Wisconsin, UniProtKB/Swiss-Prot, and the National Center for Biotechnology Information (NCBI). FEATURES Qualifiers source /organism="Escherichia coli str. K-12 substr. MG1655" /mol_type="genomic DNA" /strain="K-12" /sub_strain="MG1655" /db_xref="taxon:511145" protein /gene="lacZ" /locus_tag="b0344" /gene_synonym="ECK0341" /EC_number="3.2.1.108" /transl_table=11 /db_xref="UniProtKB/Swiss-Prot:P00722" /db_xref="ASAP:ABE-0001183" /db_xref="ECOCYC:EG10527" BEGIN 1 MTMITDSLAV VLQRRDWENP GVTQLNRLAA HPPFASWRNS EEARTDRPSQ QLRSLNGEWR 61 FAWFPAPEAV PESWLECDLP EADTVVVPSN WQMHGYDAPI YTNVTYPITV NPPFVPTENP 121 TGCYSLTFNV DESWLQEGQT RIIFDGVNSA FHLWCNGRWV GYGQDSRLPS EFDLSAFLRA 181 GENRLAVMVL RWSDGSYLED QDMWRMSGIF RDVSLLHKPT TQISDFHVAT RFNDDFSRAV 241 LEAEVQMCGE LRDYLRVTVS LWQGETQVAS GTAPFGGEII DERGGYADRV TLRLNVENPK 301 LWSAEIPNLY RAVVELHTAD GTLIEAEACD VGFREVRIEN GLLLLNGKPL LIRGVNRHEH 361 HPLHGQVMDE QTMVQDILLM KQNNFNAVRC SHYPNHPLWY TLCDRYGLYV VDEANIETHG 421 MVPMNRLTDD PRWLPAMSER VTRMVQRDRN HPSVIIWSLG NESGHGANHD ALYRWIKSVD 481 PSRPVQYEGG GADTTATDII CPMYARVDED QPFPAVPKWS IKKWLSLPGE TRPLILCEYA 541 HAMGNSLGGF AKYWQAFRQY PRLQGGFVWD WVDQSLIKYD ENGNPWSAYG GDFGDTPNDR 601 QFCMNGLVFA DRTPHPALTE AKHQQQFFQF RLSGQTIEVT SEYLFRHSDN ELLHWMVALD 661 GKPLASGEVP LDVAPQGKQL IELPELPQPE SAGQLWLTVR VVQPNATAWS EAGHISAWQQ 721 WRLAENLSVT LPAASHAIPH LTTSEMDFCI ELGNKRWQFN RQSGFLSQMW IGDKKQLLTP 781 LRDQFTRAPL DNDIGVSEAT RIDPNAWVER WKAAGHYQAE AALLQCTADT LADAVLITTA 841 HAWQHQGKTL FISRKTYRID GSGQMAITVD VEVASDTPHP ARIGLNCQLA QVAERVNWLG 901 LGPQENYPDR LTAACFDRWD LPLSDMYTPY VFPSENGLRC GTRELNYGPH QWRGDFQFNI 961 SRYSQQQLME TSHRHLLHAE EGTWLNIDGF HMGIGGDDSW SPSVSAEFQL SAGRYHYQLV 1021 WCQK //