LOCUS       SIU02076.1               212 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            ALTERNATIVE RNA POLYMERASE SIGMA-D FACTOR SIGD protein.
ACCESSION   LT708304-3490
PROTEIN_ID  SIU02076.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="sigD"
                     /locus_tag="BQ2027_MB3448C"
                     /note="Mb3448c, sigD, len: 212 aa. Equivalent to Rv3414c,
                     len: 212 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 212 aa overlap). Probable sigD,
                     alternative RNA polymerase sigma-D factor (see citation
                     below), similar to others (notably from Streptomyces
                     coelicolor) e.g. Q9L0I8|SCD63.01 from Streptomyces
                     coelicolor (195 aa), FASTA scores: opt: 533, E(): 9.6e-28,
                     (47.25% identity in 182 aa overlap); Q9FDS3|ADSA from
                     Streptomyces griseus (258 aa), FASTA scores: opt: 223,
                     E(): 1.8e-07, (28.95% identity in 183 aa overlap);
                     BAB48649|MLL1224 from Rhizobium loti (Mesorhizobium loti)
                     (187 aa), FASTA scores: opt: 202, E(): 3.2e-06, (30.4%
                     identity in 194 aa overlap);
                     P38133|RPOE_STRCO|SIGE|SCE94.07 from Streptomyces
                     coelicolor (176 aa), FASTA scores: opt: 200, E(): 4.1e-06,
                     (35.25% identity in 156 aa overlap); P37978|CNRH_ALCEU
                     from Alcaligenes eutrophus (Ralstonia eutropha), FASTA
                     scores: opt: 197, E(): 6.9e-06, (30.35% identity in 191 aa
                     overlap); etc. C-terminus strongly similar to N-terminal
                     part of Q49727|S1620B|B1620_C3_233 HYPOTHETICAL 6.2 KDA
                     PROTEIN from Mycobacterium leprae (59 aa), FASTA scores:
                     opt: 217, E(): 1.3e-07, (90.25% identity in 41 aa
                     overlap). BELONGS TO THE SIGMA-70 FACTOR FAMILY. Protein
                     product from Mb3448c detected using SWATH mass
                     spectrometry. Mb3448c found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /db_xref="GOA:P66812"
                     /db_xref="InterPro:IPR000838"
                     /db_xref="InterPro:IPR007627"
                     /db_xref="InterPro:IPR013249"
                     /db_xref="InterPro:IPR013324"
                     /db_xref="InterPro:IPR013325"
                     /db_xref="InterPro:IPR014284"
                     /db_xref="InterPro:IPR036388"
                     /db_xref="InterPro:IPR039425"
                     /db_xref="UniProtKB/Swiss-Prot:P66812"
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MVDPGVSPGC VRFVTLEISP SMTMQGERLD AVVAEAVAGD RNALREVLET IRPIVVRYCR
       61 ARVGTVERSG LSADDVAQEV CLATITALPR YRDRGRPFLA FLYGIAAHKV ADAHRAAGRD
      121 RAYPAETLPE RWSADAGPEQ MAIEADSVTR MNELLEILPA KQREILILRV VVGLSAEETA
      181 AAVGSTTGAV RVAQHRALQR LKDEIVAAGD YA
//