LOCUS       AAA58485.1               187 aa    PRT              HUM 31-DEC-1994
DEFINITION  Homo sapiens DHFR protein.
ACCESSION   J00140-1
PROTEIN_ID  AAA58485.1
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 137)
  AUTHORS   Morandi,C., Masters,J.N., Mottes,M. and Attardi,G.
  TITLE     Multiple forms of human dihydrofolate reductase messenger RNA.
            Cloning and expression in Escherichia coli of their DNA coding
            sequence
  JOURNAL   J. Mol. Biol. 156 (3), 583-607 (1982)
   PUBMED   6750132
REFERENCE   2  (bases 43 to 606)
  AUTHORS   Masters,J.N. and Attardi,G.
  TITLE     The nucleotide sequence of the cDNA coding for the human
            dihydrofolic acid reductase
  JOURNAL   Gene 21 (1-2), 59-63 (1983)
   PUBMED   6687716
REFERENCE   3  (bases 21 to 979)
  AUTHORS   Masters,J.N., Yang,J.K., Cellini,A. and Attardi,G.
  TITLE     A human dihydrofolate reductase pseudogene and its relationship to
            the multiple forms of specific messenger RNA
  JOURNAL   J. Mol. Biol. 167 (1), 23-36 (1983)
   PUBMED   6306253
COMMENT     Original source text: human cdna to mrna, clones phd84, phd80,
            phd83 and phd41 (see comment).
            [1] deduced dhfr coding sequence by its homology to the mouse dhfr
            dna sequence (see musfol). plasmid phd84 confers upon e. coli
            resistance to trimethoprin. [2] found an 89% homology (98%
            functional homology) between the human dhfr reading frame and the
            mouse dhfr coding sequence. [3] shows sequence of a human dhfr
            pseudogene (see humfolpsd1).
            Complete source information:
            human cdna to methotrexate resistant cell variant 6a3 mrna. [1]:
            plasmid phd84; [2]: combined sequences from plasmids phd84, phd80,
            phd83; [3]: combined sequences from plasmids phd84, phd80, phd83
            and phd41.
FEATURES             Qualifiers
     source          /db_xref="H-InvDB:HIT000191050"
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
     protein         /gene="DHFR"
                     /note="dihydrofolate reductase"
BEGIN
        1 MVGSLNCIVA VSQNMGIGKN GDLPWPPLRN EFRYFQRMTT TSSVEGKQNL VIMGKKTWFS
       61 IPEKNRPLKG RINLVLSREL KEPPQGAHFL SRSLDDALKL TEQPELANKV DMVWIVGGSS
      121 VYKEAMNHPG HLKLFVTRIM QDFESDTFFP EIDLEKYKLL PEYPGVLSDV QEEKGIKYKF
      181 EVYEKND
//