LOCUS       AAC53682.1               345 aa    PRT              SYN 13-MAR-1997
DEFINITION  synthetic construct hygromycin B phosphotransferase protein.
ACCESSION   U40398-1
PROTEIN_ID  AAC53682.1
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Yin,D.X. and Schimke,R.T.
  TITLE     BCL-2 expression delays drug-induced apoptosis but does not
            increase clonogenic survival after drug treatment in HeLa cells
  JOURNAL   Cancer Res. 55 (21), 4922-4928 (1995)
   PUBMED   7585531
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   Yin,D.X., Zhu,L., Zahl,N. and Zhou,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-1995) Dong X. Yin, CLONTECH Laboratories, 1020 E.
            Meadow Circle, Palo Alto, CA 94303, USA
COMMENT     This plasmid will be available from CLONTECH January 1996.  Inquire
            at 1-800-662-clon.
FEATURES             Qualifiers
     source          /organism="synthetic construct"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:32630"
     protein         /gene="hyg"
                     /note="driven by HSV tk promoter; confers hygromycin
                     resistance; An out-of-frame translation intiation codon
                     has been removed from the hygromycin resistance gene so
                     that the translation efficiency is improved by
                     approximately 5 fold. Because this is an efficiently
                     translated hygromycin resistance gene driven by an
                     enhancer-less promoter, it is the selection marker of
                     choice to be used with many inducible gene expression
                     systems, especially the tetracyline-controlled gene
                     expression systems"
                     /transl_table=11
BEGIN
        1 MKKPELTATS VEKFLIEKFD SVSDLMQLSE GEESRAFSFD VGGRGYVLRV NSCADGFYKD
       61 RYVYRHFASA ALPIPEVLDI GEFSESLTYC ISRRAQGVTL QDLPETELPA VLQPVAEAMD
      121 AIAAADLSQT SGFGPFGPQG IGQYTTWRDF ICAIADPHVY HWQTVMDDTV SASVAQALDE
      181 LMLWAEDCPE VRHLVHADFG SNNVLTDNGR ITAVIDWSEA MFGDSQYEVA NIFFWRPWLA
      241 CMEQQTRYFE RRHPELAGSP RLRAYMLRIG LDQLYQSLVD GNFDDAAWAQ GRCDAIVRSG
      301 AGTVGRTQIA RRSAAVWTDG CVEVLADSGN RRPSTRGDRE MGEAN
//