LOCUS       SIU02030.1               154 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            tRNA/rRNA METHYLASE SPOU (tRNA/rRNA METHYLTRANSFERASE)
            protein.
ACCESSION   LT708304-3444
PROTEIN_ID  SIU02030.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="spoU"
                     /locus_tag="BQ2027_MB3401"
                     /note="Mb3401, spoU, len: 154 aa. Equivalent to Rv3366,
                     len: 154 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 154 aa overlap). Probable spoU,
                     tRNA/rRNA methylase (EC 2.1.1.-), equivalent to
                     Q9CCU7|ML0419 PUTATIVE tRNA/rRNA METHYLTRANSFERASE from
                     Mycobacterium leprae (158 aa), FASTA scores: opt: 861,
                     E(): 1.2e-50, (83.75% identity in 154 aa overlap); and
                     O07698|MLCL383.24c rRNA METHYLASE from Mycobacterium
                     leprae (169 aa), FASTA scores: opt: 861, E(): 1.3e-50,
                     (83.75% identity in 154 aa overlap). Also highly similar
                     to many members of the spoU family of rRNA methylases e.g.
                     Q9K199|NMB0268 RNA METHYLTRANSFERASE (TRMH FAMILY) from
                     Neisseria meningitidis (serogroup B) (154 aa), FASTA
                     scores: opt: 534, E(): 7.6e-29, (50.0% identity in 154 aa
                     overlap); and Q9JSM8|NMA2218 from Neisseria meningitidis
                     (serogroup A) (154 aa), FASTA scores: opt: 526, E():
                     2.6e-28, (49.35% identity in 154 aa overlap);
                     Q9HU57|PA5127 from Pseudomonas aeruginosa (153 aa), FASTA
                     scores: opt: 531, E(): 1.2e-28, (52.95% identity in 151 aa
                     overlap); P33899|YIBK_ECOLI|B3606 from Escherichia coli
                     strain K12 (157 aa), FASTA scores: opt: 511, E(): 2.6e-27,
                     (49.35% identity in 154 aa overlap); etc. BELONGS TO THE
                     RNA METHYLTRANSFERASE TRMH FAMILY. Protein product from
                     Mb3401 detected using SWATH mass spectrometry. Mb3401
                     found to be expressed during exponential growth in
                     Sauton's minimal media by RNA-sequencing (TPM > 20)."
                     /db_xref="GOA:A0A1R3Y407"
                     /db_xref="InterPro:IPR001537"
                     /db_xref="InterPro:IPR016914"
                     /db_xref="InterPro:IPR029026"
                     /db_xref="InterPro:IPR029028"
                     /db_xref="UniProtKB/TrEMBL:A0A1R3Y407"
BEGIN
        1 MFRLLFVSPR IAPNTGNAIR TCAATGCELH LVEPLGFDLS EPKLRRAGLD YHDLASVTVH
       61 ASLAHAWEAL SPARVFAFTA QATTLFTNVG YRAGDVLMFG PEPTGLDEAT LADTHITGQV
      121 RIPMLAGRRS LNLSNAAAVA VYEAWRQHGF AGAV
//