LOCUS AAA24052.1 360 aa PRT BCT 15-NOV-2023 DEFINITION Escherichia coli lacI protein. ACCESSION J01636-1 PROTEIN_ID AAA24052.1 SOURCE Escherichia coli ORGANISM Escherichia coli Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 (bases 1243 to 1266) AUTHORS Gilbert,W. and Maxam,A. TITLE The nucleotide sequence of the lac operator JOURNAL Proc. Natl. Acad. Sci. U.S.A. 70 (12), 3581-3584 (1973) PUBMED 4587255 REFERENCE 2 (bases 1246 to 1308) AUTHORS Maizels,N.M. TITLE The nucleotide sequence of the lactose messenger ribonucleic acid transcribed from the UV5 promoter mutant of Escherichia coli JOURNAL Proc. Natl. Acad. Sci. U.S.A. 70 (12), 3585-3589 (1973) PUBMED 4587256 REFERENCE 3 (sites) AUTHORS Gilbert,W., Maizels,N. and Maxam,A. TITLE Sequences of controlling regions of the lactose operon JOURNAL Cold Spring Harb. Symp. Quant. Biol. 38, 845-855 (1974) PUBMED 4598642 REFERENCE 4 (sites) AUTHORS Gilbert,W., Gralla,J., Majors,A.J. and Maxam,A. TITLE Lactose operator sequences and the action of lac repressor JOURNAL (in) Sund,H. and Blauer,G. (Eds.); PROTEIN-LIGAND INTERACTIONS: 193-207; Walter de Gruyter, New York (1975) REFERENCE 5 (bases 1146 to 1282) AUTHORS Dickson,R.C., Abelson,J., Barnes,W.M. and Reznikoff,W.S. TITLE Genetic regulation: the Lac control region JOURNAL Science 187 (4171), 27-35 (1975) PUBMED 1088926 REFERENCE 6 (bases 1227 to 1271) AUTHORS Gilbert,W., Maxam,A. and Mirzabekov,A. TITLE Contacts between the lac repressor and DNA revealed by methylation JOURNAL (in) Kjeldgaard,N.C. and Maaloe,O. (Eds.); CONTROL OF RIBOSOME SYNTHESIS: 138-143; Academic Press, New York (1976) REFERENCE 7 (sites) AUTHORS Marians,K.J. and Brooker,J.D. TITLE Structure of the lactose operator JOURNAL Nature 260 (5549), 360-363 (1976) PUBMED 768781 REFERENCE 8 (bases 1242 to 1268) AUTHORS Heyneker,H.L., Shine,J., Goodman,H.M., Boyer,H.W., Rosenberg,J., Dickerson,R.E., Narang,S.A., Itakura,K., Lin,S. and Riggs,A.D. TITLE Synthetic lac operator DNA is functional in vivo JOURNAL Nature 263 (5580), 748-752 (1976) PUBMED 1069185 REFERENCE 9 (sites) AUTHORS Dickson,R.C., Abelson,J. and Johnson,P. TITLE Nucleotide sequence changes produced by mutations in the lac promoter of Escherichia coli JOURNAL J. Mol. Biol. 111 (1), 65-75 (1977) PUBMED 323498 REFERENCE 10 (bases 51 to 264) AUTHORS Steege,D.A. TITLE 5'-Terminal nucleotide sequence of Escherichia coli lactose repressor mRNA: features of translational initiation and reinitiation sites JOURNAL Proc. Natl. Acad. Sci. U.S.A. 74 (10), 4163-4167 (1977) PUBMED 337294 REFERENCE 11 (bases 1 to 81) AUTHORS Calos,M.P. TITLE DNA sequence for a low-level promoter of the lac repressor gene and an 'up' promoter mutation JOURNAL Nature 274 (5673), 762-765 (1978) PUBMED 355890 REFERENCE 12 (bases 49 to 1161) AUTHORS Farabaugh,P.J. TITLE Sequence of the lacI gene JOURNAL Nature 274 (5673), 765-769 (1978) PUBMED 355891 REFERENCE 13 (sites) AUTHORS Miller,J.H., Coulondre,C. and Farabaugh,P.J. TITLE Correlation of nonsense sites in the lacI gene with specific codons in the nucleotide sequence JOURNAL Nature 274 (5673), 770-775 (1978) PUBMED 355892 REFERENCE 14 (sites) AUTHORS Calos,M.P. and Miller,J.H. TITLE DNA sequence alteration resulting from a mutation impairing promoter function in the lac repressor gene JOURNAL Mol. Gen. Genet. 178 (1), 225-227 (1980) PUBMED 6770231 REFERENCE 15 (bases 4306 to 5804) AUTHORS Buchel,D.E., Gronenborn,B. and Muller-Hill,B. TITLE Sequence of the lactose permease gene JOURNAL Nature 283 (5747), 541-545 (1980) PUBMED 6444453 REFERENCE 16 (sites) AUTHORS Miller,J.H., Calos,M.P. and Galas,D.J. TITLE Genetic and sequencing studies of the specificity of transposition into the lac region of E. coli JOURNAL Cold Spring Harb. Symp. Quant. Biol. 45 (PT 1), 243-257 (1981) PUBMED 6271472 REFERENCE 17 (sites) AUTHORS Chenchick,A., Beabealashvilli,R. and Mirzabekov,A. TITLE Topography of interaction of Escherichia coli RNA polymerase subunits with lac UV5 promoter JOURNAL FEBS Lett. 128 (1), 46-50 (1981) PUBMED 7023981 REFERENCE 18 (sites) AUTHORS Betz,J.L. and Sadler,J.R. TITLE Variants of a cloned synthetic lactose operator. I. A palindromic dimer lactose operator derived from one stand of the cloned 40-base pair operator JOURNAL Gene 13 (1), 1-12 (1981) PUBMED 7016667 REFERENCE 19 (sites) AUTHORS Sadler,J.R. and Tecklenburg,M. TITLE Cloning and characterization of the natural lactose operator JOURNAL Gene 13 (1), 13-23 (1981) PUBMED 6263752 REFERENCE 20 (sites) AUTHORS Betz,J.L. and Sadler,J.R. TITLE Variants of a cloned synthetic lactose operator. II. Chloramphenicol-resistant revertants retaining a lactose operator in the CAT gene of plasmid pBR325 JOURNAL Gene 15 (2-3), 187-200 (1981) PUBMED 6271642 REFERENCE 21 (sites) AUTHORS Calos,M.P. and Miller,J.H. TITLE The DNA sequence change resulting from the IQ1 mutation, which greatly increases promoter strength JOURNAL Mol. Gen. Genet. 183 (3), 559-560 (1981) PUBMED 7038381 REFERENCE 22 (sites) AUTHORS Mieschendahl,M., Buchel,D., Bocklage,H. and Muller-Hill,B. TITLE Mutations in the lacY gene of Escherichia coli define functional organization of lactose permease JOURNAL Proc. Natl. Acad. Sci. U.S.A. 78 (12), 7652-7656 (1981) PUBMED 6278484 REFERENCE 23 (sites) AUTHORS Russell,D.R. and Bennett,G.N. TITLE Construction and analysis of in vivo activity of E. coli promoter hybrids and promoter mutants that alter the -35 to -10 spacing JOURNAL Gene 20 (2), 231-243 (1982) PUBMED 6299890 REFERENCE 24 (sites) AUTHORS Horowitz,H. and Platt,T. TITLE A termination site for LacI transcription is between the CAP site and the lac promoter JOURNAL J. Biol. Chem. 257 (19), 11740-11746 (1982) PUBMED 6288696 REFERENCE 25 (sites) AUTHORS Klein,R.D. and Wells,R.D. TITLE Effects of neighboring DNA homopolymers on the biochemical and physical properties of the Escherichia coli lactose promoter. I. Cloning and characterization studies JOURNAL J. Biol. Chem. 257 (21), 12954-12961 (1982) PUBMED 6290487 REFERENCE 26 (bases 1183 to 1291) AUTHORS Weiher,H. and Schaller,H. TITLE Segment-specific mutagenesis: extensive mutagenesis of a lac promoter/operator element JOURNAL Proc. Natl. Acad. Sci. U.S.A. 79 (5), 1408-1412 (1982) PUBMED 7041119 REFERENCE 27 (sites) AUTHORS Van Dyke,M.W. and Dervan,P.B. TITLE Footprinting with MPE.Fe(II). Complementary-strand analyses of distamycin- and actinomycin-binding sites on heterogeneous DNA JOURNAL Cold Spring Harb. Symp. Quant. Biol. 47 (PT 1), 347-353 (1983) PUBMED 6305557 REFERENCE 28 (bases 1287 to 4364) AUTHORS Kalnins,A., Otto,K., Ruther,U. and Muller-Hill,B. TITLE Sequence of the lacZ gene of Escherichia coli JOURNAL EMBO J. 2 (4), 593-597 (1983) PUBMED 6313347 REFERENCE 29 (sites) AUTHORS Cone,K.C., Sellitti,M.A. and Steege,D.A. TITLE Lac repressor mRNA transcription terminates in vivo in the lac control region JOURNAL J. Biol. Chem. 258 (18), 11296-11304 (1983) PUBMED 6309841 REFERENCE 30 (sites) AUTHORS Sadler,J.R., Sasmor,H. and Betz,J.L. TITLE A perfectly symmetric lac operator binds the lac repressor very tightly JOURNAL Proc. Natl. Acad. Sci. U.S.A. 80 (22), 6785-6789 (1983) PUBMED 6316325 REFERENCE 31 (sites) AUTHORS Glickman,B.W. and Ripley,L.S. TITLE Structural intermediates of deletion mutagenesis: a role for palindromic DNA JOURNAL Proc. Natl. Acad. Sci. U.S.A. 81 (2), 512-516 (1984) PUBMED 6582506 REFERENCE 32 (sites) AUTHORS Spassky,A., Kirkegaard,K. and Buc,H. TITLE Changes in the DNA structure of the lac UV5 promoter during formation of an open complex with Escherichia coli RNA polymerase JOURNAL Biochemistry 24 (11), 2723-2731 (1985) PUBMED 3896305 REFERENCE 33 (sites) AUTHORS Straney,D.C. and Crothers,D.M. TITLE Intermediates in transcription initiation from the E. coli lac UV5 promoter JOURNAL Cell 43 (2 PT 1), 449-459 (1985) PUBMED 2416465 REFERENCE 34 (sites) AUTHORS Looman,A.C., de Gruyter,M., Vogelaar,A. and van Knippenberg,P.H. TITLE Effects of heterologous ribosomal binding sites on the transcription and translation of the lacZ gene of Escherichia coli JOURNAL Gene 37 (1-3), 145-154 (1985) PUBMED 3932130 REFERENCE 35 (sites) AUTHORS Mandecki,W., Goldman,R.A., Powell,B.S. and Caruthers,M.H. TITLE lac Up-promoter mutants with increased homology to the consensus promoter sequence JOURNAL J. Bacteriol. 164 (3), 1353-1355 (1985) PUBMED 2999082 REFERENCE 36 (sites) AUTHORS Malamy,M.H., Rahaim,P.T., Hoffman,C.S., Baghdoyan,D., O'Connor,M.B. and Miller,J.F. TITLE A frameshift mutation at the junction of an IS1 insertion within lacZ restores beta-galactosidase activity via formation of an active lacZ-IS1 fusion protein JOURNAL J. Mol. Biol. 181 (4), 551-555 (1985) PUBMED 2987506 REFERENCE 37 (bases 5646 to 7477) AUTHORS Hediger,M.A., Johnson,D.F., Nierlich,D.P. and Zabin,I. TITLE DNA sequence of the lactose operon: the lacA gene and the transcriptional termination region JOURNAL Proc. Natl. Acad. Sci. U.S.A. 82 (19), 6414-6418 (1985) PUBMED 3901000 COMMENT Original source text: Escherichia coli DNA; mRNA; clone lambda-h80dlac DNA; clone puk217; pgm8 (see comment). [3] sites; UV5 mRNA transcripts and operator mutants. [(in) Sund,H. and Blauer,G. (eds.);Protein-Ligand Interactions: 193-207;Walter de] sites; operator mutational analysis. [7] sites; S1 and mung bean nuclease action on operator DNA. [9] sites; class I, II and III promoter mutant analysis. [13] sites; lacI mutant analysis. [16] sites; Tn5, Tn9 and Tn10 insertion sites in lac region. [14] sites; lacI promoter mutation UJ177. [18] sites; palindromic dimer operator;. [19] sites; natural operator sequence. [20] sites; operator mutational analysis. [21] sites; lacI-Q deletion. [17] sites; RNA polymerase UV5 promoter interaction. [22] sites; lacY mutational analysis. [24] sites; lacI-promoted transcription termination. [25] sites; wt and UV5 promoter sequence studies. [23] sites; UV5 promoter mutational analysis. [30] sites; perfectly symmetric operator sequence. [29] sites; lacI mRNA termination site. [27] sites; distamycin and actinomycin binding to promoter. [31] sites; lacI deletion studies. [35] sites; promoter mutational studies. [33] sites; DNAase I studies with promoter sequence. [34] sites; ribosomal binding and translation initiation for lacZ. [36] sites; insertion sequence IS1 integration in lacZ;. [32] sites; DNAase I studies with promoter. [1] first reports a 27 bp operator(sites 1240-1266) with two-fold symmetries; the operator has also been defined to be bases 1246-1266 or bases 1239-1273 [8]. [(in) Kjeldgaard,N.C. and Maaloe, O.(eds);Control of ribosome synthesis: 138-143;A] explores the ability of lac repressor protein to affect methylation of operator DNA. [8] argues that DNA on both sides of the 21 bp operator (bases 1246-1266) affects repressor binding but that the sequences of this DNA are probably not critical. [5] gives a larger sequence known as the promoter-operator region for the wild-type, whereas [2] and [26] give portions of this region for the mutant strain UV5. Within the promoter region, bases 1162-1199 are identified as the catabolite gene activator protein binding site (cap) and bases 1200-1245 are the RNA polymerase interaction site. [10] reports a sequence for the 5'end of the lacI (repressor) gene and discusses restart in mutant strains. [11] presents a sequence for the lacI promoter region and identifies an I-Q mutation which enhances lacI transcription approximately ten-fold. [12] gives a complete sequence for lacI which agrees with the known lac repressor sequence. [26] examines the promoter-operator region in the UV5 strain (lac109) and studies 23 mutant derivatives of this sequence. This sequence agrees with known protein sequences for the lacZ, lacY and lacA enzymes. [15] notes that the fMet codon is not present for lacA and suggests that the 'ttg' codon (5727-5729), which immediately precedes the mature N-terminal asparagine codon, is the start codon. The cds for lacZ, lacY and lacA are included on a single mRNA transcript. Complete source information: Escherichia coli DNA [1],[(in) Kjeldgaard,N.C. and Maaloe,O.(eds); Control of ribosome synthesis: 138-143;A],[8],[12],[26]; mRNA [2], [5],[10]; clone lambda-h80dlac DNA [11],[15]; clone puk217 [28]; pgm8 [37]. FEATURES Qualifiers source /organism="Escherichia coli" /mol_type="genomic DNA" /db_xref="taxon:562" protein /gene="lacI" /note="lac repressor protein (gtg start codon)" /transl_table=11 BEGIN 1 MKPVTLYDVA EYAGVSYQTV SRVVNQASHV SAKTREKVEA AMAELNYIPN RVAQQLAGKQ 61 SLLIGVATSS LALHAPSQIV AAIKSRADQL GASVVVSMVE RSGVEACKAA VHNLLAQRVS 121 GLIINYPLDD QDAIAVEAAC TNVPALFLDV SDQTPINSII FSHEDGTRLG VEHLVALGHQ 181 QIALLAGPLS SVSARLRLAG WHKYLTRNQI QPIAEREGDW SAMSGFQQTM QMLNEGIVPT 241 AMLVANDQMA LGAMRAITES GLRVGADISV VGYDDTEDSS CYIPPSTTIK QDFRLLGQTS 301 VDRLLQLSQG QAVKGNQLLP VSLVKRKTTL APNTQTASPR ALADSLMQLA RQVSRLESGQ //