LOCUS       SIU02516.1               489 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 MONOOXYGENASE
            ETHA protein.
ACCESSION   LT708304-3930
PROTEIN_ID  SIU02516.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="ethA"
                     /locus_tag="BQ2027_MB3884C"
                     /note="Mb3884c, ethA, len: 489 aa. Equivalent to Rv3854c,
                     len: 489 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 489 aa overlap). ethA (alternate gene
                     names: aka or etaA), monooxygenase required for activation
                     of the pro-drug ethionamide (EC 1.-.-.-) (see citations
                     below), highly similar to other monooxygenases e.g.
                     Q9A588|CC2569 MONOOXYGENASE (FLAVIN-BINDING FAMILY) from
                     Caulobacter crescentus (498 aa), FASTA scores: opt: 1959,
                     E(): 2.9e-114, (57.6% identity in 481 aa overlap);
                     Q9RZT0|DRB0033 ARYLESTERASE/MONOXYGENASE from Deinococcus
                     radiodurans (833 aa), FASTA scores: opt: 1771, E():
                     2.2e-102, (53.75% identity in 480 aa overlap);
                     Q9A8K5|CC1348 MONOOXYGENASE (FLAVIN-BINDING FAMILY) from
                     Caulobacter crescentus (499 aa), FASTA scores: opt: 1385,
                     E(): 1.4e-78, (43.2% identity in 486 aa overlap); etc.
                     Also highly similar to others from Mycobacterium
                     tuberculosis e.g. O53300|Rv3083|MTV013.04 MONOXYGENASE
                     (495 aa) FASTA scores: opt: 1692, E(): 1.1e-97, (49.7%
                     identity in 489 aa overlap); O53762|Rv0565c|MTV039.03c
                     PUTATIVE MONOXYGENASE (486 aa), FASTA scores: opt: 1571,
                     E(): 3.7e-90, (49.05% identity in 471 aa overlap);
                     O69708|Rv3741c|MTV025.089c POSSIBLE OXIDOREDUCTASE
                     (probably second part of a two component monooxygenase)
                     (224 aa), FASTA scores: opt: 542, E(): 1.7e-26, (50.0%
                     identity in 162 aa overlap); etc. Protein product from
                     Mb3884c detected using shotgun mass spectrometry and SWATH
                     mass spectrometry. Mb3884c found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:Q7TVI2"
                     /db_xref="InterPro:IPR020946"
                     /db_xref="InterPro:IPR036188"
                     /db_xref="UniProtKB/Swiss-Prot:Q7TVI2"
BEGIN
        1 MTEHLDVVIV GAGISGVSAA WHLQDRCPTK SYAILEKRES MGGTWDLFRY PGIRSDSDMY
       61 TLGFRFRPWT GRQAIADGKP ILEYVKSTAA MYGIDRHIRF HHKVISADWS TAENRWTVHI
      121 QSHGTLSALT CEFLFLCSGY YNYDEGYSPR FAGSEDFVGP IIHPQHWPED LDYDAKNIVV
      181 IGSGATAVTL VPALADSGAK HVTMLQRSPT YIVSQPDRDG IAEKLNRWLP ETMAYTAVRW
      241 KNVLRQAAVY SACQKWPRRM RKMFLSLIQR QLPEGYDVRK HFGPHYNPWD QRLCLVPNGD
      301 LFRAIRHGKV EVVTDTIERF TATGIRLNSG RELPADIIIT ATGLNLQLFG GATATIDGQQ
      361 VDITTTMAYK GMMLSGIPNM AYTVGYTNAS WTLKADLVSE FVCRLLNYMD DNGFDTVVVE
      421 RPGSDVEERP FMEFTPGYVL RSLDELPKQG SRTPWRLNQN YLRDIRLIRR GKIDDEGLRF
      481 AKRPAPVGV
//